Source	Human embryonic kidney cell, HEK293-derived human Netrin-G1a protein His29-Gly503, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Val19 & His29
Predicted Molecular Mass	55 kDa
SDS-PAGE	69-76 kDa, reducing conditions
Activity	Measured by its binding ability in a functional ELISA. When Recombinant Human NGL-1/LRRC4C (Catalog # 4899-NR) is coated at 2 μg/mL (100 μL/well), the concentration of Recombinant Human Netrin-G1a that produces 50% optimal binding response is 0.2-1 μg/mL.

Recombinant Human Netrin-G1a His-tag Protein, CF Scientific Data Examples

	Recombinant Human Netrin-G1a His-tag Protein Binding Activity When Recombinant Human NGL-1/LRRC4C (Catalog # 4899-NR) is coated at 2 µg/mL, 100 µL/well, Recombinant Human Netrin-G1a (Catalog # 10019-NG) binds with an ED₅₀ of 0.2-1 µg/mL.
	Recombinant Human Netrin-G1a His-tag Protein SDS-PAGE 2 μg/lane of Recombinant Human Netrin‑G1a was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 69-76 kDa and 55- 66 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10019-NG

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Netrin-G1a

NTNG-1, known as Netrin-G1 or alternatively Laminet-1 (Gene name: NTNG1), is a member of the UNC-6/Netrin family of proteins. Human Netrin-G1 is a 55 kDa protein and shares 100% homology with its crab-eating macaque, 98% homology with its rat homolog, and 97% homology with its mouse homolog. Netrin-G1 is synthesized as a 539 amino acid (aa) precursor with a 28 aa signal sequence, a 251 aa laminin-related region containing an N-terminal laminin globular domain (domain VI) followed by 3 laminin EGF-like repeats, a GPI (glycosylphosphatidylinisotol) anchor (Ser510), and a C-terminal 28 aa propeptide that is removed in mature Netrin-G1 (1, 3, 4). Netrin-G1 interacts with its ligand, Netrin-G ligand 1 (NGL1) to regulate neuron growth and patterning, axonal subdendritic differentiation, and synapse formation throughout development (1, 2, 4, 6). Abnormal expression of Netrin-G1 via SNPs (single nucleotide polymorphisms) have been implicated in pathogenesis for schizophrenia (5). Netrin-G1 has widespread expression only in vertebrates, occurring in olfactory mitral cells, cells of the inferior colliculus (hearing), dorsal thalamus (behavior), and cells of the deep cerebellar nuclei and inferior olive (motion) (3).

Item	Value
References	Seiradake, E. et al. (2011) EMBO J. 30:4479. Song, Y. et al. (2013) J Cell Sci. 126:4926. Nakashiba, T. et al. (2000) J Neuroscience. 20:6540. Matsukawa, H. et al. (2014) J Neuroscience. 34:15779. Zhu, Y. et al. (2011) J Genet. 90:499. Nishimura-Akiyoshi, S. et al. (2007) Neuroscience. 104:14801.
Entrez Gene IDs	22854 (Human); 80883 (Mouse)
Alternate Names	Lmnt1, NTG1, NTNG1, Netrin-G1a, NetrinG1a

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Netrin-G1a His-tag Protein, CF include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

N Naldaiz-Ga et al. (2019), Isolation and characterization of myogenic precursor cells from human cremaster muscle Sci Rep, 9(1):3454.
PMID: 30837559

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Recombinant Human Netrin-G1a His-tag Protein, CF

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