Chinese Hamster Ovary cell line, CHO-derived human Netrin-G2 protein Asp18-Pro506, with a C-terminal 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
70-78 kDa, under reducing conditions
Measured by its binding ability in a functional ELISA. When Recombinant Human Netrin‑G2 His-Tag protein is coated at 1 µg/mL, Recombinant Human LRRC4 Fc Chimera (Catalog # 9375-LR) binds with an ED50 of 1.5-9 ng/mL.
Recombinant Human Netrin-G2 His-tag Protein, CF Scientific Data Examples
Recombinant Human Netrin-G2 His-tag Protein Binding Activity.
When Recombinant Human Netrin‑G2 His-Tag (Catalog # 10675-NG) is coated at 1 µg/mL, Recombinant Human LRRC4 Fc Chimera (9375-LR) binds with an ED50 of 1.5-9 ng/mL.
Recombinant Human Netrin-G2 His-tag Protein SDS-PAGE.
2 μg/lane of Recombinant Human Netrin-G2 His-tag (Catalog # 10675-NG) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 70-78 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 1 mg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
NTNG-2 (Netrin-G2) is a member of a GPI-linked Netrin subfamily that is distantly related to classical Netrins. Mature human Netrin-G2 is approximately 490 amino acid (aa) in length. It contains an N-terminal laminin-related region, several epidermal-growth-factor-like domains and a heparin-binding C-terminus that ends in a GPI-linkage (1). The laminin-related region shows a globular type VI domain followed by three variable-length EGF-like domains. Mature human Netrin-G2 shares 96% aa identity with mouse and rat Netrin-G2. NTNG-2 is predominantly expressed in specific neuronal subsets of the developing and mature central nervous system (2). The protein interacts with the extracellular region of their specific Netrin-G ligand receptors LRRC4 (3). The netrin family proteins provide axonal guidance cues during central nervous system development (4).
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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