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Recombinant Human Mer Fc Chimera Protein, CF
Catalog # 891-MR | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Accession #
Source
Sf 21 (baculovirus)
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Binding Activity
Product Specifications
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Mer protein
| Human Mer (Arg26-Ala499) Accession # AAB60430.1 |
IEGRMD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus |
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Arg26
Predicted Molecular Mass
78 kDa (monomer)
SDS-PAGE
100-110 kDa, reducing conditions
Activity
Measured by its binding ability in a functional ELISA.
Immobilized Recombinant Human (rh) Mer Fc Chimera at 10 µg/mL (100 µL/well) can bind rhGas6 (Catalog # 885-GSB) with a linear range of 0.03-1 µg/mL.
Immobilized Recombinant Human (rh) Mer Fc Chimera at 10 µg/mL (100 µL/well) can bind rhGas6 (Catalog # 885-GSB) with a linear range of 0.03-1 µg/mL.
Formulation, Preparation and Storage
891-MR
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution |
Reconstitute at 100 μg/mL in sterile PBS.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Mer
Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken c-Eyk) constitute a receptor tyrosine kinase subfamily. The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. These receptors bind the vitamin K-dependent protein growth-arrest-specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration or the prevention of apoptosis. Recent studies suggest that this family of tyrosine kinase receptors may be involved in hematopoiesis, embryonic development, tumorigenesis and regulation of testicular functions.
References
- Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
- Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.
Long Name
Receptor Tyrosine Protein Kinase Mer
Alternate Names
c-Eyk, C-mer, MerTK
Gene Symbol
MERTK
UniProt
Product Documents for Recombinant Human Mer Fc Chimera Protein, CF
Product Specific Notices for Recombinant Human Mer Fc Chimera Protein, CF
For research use only
Citations for Recombinant Human Mer Fc Chimera Protein, CF (5)
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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Mer Fc Chimera Protein, CF include:
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Species: Human
Sample Types: Protein
Applications: SPRF Wanke et al. (2021-05-25), Ligand-dependent kinase activity of MERTK drives efferocytosis in human iPSC-derived macrophages Cell Death & Disease, 2021-012(6):538.
PMID: 34035216 -
Species: Human
Sample Types: Recombinant Protein
Applications: ImmunoprecipitationY Zhao et al. (2018-03-07), TREM2 Is a Receptor for ?-Amyloid that Mediates Microglial Function Neuron, 2018-097(5):1023-1031.e7.
PMID: 29518356 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayZizzo G et al. (2012-08-31), Efficient clearance of early apoptotic cells by human macrophages requires M2c polarization and MerTK induction. J Immunol, 2012-0189(7):3508-20.
PMID: 22942426 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayCerchia et al. (2012-08-21), Targeting Axl with an high-affinity inhibitory aptamer. Mol Ther, 2012-020(12):2291-303.
PMID: 22910292 -
Species: Human
Sample Types: Phospholipid Vesicles
Applications: BioassayUehara H et al. (2008-02-15), Auto-oxidation and oligomerization of protein S on the apoptotic cell surface is required for mer tyrosine kinase-mediated phagocytosis of apoptotic cells. J. Immunol., 2008-0180(4):2522-30.
PMID: 18250462
There are no citations that match your criteria.
Customer Reviews for Recombinant Human Mer Fc Chimera Protein, CF (3)
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 11/12/2020
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Application: Binding assay/Protein-protein interactionVerified Customer | Posted 04/03/2018
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Application: Immunoassay StandardVerified Customer | Posted 03/23/2018
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