Chinese Hamster Ovary cell line, CHO-derived human NETO1 protein Thr23-Val345, with a C-terminal 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
34-48 kDa, under reducing conditions.
Measured by its binding ability in a functional ELISA. When Recombinant Human NETO1 His-tag Protein is immobilized at 4.00 µg/mL (100 µL/well), Recombinant Human APP695+1 Fc Chimera binds with an ED50 of 0.200-1.20 μg/mL.
Recombinant Human NETO1 His-tag Protein, CF Scientific Data Examples
Recombinant Human NETO1 His-tag Protein SDS-PAGE
2 μg/lane of Recombinant Human NETO1 His-tag Protein (Catalog # 11103-NO) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 34-48 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitute at 500 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Neuropilin and tolloid-like protein 1 (NETO1), also known as Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 1 (BTCL1), is a type I transmembrane protein that is expressed in the brain and retina (1). The 511 amino acid (aa) residue mature human NETO1 contains a 322 aa extracellular domain (ECD) with two CUB domains and one class A LDL-receptor segment, a 21 aa transmembrane domain, and a 168 aa cytosol domain (1). Three human NETO1 splice isoforms exist, including a secreted soluble variant that shows an Asp-to-Glu transition at residue 157 followed by a premature truncation (1). The ECD of human NETO1 shares 97% and 98% aa identity with that of mouse and canine NETO1, respectively. NETO1 is expressed in the human brain and retina (1, 2). It increases the rate of GluK1 receptor desensitization and is a candidate NMDAR auxiliary subunit regulating channel activities and synaptic transmission (3, 4). NETO1 expression is associated with metastasis and poor survival in epithelial ovarian cancer (5).
Stöhr, H. et al. (2002) Gene 286:223.
Tomita, S. and P.E. Castillo (2012) J. Physiol. 590:2217.
Copits, B.A. et al. (2011) J. Neurosci. 31:7334.
Ng, D. et al. (2009) PloS Biol. 7:e41.
Xu, Y. et al. (2020) J. Cell. Mol. Med. 24:9114.
Neuropilin [NRP] And Tolloid [TLL]-like 1
Entrez Gene IDs
81832 (Human); 246317 (Mouse); 307206 (Rat)
BCTL1, BTCL1, BTCL1BCTL1neuropilin and tolloid-like protein 1, Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor classA domains protein 1, FLJ41325, NETO1, neuropilin (NRP) and tolloid (TLL)-like 1
Citations for Recombinant Human NETO1 His-tag Protein, CF
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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