Which isoform of Neurofascin is 8208-NF?

Name: Recombinant Human Neurofascin Protein, CF
Brand: R&D Systems
SKU: 8208-NF
Price: 394 USD

8208-NF corresponds to Accession number O94856-3. Accession number O94856-3 is highly similar to O94856-8, which is also known as NF155. Isoform NF140 contains FNIII domains 1, 2, and 4, and no mucin domain, but it lacks FNIII domain 3. Because 8208-NF contains FNIII domain 3, 8208-NF is not isoform NF140. 8208-NF is actually closest to NF155

Recombinant Human Neurofascin Protein, CF

Catalog # 8208-NF | R&D Systems, Inc. a Bio-Techne Brand

Citations (2)

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Key Product Details

Source:	HEK293
Accession #:	NP_055905.2
Applications:	Bioactivity

View Full Technical Details for Recombinant Human Neurofascin Protein, CF

Product Specifications for Recombinant Human Neurofascin Protein, CF

Source	Human embryonic kidney cell, HEK293-derived human Neurofascin protein Ile25-Trp1039, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE with silver staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ile25
Predicted Molecular Mass	116 kDa
SDS-PAGE	120-140 kDa, reducing conditions
Activity	Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Recombinant Human Neurofascin, immobilized at 6 μg/mL on a 96-well plate, is able to significantly enhance neurite outgrowth.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8208-NF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: Neurofascin

Neurofascin, a type I transmembrane glycoprotein, is a member of the L1 family of cell adhesion molecules (CAMs) (1-4). L1CAM family members are composed of an extracellular domain (ECD) that contains six immunoglobulin (Ig)-like domains and multiple fibronectin type III repeats, followed by transmembrane and cytoplasmic domains (2, 4, 5). Multiple isoforms of Neurofascin, including NF155, NF166, NF180, and NF186, can be generated by alternative splicing with a predicted range of approximately 70 kDa to 150 kDa (4). These isoforms differ in the combination of fibronectin type III repeats, as well as in the presence of a proline-, alanine-, and threonine-rich segment (PAT domain) located just after the fourth fibronectin type III repeat (4). This recombinant human Neurofascin protein is most close to NF155 and shares 96% amino acid sequence identity with comparable regions of rat and mouse homologs. In rats, NF155 is transiently expressed by oligodendrocytes and Schwann cells during axon myelination (6, 7). NF155 clusters in paranodal regions of oligodendroglia and binds to the Caspr-Contactin complex located on the adjacent axon to form and stabilize paranodal axoglial junctions (8-11). It has been suggested that the ECD of NF155 must be cleaved from oligodendroglia membranes to form and/or stabilize the paranodal structure (12). NF155 has also been shown to promote neuronal adhesion and neurite outgrowth in rats and chickens (12-14). Alterations in NF155 expression have been associated with multiple sclerosis (15).

Item	Value
References	Rathjen, F.G. et al. (1987) Cell 51:841. Volkmer, H. et al. (1992) J. Cell Biol. 118:149. Sherman, D.L. and P.J. Brophy (2005) Nat. Rev. Neurosci. 6:683. Kriebel, M. et al. (2012) Int. J. Biochem. Cell Biol. 44:694. Liu, H. et al. (2011) J. Biol. Chem. 286:797. Collinson, J.M. et al. (1998) Glia 23:11. Basak, S. et al. (2007) Dev. Biol. 311:408. Charles, P. et al. (2002) Curr. Biol. 12:217. Schafer, D.P. et al. (2004) J. Neurosci. 24:3176. Maier, O. et al. (2005) Mol. Cell. Neurosci. 28:390. Sherman, D.L. et al. (2005) Neuron 48:737. Maier, O. et al. (2006) Exp. Cell Res. 312:500. Volkmer, H. et al. (1996) J. Cell Biol. 135:1059. Koticha, D. et al. (2005) Mol. Cell. Neurosci. 30:137. Howell, O.W. et al. (2006) Brain 129:3173.
Entrez Gene IDs	23114 (Human); 269116 (Mouse); 116690 (Rat)
Alternate Names	DKFZp686P2250, KIAA0756FLJ46866, NF, NFASC, NRCAML, Neurofascin, neurofascin homolog, neurofascin homolog (chicken)

Citations for Recombinant Human Neurofascin Protein, CF (2)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Neurofascin Protein, CF include:

Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA(Detection)

AJ Davies et al. (2020-06-27), Immunoadsorption and Plasma Exchange in Seropositive and Seronegative Immune-Mediated Neuropathies J Clin Med, 2020-09(7).
PMID: 32605107
Species: Human
Sample Types: Serum
Applications: Bioassay

Katsunori Ikewaki (2016-11-09), IgG4 anti-neurofascin155 antibodies in chronic inflammatory demyelinating polyradiculoneuropathy: Clinical significance and diagnostic utility of a conventional assay J. Neuroimmunol., 2016-10(0).
PMID: 27852440

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FAQs for Recombinant Human Neurofascin Protein, CF

Which isoform of Neurofascin is 8208-NF?

8208-NF corresponds to Accession number O94856-3. Accession number O94856-3 is highly similar to O94856-8, which is also known as NF155. Isoform NF140 contains FNIII domains 1, 2, and 4, and no mucin domain, but it lacks FNIII domain 3. Because 8208-NF contains FNIII domain 3, 8208-NF is not isoform NF140. 8208-NF is actually closest to NF155

Recombinant Human Neurofascin Protein, CF

Catalog # 8208-NF | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Product Specifications for Recombinant Human Neurofascin Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: Neurofascin

Citations for Recombinant Human Neurofascin Protein, CF (2)

FAQs for Recombinant Human Neurofascin Protein, CF

Product Documents for Recombinant Human Neurofascin Protein, CF

Product Datasheets

COA

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SDS

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