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Recombinant Human MICB His-tag Protein, CF

Catalog # 10431-MB | R&D Systems, Inc. a Bio-Techne Brand
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10431-MB-050

Key Product Details

Source

CHO

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human MICB protein
Ala23-Thr308, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala23 & Glu24

Predicted Molecular Mass

33 kDa

SDS-PAGE

35-55 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human NKG2D/CD314 Fc Chimera (Catalog # 1299-NK) is immobilized at 0.125 μg/mL, 100 μL/well, the concentration of Recombinant Human MICB His-tag (Catalog # 10431-MB) that produces 50% of the optimal binding response is approximately 80-400 ng/mL.

Scientific Data Images for Recombinant Human MICB His-tag Protein, CF

Recombinant Human MICB His-tag Protein Binding Activity

Recombinant Human MICB His-tag Protein Binding Activity

When Recombinant Human NKG2D/CD314 Fc Chimera (Catalog # 1299-NK) is immobilized at 0.125 µg/mL, 100 µL/mL, the concentration of Recombinant Human MICB His-tag (Catalog # 10431-MB) produces 50% of the optimal binding response is approximately 80-400 ng/mL.
Recombinant Human MICB His-tag Protein SDS-PAGE

Recombinant Human MICB His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human MICB His-tag Protein (Catalog # 10431-MB) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands 35-55 kDa.

Formulation, Preparation and Storage

10431-MB
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MICB

Major Histocompatibility Complex Class I Chain-related Gene B (MICB) is a transmembrane glycoprotein that functions as a ligand for human Natural-Killer Group 2 Member D (NKG2D) (1). MICB, along with the highly related MICA, are members of the non-classical MHC class I family. Similar to classical MHC class I proteins, MICB contains three Ig-like domains ( alpha1, alpha2, and alpha3) in the extracellular domain (ECD), a transmembrane segment, and a carboxy-terminal cytoplasmic tail (2). The genes encoding MICA and MICB are found within the MHC on the short arm of human chromosome 6 and occur in most mammalian species but are absent from mouse and rat (2-4). Despite structural similarities, MICA/B only share ~27% amino acid (aa) identity with human MHC class I proteins (3). Both MICA and MICB display a significant degree of polymorphism within the ECD and this enables them to be reconfigured and bind with NKG2D rather than binding with beta2-microglobulin (4, 5). Recognition of MICA/B by NKG2D results in the activation of cytolytic activity and/or cytokine production on NK cells, NKT cells, gamma delta T cells, and CD8+ alpha beta T cells (6, 7). MICA/B recognition is involved in tumor surveillance, viral infections, and autoimmune diseases. The release of soluble forms of MICA/B from tumors down-regulates NKG2D surface expression on effector cells resulting in the impairment of anti-tumor immune response (2, 8-11). Inhibition of MICA/B shedding through targeted antibodies allows anti-tumor immunity to be retained (12).

References

  1. Huang, C. et al. (2018) Sci. Rep. 8:15821.
  2. Bahram, S. et al. (1994) Proc. Natl. Acad. Sci. USA 91:6259.
  3. Cosman, D. et al. (2001) Immunity 14:123.
  4. Groh, V. et al. (2001) Nature Immunol. 2:255.
  5. Stephens, H. (2001) Trends Immunol. 22:378.
  6. Bauer, S. et al. (1999) Science 285:727.
  7. Kawabata, Y. et al. (2000). Human Immunology. 61:624.
  8. Groh, V. et al. (2002) Nature 419:734.
  9. Steinle, A. et al. (2001) Immunogenetics 53:279.
  10. Pende, D. et al. (2002) Cancer Res. 62:6178.
  11. Salih, H. et al. (2003) Blood 102:1389.
  12. Ferrari de Andrade et al. (2018) Science 359:1537.

Long Name

MHC Class I-related Protein B

Alternate Names

MHC class I chain-related protein B, MHC class I mic-B antigen, MHC class I polypeptide-related sequence B, MIC-B, PERB11.2MHC class I-like molecule PERB11.2-IMX, stress inducible class I homolog

Entrez Gene IDs

4277 (Human)

Gene Symbol

MICB

UniProt

Product Documents for Recombinant Human MICB His-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human MICB His-tag Protein, CF

For research use only

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