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Recombinant Human Netrin-G1a His-tag Protein, CF

Catalog # 10019-NG | R&D Systems, Inc. a Bio-Techne Brand
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10019-NG-050

Key Product Details

Source

HEK293

Accession #

Structure / Form

Monomer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Netrin-G1a protein
His29-Gly503, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val19 & His29

Predicted Molecular Mass

55 kDa

SDS-PAGE

69-76 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human NGL-1/LRRC4C (Catalog # 4899-NR) is coated at 2 μg/mL (100 μL/well), the concentration of Recombinant Human Netrin-G1a that produces 50% optimal binding response is 0.2-1 μg/mL.

Scientific Data Images for Recombinant Human Netrin-G1a His-tag Protein, CF

Recombinant Human Netrin-G1a His-tag Protein Binding Activity

Recombinant Human Netrin-G1a His-tag Protein Binding Activity

When Recombinant Human NGL-1/LRRC4C (Catalog # 4899-NR) is coated at 2 µg/mL, 100 µL/well, Recombinant Human Netrin-G1a (Catalog # 10019-NG) binds with an ED50 of 0.2-1 µg/mL.
Recombinant Human Netrin-G1a His-tag Protein SDS-PAGE

Recombinant Human Netrin-G1a His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human Netrin‑G1a was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 69-76 kDa and 55- 66 kDa, respectively.

Formulation, Preparation and Storage

10019-NG
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: Netrin-G1a

NTNG-1, known as Netrin-G1 or alternatively Laminet-1 (Gene name: NTNG1), is a member of the UNC-6/Netrin family of proteins. Human Netrin-G1 is a 55 kDa protein and shares 100% homology with its crab-eating macaque, 98% homology with its rat homolog, and 97% homology with its mouse homolog. Netrin-G1 is synthesized as a 539 amino acid (aa) precursor with a 28 aa signal sequence, a 251 aa laminin-related region containing an N-terminal laminin globular domain (domain VI) followed by 3 laminin EGF-like repeats, a GPI (glycosylphosphatidylinisotol) anchor (Ser510), and a C-terminal 28 aa propeptide that is removed in mature Netrin-G1 (1, 3, 4). Netrin-G1 interacts with its ligand, Netrin-G ligand 1 (NGL1) to regulate neuron growth and patterning, axonal subdendritic differentiation, and synapse formation throughout development (1, 2, 4, 6). Abnormal expression of Netrin-G1 via SNPs (single nucleotide polymorphisms) have been implicated in pathogenesis for schizophrenia (5). Netrin-G1 has widespread expression only in vertebrates, occurring in olfactory mitral cells, cells of the inferior colliculus (hearing), dorsal thalamus (behavior), and cells of the deep cerebellar nuclei and inferior olive (motion) (3).

References

  1. Seiradake, E. et al. (2011) EMBO J. 30:4479.
  2. Song, Y. et al. (2013) J Cell Sci. 126:4926.
  3. Nakashiba, T. et al. (2000) J Neuroscience. 20:6540.
  4. Matsukawa, H. et al. (2014) J Neuroscience. 34:15779.
  5. Zhu, Y. et al. (2011) J Genet. 90:499.
  6. Nishimura-Akiyoshi, S. et al. (2007) Neuroscience. 104:14801.

Alternate Names

NetrinG1a, NTG1, NTNG1

Entrez Gene IDs

22854 (Human); 80883 (Mouse)

Gene Symbol

NTNG1

UniProt

Additional Netrin-G1a Products

Product Documents for Recombinant Human Netrin-G1a His-tag Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Netrin-G1a His-tag Protein, CF

For research use only

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