Mouse myeloma cell line, NS0-derived human MEGF9 protein Ala36-Asn514, with a C-terminal 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
136-150 kDa, under reducing conditions
Measured by its ability to inhibit neurite outgrowth of E16-E18 rat embryonic cortical neurons. Recombinant Human MEGF9 His-tag, immobilized at 0.15 μg/mL on a 96 well plate, is able to significantly inhibit neurite outgrowth.
Recombinant Human MEGF9 His-tag Protein, CF Scientific Data Examples
Recombinant Human MEGF9 His-tag Protein SDS-PAGE
2 μg/lane of Recombinant Human MEGF9 His-tag (Catalog # 10514-ME) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 136-150 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 1 mg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
MEGF9 (Multiple EGF‑like domains protein 9; also EGF‑like protein 5) is a novel transmembrane glycoprotein with multiple EGF-like repeats. It is predominantly expressed in the developing and adult central nervous system and peripheral nervous system. Immunohistochemical studies showed that MEGF9 is expressed in Purkinje cells of the cerebellum and in glial cells of the peripheral nervous system. In non-neuronal tissues, MEGF9 is expressed in epidermal layer of skin, papillae of the tongue and epithelium of the gastrointestinal tract. MEGF9 is suggested to participate in cell motility, and its absence correlates with tumor cell migration. Mature human MEGF9 is 572 amino acids (aa) in length. It is a single span type I transmembrane protein that contains a 484 aa extracellular domain (ECD). The extracellular region possesses a lengthy Pro‑rich region (aa 55‑200). N-terminal region of MEGF9 includes several potential O-glycosylation sites followed by five EGF-like domains which are highly homologous to the short arms of laminins. Following one single pass transmembrane domain, a highly conserved short intracellular domain with potential phosphorylation sites is present (1, 2). Within the ECD, human MEGF9 shares 75% aa sequence identity with mouse and rat MEGF9.
Brandt-Bohne, U. et al. (2007) Biochem. J. 401:447.
Heller, B. et al. (2014) J. Cell. Biol. 204:1219.
Multiple EGF-like-domains 9
Entrez Gene IDs
1955 (Human); 230316 (Mouse); 313270 (Rat)
EGF-like protein 5, EGF-like-domain, multiple 5, EGFL5, EGFL5KIAA0818, Epidermal growth factor-like protein 5, MEGF9, Multiple EGF-like domains protein 9, multiple EGF-like-domains 9, multiple epidermal growth factor-like domains protein 9
Citations for Recombinant Human MEGF9 His-tag Protein, CF
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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