Recombinant Human Myeloperoxidase Protein, CF

Name: Recombinant Human Myeloperoxidase Protein, CF
Brand: R&D Systems
SKU: 3174-MP
Rating: 5 (2 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 3174-MP

Citations (8)

(2)

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Key Product Details

Source:	NS0
Accession #:	P05164
Applications:	Enzyme Activity

View Full Technical Details for Recombinant Human Myeloperoxidase Protein, CF

Product Specifications for Recombinant Human Myeloperoxidase Protein, CF

Source	Mouse myeloma cell line, NS0-derived human Myeloperoxidase/MPO protein Ala49-Ser745, with a C-terminal 10-His tag
Purity	>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ala49
Predicted Molecular Mass	80 kDa
SDS-PAGE	80-90 kDa, reducing conditions
Activity	Measured by its ability to oxidize guaiacol in the presence of hydrogen peroxide. Capeillere-Blandin, C. (1998) Biochem J. 336 :395. The specific activity is >50,000 pmol/min/µg, as measured under the described conditions.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3174-MP

Formulation	Lyophilized from a 0.2 μm filtered solution in Tris and NaCl.
Reconstitution	Reconstitute at 0.5-1 mg/mL in sterile, deionized water. Reconstitution Buffer Available: Size / Price Qty Loading... RB07:
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Myeloperoxidase/MPO

Myeloperoxidase (MPO) is a heme-containing enzyme belonging to the XPO subfamily of peroxidases. It is an abundant neutrophil and monocyte glycoprotein that catalyzes the hydrogen peroxide-dependent conversion of chloride, bromide, and iodide to multiple reactive species (1). Post-translational processing of MPO involves the insertion of a heme moiety and the proteolytic removal of both a propeptide and a 6 aa internal peptide (2). This results in a disulfide-linked dimer composed of a 60 kDa heavy and 12 kDa light chain that associate into a 150 kDa enzymatically active tetramer. The tetramer contains two heme groups and one disulfide bond between the heavy chains (2). Alternate splicing generates two additional isoforms of MPO, one with a 32 aa insertion in the light chain, and another with a deletion of the signal sequence and part of the propeptide (3). Human and mouse MPO share 87% aa sequence identity. MPO activity results in protein nitrosylation and the formation of 3-chlorotyrosine and dityrosine crosslinks (4‑6). Modification of ApoB100, as well as the lipid and cholesterol components of LDL and HDL, promotes the development of atherosclerosis (5, 7‑9). MPO is also associated with a variety of other diseases (1), and inhibits vasodilation in inflammation by depleting the levels of NO (10). Serum albumin functions as a carrier protein during MPO movement to the basolateral side of epithelial cells (11). MPO is stored in neutrophil azurophilic granules. Upon cellular activation, it is deposited into pathogen-containing phagosomes (2). While mice lacking MPO are impaired in clearing select microbial infections, MPO deficiency in humans does not necessarily result in heightened susceptibility to infections (12, 13).

Item	Value
References	Klebanoff, S.J. (2005) J. Leukoc. Biol. 77:598. Hansson, M. et al. (2006) Arch. Biochem. Biophys. 445:214. Hashinaka, K. et al. (1988) Biochemistry 27:5906. van Dalen, C.J. et al. (2000) J. Biol. Chem. 275:11638. Hazen, S.L. and J.W. Heinecke (1997) J. Clin. Invest. 99:2075. Heinecke, J.W. et al. (1993) J. Clin. Invest. 91:2866. Podrez, E.A. et al. (1999) J. Clin. Invest. 103:1547. Bergt, C. et al. (2004) Proc. Natl. Acad. Sci. 101:13032. Hazen, S.L. et al. (1996) J. Biol. Chem. 271:23080. Eiserich, J.P. et al. (2002) Science 296:2391. Tiruppathi, C. et al. (2004) Proc. Natl. Acad. Sci. 101:7699. Aratani Y. et al. (2000) J. Infect. Dis. 182:1276. Kutter, D. (1998) J. Mol. Med. 76:669.
Entrez Gene IDs	4353 (Human); 17523 (Mouse); 303413 (Rat)
Alternate Names	EC 1.11.1, EC 1.11.1.7, MPO, Myeloperoxidase

