Recombinant Human LYPD6 Fc Chimera Protein, CF

Name: Recombinant Human LYPD6 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 10073-LY

Catalog # 10073-LY

Product Datasheet / COA / SDS

Order Information

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Key Product Details

Source:	HEK293
Accession #:	Q86Y78-1
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human LYPD6 Fc Chimera Protein, CF

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human LYPD6 protein

Human LYPD6 (Ala23-His152) Accession # Q86Y78-1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Ala23

Predicted Molecular Mass

41 kDa

SDS-PAGE

58-66 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human LYPD6 Fc Chimera is immobilized at 2 µg/mL (100 µL/well), Biotinylated Recombinant Human Frizzled-8 Fc Chimera binds with an ED₅₀ of 2-12 μg/mL.

Recombinant Human LYPD6 Fc Chimera Protein, CF Scientific Data Examples

	Recombinant Human LYPD6 Fc Chimera Protein Binding Activity When Recombinant Human LYPD6 Fc Chimera (Catalog # 10073-LY) is coated at 2 μg/mL, 100 μL/well, Biotinylated Recombinant Human Frizzled-8 Fc Chimera binds with an ED₅₀ of 2-12 μg/mL.
	Recombinant Human LYPD6 Fc Chimera Protein SDS-PAGE 2 μg/lane of Recombinant Human LYPD6 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 58-66 kDa and 120 -130 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10073-LY

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Desired Reconstitution Concentration

Background: LYPD6

LYPD6 (Ly6/PLAUR domain-containing protein 6) is a member of the Ly6/uPar family of proteins that are either secreted or GPI-anchored to the cell surface. Members of this protein family include immune regulatory molecules like CD59, the urokinase plasminogen activator receptor (uPar) and molecules that regulate cell proliferation, migration and adhesion (1-3). Ly6/uPar proteins are characterized by the presence of variable loop regions which confer the functional diversity of this protein family (4). Human LYPD6 is synthesized as a 171 aa protein that includes a 22 aa signal peptide and a 149 aa LYPD6 chain. Within the main chain region, human LYPD6 shares 95% sequence identity with mouse LYPD6. LYPD6 is an important regulator of embryogenesis in zebrafish through its enhancement of Wnt/ beta -catenin signaling (5). It is widely expressed in human tissues with higher expression level in the brain and the heart (5-7). Studies have shown LYPD6 can interact with Frizzled-8 and LRP6, and it is required for Wnt-8-mediated mesoderm and neuroectoderm patterning (5). Also, it has been shown LYPD6 can interact with several nicotinic acetylcholine receptors (nAChRs) in the human brain (7). Soluble recombinant LYPD-6 can inhibit nicotine-induced phosphorylation of ERK in PC12 cells and attenuate nicotine-induced hippocampal inward currents in rat brain slices (7).

Item	Value
References	Kong, B.H. and J.H. Park (2012) BMB Rep. 45:595. Bamezai, A. (2004) Arch. Immunol. Ther. Exp. (Warsz.) 52:255. Gumley, T.P. et al. (1995) Immunol. Cell. Biol. 73:277. Vasilyeva, N.A. et al. (2017) Biochemistry (Mosc.) 82:1702. Özhan, G. et al. (2013) Dev Cell. 26(4):331. Zhang, Y. et al. (2010) Mol Biol Rep 37:2055. Arvaniti, M. et al. (2016) J. Neurochem. 138:806.
Long Name	Ly6/PLAUR Domain-containing Protein 6
Entrez Gene IDs	130574 (Human); 320343 (Mouse); 679564 (Rat)
Alternate Names	LY6/PLAUR domain containing 6, LYPD6, UNQ3023/PRO9821

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