Recombinant Human MANF Protein, CF

Name: Recombinant Human MANF Protein, CF
Brand: R&D Systems
SKU: 3748-MN

Catalog # 3748-MN | R&D Systems, Inc. a Bio-Techne Brand

Citations (2)

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Key Product Details

Source:	NS0
Accession #:	ACE87065
Applications:	Bioactivity

View Full Technical Details for Recombinant Human MANF Protein, CF

Technical Data
Citations (2) / Reviews

Product Specifications for Recombinant Human MANF Protein, CF

Source	Mouse myeloma cell line, NS0-derived human MANF protein Leu22-Leu179 with a C-terminal 6-His tag
Purity	>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Leu22
Predicted Molecular Mass	19.0 kDa
SDS-PAGE	19.0 kDa
Activity	Measured by its ability to support the survival and stimulate neurite outgrowth of rat embryonic cortical neurons. The ED₅₀ for this effect is 0.7-2.8 μg/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3748-MN

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 200 μg/mL in PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: MANF

Mesencephalic astrocyte-derived neurotrophic factor (MANF), also known as arginine-rich, mutated in early stage tumors (ARMET) and arginine-rich protein (ARP), is a 20 kDa member of the ARMET family of proteins (1). The name ARMET comes from the fact that the protein was initially thought to be 50 aa longer at the N-terminus and to contain an arginine-rich region (2-5). The presence of a specific mutation changing the previously numbered codon 50 from ATG to AGG, or deletion of that codon, has been reported in a variety of solid tumors (2-4). Human MANF is synthesized as a 179 amino acid (aa) precursor that contains a 21 aa signal sequence and a 158 aa mature chain. Mature human MANF is 99%, 98% and 96% aa identical to mature rat, mouse and bovine MANF, respectively. MANF is localized to the endoplasmic reticulum (ER) and Golgi apparatus, and is also secreted (5). In the CNS, MANF selectively protects nigral dopaminergic neurons, versus GABAergic or serotonergic neurons, which suggests that MANF may be indicated for the treatment of Parkinson’s disease (1). MANF is also one of the 12 commonly unfolded protein response (UPR)-up-regulated genes (5). One study showed that MANF plays an important role in protecting cells against tunicamycin and thapsigargin-induced cell death (5). Loss of MANF renders cells more susceptible to those drugs, but also increases cell proliferation and decreases cell size (5). Another study showed that MANF is an endoplasmic reticulum stress response (ERSR) gene in the heart that can be induced and secreted in response to ER stresses, including ischemia, and that extracellular MANF may protect cardiac myocytes in an autocrine and paracrine manner (6).

Item	Value
References	Petrova, P.S. et al. (2003) J. Mol. Neurosci. 20:173. Shridhar, V. et al. (1996) Oncogene 12:1931. Shridhar, R. et al. (1996) Cancer Res. 56:5576. Shridhar, V. et al. (1997) Oncogene 14:2213. Apostolou, A. et al. (2008) Exp. Cell Res. 314:2454. Tadimalla, A. et al. (2008) Circ. Res. 103:1249.
Long Name	Mesencephalic Astrocyte-derived Neurotrphic Factor
Entrez Gene IDs	7873 (Human); 74840 (Mouse); 315989 (Rat)
Alternate Names	ARMET, ARP, Arginine-Rich Protein, MANF, Protein ARMET, arginine-rich, mutated in early stage tumors, mesencephalic astrocyte-derived neurotrophic factor

Citations for Recombinant Human MANF Protein, CF (2)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human MANF Protein, CF include:

Species: Human, Mouse
Sample Types: Whole Cells
Applications: Bioassay

J Mahadevan et al. (2020-05-04), A soluble endoplasmic reticulum factor as regenerative therapy for Wolfram syndrome Lab. Invest., 2020-00(0).
PMID: 32366942
Species: Transgenic Mouse
Sample Types: Whole Cells
Applications: Bioassay

SJ Park et al. (2019-06-24), Discovery of endoplasmic reticulum calcium stabilizers to rescue ER-stressed podocytes in nephrotic syndrome Proc. Natl. Acad. Sci. U.S.A., 2019-00(0).
PMID: 31235574

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