Citations for Recombinant Human Myeloperoxidase Protein, CF (8)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Myeloperoxidase Protein, CF include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

M Archer et al. (2022), Immune Regulation of Mammary Fibroblasts and the Impact of Mammographic Density Journal of Clinical Medicine, 11(3).
PMID: 35160252
Species: N/A
Sample Types: DNA
Applications: Bioassay

AL Rozelle et al. (2021), DNA interstrand cross-links induced by the major oxidative adenine lesion 7,8-dihydro-8-oxoadenine Nature Communications, 12(1):1897.
PMID: 33772030
Species: Human
Sample Types: Reference Standard
Applications: Bioassay

EJ Tóth et al. (2020), Response of Human Neutrophil Granulocytes to the Hyphae of the Emerging Fungal Pathogen Curvularia lunata Pathogens, 9(3).
PMID: 32245253
Species: N/A
Sample Types: Recombinant Protein
Applications: Bioassay

NWM de Jong et al. (2018), A Structurally Dynamic N-terminal Region Drives Function of the Staphylococcal Peroxidase Inhibitor (SPIN) J. Biol. Chem., 0(0).
PMID: 29306874
Species: Bacteria - Staphylococcus aureus
Sample Types: Recombinant Protein
Applications: Characterization

NT Ploscariu et al. (2018), Identification and structural characterization of a novel myeloperoxidase inhibitor from Staphylococcus delphini Arch. Biochem. Biophys., 645(0):1-11.
PMID: 29524428
Species: Bacteria - Staphylococcus aureus
Sample Types: Cell Culture Supernates
Applications: Bioassay

NWM de Jong et al. (2017), Immune evasion by a staphylococcal inhibitor of myeloperoxidase Proc. Natl. Acad. Sci. U.S.A., 0(0).
PMID: 28808028
Species: Human
Sample Types: Protein
Applications: Enzyme Assay

(2016), Gut Microbiota Conversion of Dietary Ellagic Acid into Bioactive Phytoceutical Urolithin A Inhibits Heme Peroxidases PLoS ONE, 11(6):e0156811.
PMID: 27254317
Species: N/A
Sample Types: N/A
Applications: Western Blot

Huang S et al. (2014), A novel zebrafish model to provide mechanistic insights into the inflammatory events in carrageenan-induced abdominal edema. PLoS ONE, 9(8):e104414.
PMID: 25141004

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Customer Reviews for Recombinant Human Myeloperoxidase Protein, CF (2)

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Recombinant Human Myeloperoxidase Protein, CF

Name: Anonymous

Application: Immunoassay Standard

Verified Customer | Posted 11/07/2018

The protein was used as a calibrator in an in vitro immunoassay.
Recombinant Human Myeloperoxidase Protein, CF

Name: Vishal Singh

Application: Use as standard in in vitro MPO activity assays.

Reason for Rating: Very stable protein

Verified Customer | Posted 05/23/2016

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Product Documents for Recombinant Human Myeloperoxidase Protein, CF

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Recombinant Human Myeloperoxidase Protein, CF

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Product Specifications for Recombinant Human Myeloperoxidase Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: Myeloperoxidase/MPO

Citations for Recombinant Human Myeloperoxidase Protein, CF (8)

Customer Reviews for Recombinant Human Myeloperoxidase Protein, CF (2)

Customer Images

Recombinant Human Myeloperoxidase Protein, CF

Recombinant Human Myeloperoxidase Protein, CF

Product Documents for Recombinant Human Myeloperoxidase Protein, CF

Product Datasheets

COA

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