Product Specifications for Recombinant Human Myocilin His-tag Protein, CF
Human embryonic kidney cell, HEK293-derived human Myocilin protein Leu215-Met504, with a C-terminal 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
28-35 kDa, reducing conditions
Measured by the ability of the immobilized protein to support the adhesion of AGS human Gastric Adenocarcinoma cells. The ED50 for this effect is 0.3-3 μg/mL.
Scientific Data Examples for Recombinant Human Myocilin His-tag Protein, CF
Recombinant Human Myocilin His-tag Protein Bioactivity
Immobilized Recombinant Human Myocilin supporst the adhesion of AGS human Gastric Adenocarcinoma cells. The ED50 for this effect is 0.3-3 μg/mL.
Recombinant Human Myocilin His-tag Protein SDS-PAGE
2 μg/lane of Recombinant Human Myocilin His-tag (Catalog# 3446-MY) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 28-35 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 500 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Myocilin is a secreted glycoprotein that belongs to the family of olfactomedin-related proteins (1). Mature human Myocilin is synthesized as a 472 amino acid (aa) precursor that can be cleaved into a 194 aa N-terminal fragment containing leucine zipper motifs within two coil–coil domains and a 335 aa C-terminal fragment containing an olfactomedin (OLF) domain (2). The human OLF-domain shares 87% aa sequence identity with the OLF-domain in mouse and rat. The Myocilin gene is expressed in the human eye compartments. When it is secreted, it can be detected in the cornea, trabecular meshwork, aqueous humor, iris, ciliary body, choroid sclera, retina and the axons of optic nerve ganglion cells (3). Mutations of the MYOC gene are associated with primary open angle glaucoma (POAG), which is a complex disorder with a major heritable component (4). Myocilin interacts with itself to form dimers or multimers (2, 5-8), flotilin-1, optimedin, fibronectin, and fibrilin-1 as well as hevin and SPARC (2, 8-10). Myocilin stimulates cell migration that involves the activation of intergrin focal adhesion kinase (FAK)-serin/threonine kinase (AKT) signaling pathway (11). Myocilin binds specifically to the Heparin II domain of fibronectin (12).
Zeng, L.C. et al. (2005) FEBS Lett. 579:5443.
Resch, Z.T. and Fautsch M.P. (2009) Exp Eye Res. 88:704.
Karali, A. et al. (2000) Invest. Ophthalmol. Vis. Sci. 41:729.
Ikezoe, T. et al. (2003) Int J Mol Med. 12:259.
Aroca-Aguilar, J.D. et al. (2013) PLoS One 8:e54385.
Tamm, E.R. (2002) Prog Retin Eye Res 21:395.
Fautsch, M.P. et al. (2006) Exp Eye Res 82:1046.
Aroca-Aguilar, J.D. et al. (2011) Invest Ophthalmol Vis Sci 52:179.
Wentz-Hunter, K. et al. (2002) Invest Ophthalmol Vis Sci 43:176.
Ueda, J. et al. (2000) J Histochem Cytochem 48:1321.
Kwon, H.S. and Tomarev, S.I. (2011) J Cell Physiol 226:3392.
Filla, M.S. et al. (2002) Invest Ophthalmol Visual Sci. 43:151.
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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