Product Specifications for Recombinant Human MFG-E8 Protein, CF
Mouse myeloma cell line, NS0-derived human MFG-E8 protein Leu24-Cys387, with a C-terminal 6-His tag
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
40-58 kDa, reducing conditions
Measured by the ability of the immobilized protein to support the adhesion of SVEC4‑10 mouse vascular endothelial cells. When 5 x 104 cells/well are added to Recombinant Human MFG‑E8 coated plates (10 µg/mL, 100 µL/well), approximately 45‑75% will adhere after 60 minutes at 37 °C.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in NaH2PO4 and NaCl.
Reconstitute at 100 μg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Milk Fat Globulin Protein E8 (MFG-E8), also known as Lactadherin, MP47, breast epithelial antigen BA46, and SED1, is a 66-75 kDa pleiotropic secreted glycoprotein that promotes mammary gland morphogenesis, angiogenesis, and tumor progression. MFG-E8 also plays an important role in tissue homeostasis and the prevention of inflammation (1). Human MGF-E8 contains one N-terminal EGF-like domain and two C‑terminal F5/8-type discoidin-like domains (2). It shares 63% and 61% aa sequence identity with comparable regions of mouse and rat MFG-E8, respectively. Shorter isoforms of human MFG-E8 may have N-terminal deletions (beginning near the end of the first discoidin-like domain), internal deletions (lacking either the EGF-like domain or the central region of the second discoidin-like domain), or C‑terminal deletions (truncated within the second discoidin-like domain) (3). A 50 aa internal proteolytic fragment of human MFG-E8 (known as Medin) is a major component of aortic medial amyloid deposits (4). MFG-E8 is released into the milk in complex with lipid-containing milk fat globules. It is also found in multiple other cell types including endothelial cells and smooth muscle cells of the vasculature, immature dendritic cells, at the acrosomal cap of testicular and epididymal sperm, and in epithelial cells of the endometrium (1). MFG-E8 binds to the Integrins alpha V beta 3 and alpha V beta 5 and potentiates the angiogenic action of VEGF through VEGF R2 (5, 6). It reduces inflammation and tissue damage in a variety of settings. MFG-E8 functions as a bridge between phosphatidylserine on apoptotic cells and Integrin alpha V beta 3 on phagocytes, leading to the clearance of apoptotic debris (7). It mediates the engulfment of apoptotic bodies in atherosclerotic plaques and prion-infected brain (8, 9) and of apoptotic B cells during germinal center reactions (10, 11). MFG-E8 also promotes the removal of excess Collagen in fibrotic lungs and the regeneration of damaged intestinal epithelia (12, 13). Its tissue-protective role impairs anti‑tumor immunity and chemotherapy-induced apoptosis (14). MFG-E8 in the breastmilk blocks rotavirus infection in nursing babies (15).
Raymond, A. et al. (2009) J. Cell. Biochem. 106:957.
Couto, J.R. et al. (1996) DNA Cell Biol. 15:281.
Yamaguchi, H. et al. (2010) Eur. J. Immunol. 40:1778.
Haggqvist, B. et al. (1999) Proc. Natl. Acad. Sci. USA 96:8669.
Silvestre, J.-S. et al. (2005) Nat. Med. 11:499.
Borges, E. et al. (2000) J. Biol. Chem. 275:39867.
Hanayama, R. et al. (2002) Nature 417:182.
Ait-Oufella, H. et al. (2007) Circulation 115:2168.
Kranich, J. et al. (2010) J. Exp. Med. 207:2271.
Hanayama, R. et al. (2004) Science 304:1147.
Kranich, J. et al. (2010) J. Exp. Med. 205:1293.
Atabai, K. et al. (2009) J. Clin. Invest. 119:3713.
Bu, H.-F. et al. (2007) J. Clin. Invest. 117:3673.
Jinushi, M. et al. (2009) J. Exp. Med. 206:1317.
Kvistgaard, A.S. et al. (2004) J. Dairy Sci. 87:4088.
Customer Reviews for Recombinant Human MFG-E8 Protein, CF (1)
5 out of 5
1 customer rating
Recombinant Human MFG-E8 Protein, CF
In vitro bioactivity in cell culture
Reason for Rating:
Bioactivity is reproducible, and endotoxin levels are consistently low as promised.
Verified Customer | Posted
We use MFG-E8 as a ligand for phagocytosis assays involving AVB5 integrin receptor. Our reading is whether or not we see lipofuscin buildup from phagocytic overload. With MFG-E8 at 1.5ug/mL, we see significantly more lipofuscin buildup than without the recombinant protein, as applied to primary cell culture.
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