Product Specifications for Recombinant Human NETO2 His-tag Protein, CF
Mouse myeloma cell line, NS0-derived human NETO2 protein Ile23-Lys345, with a C-terminal 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
32-45 kDa, reducing conditions.
Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Recombinant Human NETO2 His-tag, immobilized at 2.5 μg/mL on a 96 well plate, is able to significantly enhance neurite outgrowth.
Scientific Data Examples for Recombinant Human NETO2 His-tag Protein, CF
Recombinant Human NETO2 His-tag Protein SDS-PAGE.
2 μg/lane of Recombinant Human NETO2 His-tag Protein (Catalog # 10975-NO) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 32-45 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 500 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
NETO2, or Neuropilin and tolloid-like protein 2, is a type 1 transmembrane protein belonging to the CUB (for complement C1r/C1s, Ueqf, Bmp1) domain and LDLa‑containing protein family that modulates the neuronal kainate receptors (KARs) (1-3). It is mainly expressed in neural tissues but was also reported to be expressed in several non-neural tissues and its expression has been associated with various cancers such as renal, lung, colon, cervical and colorectal cancers (4-6). Mature human NETO2 is composed of a 325 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 157 aa cytoplasmic region. Within the ECD, human NETO2 shares 98% aa identity with mouse and rat NETO2. It functions as an accessory subunit of neuronal kainate-sensitive glutamate receptors, GRIK2 and GRIK3 regulating channel activities and synaptic transmission (7).
Fedorova, M.S. et al. (2020) Front. Genet. 11:594933. doi: 10.3389/fgene.2020.594933.
Stohr, H. et al. (2002) Gene 286:223.
Straub, C. et al. (2011) J. Neurosci. 31:8078.
Li, Y. et al. (2019) Cancer Manag. Res. 11:5147.
Niu, O. et al. (2012) Genetika. 48:506.
Xu, J. et al. (2021) Int. J. Biol. Sci. 17:259.
Griffith T. et al. (2015) J. Physiol. 593:4815.
Neuropilin [NRP] And Tolloid [TLL]-like 2
Entrez Gene IDs
81831 (Human); 74513 (Mouse); 307757 (Rat)
BTCL2, Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor classA domains protein 2, FLJ10430, FLJ90456, NEOT2, NEOT2FLJ14724, NETO2, neuropilin (NRP) and tolloid (TLL)-like 2, neuropilin and tolloid-like protein 2
Citations for Recombinant Human NETO2 His-tag Protein, CF
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Product Documents for Recombinant Human NETO2 His-tag Protein, CF
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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