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Recombinant Human MFAP3 Fc Chimera Protein, CF

Catalog # 10800-MF | R&D Systems, Inc. a Bio-Techne Brand
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10800-MF-050

Key Product Details

Source

HEK293

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human MFAP3 protein
Human MFAP3
(Leu22-Met147)
Accession # P55082.1
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu22

Predicted Molecular Mass

41 kDa

SDS-PAGE

57-67 kDa, under reducing conditions

Activity

Measured by its ability to inhibit neurite outgrowth of E16-E18 rat embryonic cortical neurons.
Recombinant Human MFAP3 Fc Chimera, immobilized at 2.5-5.0 μg/mL on a 96 well plate, is able to significantly inhibit neurite outgrowth.

Scientific Data Images for Recombinant Human MFAP3 Fc Chimera Protein, CF

Recombinant Human MFAP3 Fc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Human MFAP3 Fc Chimera (Catalog # 10800-MF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 57-67 kDa and 114-134 kDa, respectively.

Formulation, Preparation and Storage

10800-MF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 1 mg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MFAP3

Microfibril-associated glycoprotein 3 (MFAP3), is a member of the MFAP family which play a role in microfibril assembly, elastinogenesis, and tissue homeostasis. The family members were identified as potential components of fibrillin-containing microfibrils, but they do not share structural similarities (1). MFAP3 is found in ocular zonules. Human MFAP3 is a serine-rich acidic protein located in zonular fibers and consists of an extracellular domain (ECD) containing one Ig-like C2-type domain, a single transmembrane domain, and a cytoplasmic domain. Within the ECD, mature human MFAP3 shares 74% amino acid sequence identity with mouse and rat MFAP3. Among MFAP3-related pathways are degradation of the extracellular matrix and elastic fiber formation. MFAP is a candidate gene for heritable diseases affecting microfibrils (2).

References

  1. Gibson, M.A. Landes Bioscience https://www.ncbi.nlm.nih.gov/books/NBK6448/.
  2. Abrams W.R. (1995) Genomics 26:47.

Long Name

Microfibrillar-Associated Protein 3

Alternate Names

DKFZp586F0219, microfibril-associated glycoprotein 3, microfibrillar-associated protein 3

Entrez Gene IDs

4238 (Human); 216760 (Mouse); 287299 (Rat)

Gene Symbol

MFAP3

UniProt

Product Documents for Recombinant Human MFAP3 Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human MFAP3 Fc Chimera Protein, CF

For research use only

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