Human embryonic kidney cell, HEK293-derived human MANBA/Beta-Mannosidase protein Ala19-Tyr879 & Glu20 - Ser878, with C-terminal 6-His tag
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Ala19 & Glu20
Predicted Molecular Mass
110 kDa, under reducing conditions
Measured by its ability to hydrolyze 4-methylumbelliferyl Beta-D-mannopyranoside. The specific activity is >100 pmol/min/μg, as measured under the described conditions.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Supplied as a 0.2 μm filtered solution in Tris and NaCl.
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 °C as supplied.
3 months, -20 to -70 °C under sterile conditions after opening.
MANBA is a lysosomal beta -mannosidase in the degradation pathway of N-glycans and catalyzes the removal of the innermost beta (1-4)-linked mannose residue (1). Mutations of MANBA can lead to cellular storage and secretion in the urine of the disaccharide, Man( beta 1–4)GlcNAc (2), and the symptoms of lysosomal storage disease (LSD) beta -mannosidosis, associated with mental retardation, behavioral problems including hyperactivity and aggressiveness, developmental delay, frequent infections, and hearing loss (3). Angiokeratoma corporis diffusum is another symptom of beta ‐mannosidosis that is characterized by involuntary eye movements (4) and can be found in other LSDs such as Fabry disease, beta ‐galactosidosis, fucosidosis, aspartylglucosaminuria, and galactosialidosis (5). LSDs are progressive disorders and are among the most common genetic disorders affecting children (6). Patients with LSDs are usually treated with enzyme replacement therapy (ERT) in which recombinant enzymes are administered to them for direct uptake into the lysosome (7). MAMBA belongs to the glycosyl hydrolase (GH) 2 family, in which enzymes are classified on the basis of sequence similarity (8). The only other human GH2 enzyme is beta ‐glucuronidase, deficiency of which leads to Sly syndrome (9). GH2 family members are characterized by a catalytic TIM barrel (10), in which the catalytic acid-base and nucleophile residues lie on beta ‐strands 4 and 7, respectively. Human beta ‐mannosidase is sensitive to pH with optima between 4.5 and 5.0 (11).
Alkhayat, A. H. et al. (1998) Hum Molec Genet 7:75.
Hokke, C.H. et al. (1990). J Inherit Metab Dis 13:273.
Wenger, D.A. et al. (1986). N Engl J Med 315:1201.
Gytz, H. et al. (2019) FEBS J. 286:1319.
Rodriguez‐Serna, M. et al. (1996) Arch Dermatol 132:1219.
Hawkins‐Salsbury, J.A. et al. (2011) Hum Mol Genet 20:R54.
Wyatt, K. et al. (2012) Health Technol Assess 16:1.
Cantarel, B.L. et al. (2009). Nucleic Acids Res 37:D233.
Sly, W.S. et al. (1973). J. Pediatr. 82:249.
Henrissat, B. et al. (1995). Proc Natl Acad Sci USA 92:7090.
Percheron, F. et al. (1992). Biochimie 74:5.
Entrez Gene IDs
4126 (Human); 110173 (Mouse)
EC 22.214.171.124, Lysosomal beta A mannosidase, MANB1, MANB1mannanase, MANBA, Mannanase, Mannase, beta-mannosidase, mannosidase, beta A, lysosomal
Citations for Recombinant Human MANBA/Beta-Mannosidase His-tag Protein, CF
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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