Recombinant Human Lymphotoxin-alpha/TNF-beta Protein

Name: Recombinant Human Lymphotoxin-alpha/TNF-beta Protein
Brand: R&D Systems
SKU: 211-TBB
Rating: 5 (1 reviews)

Catalog # 211-TBB | R&D Systems, Inc. a Bio-Techne Brand

(1)

Citations (8)

Product Datasheet / COA / SDS

Catalog #

Availability

Size / Price

Qty

Setting the standard in quality research reagents

Key Product Details

Source:	E. coli
Accession #:	P01374
Applications:	Bioactivity

View Full Technical Details for Recombinant Human Lymphotoxin-alpha/TNF-beta Protein

Product Specifications for Recombinant Human Lymphotoxin-alpha/TNF-beta Protein

Source	E. coli-derived human Lymphotoxin-alpha/TNF-beta protein Leu35-Leu205, with an N-terminal Met
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Met
Predicted Molecular Mass	19 kDa
SDS-PAGE	17-19 kDa, reducing conditions
Activity	Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED₅₀ for this effect is 4-20 pg/mL.

Scientific Data Examples for Recombinant Human Lymphotoxin-alpha/TNF-beta Protein

	Recombinant Human Lymphotoxin-alpha/TNF-beta Protein Bioactivity Recombinant Human Lymphotoxin-alpha /TNF-beta (Catalog # 211-TBB) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED₅₀ for this effect is 4-20 pg/mL.
	Recombinant Human Lymphotoxin-alpha/TNF-beta Protein SDS-PAGE 1 µg/lane of Recombinant Human Lymphotoxin-alpha /TNF-beta was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 19 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

211-TBB

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

211-TBB/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Lymphotoxin-alpha/TNF-beta

Lymphotoxin-alpha (LT-alpha ), also known as Tumor Necrosis Factor-beta (TNF-beta ), is a member of the TNF Superfamily. Human LT-alpha /TNF-beta is a 22 kDa protein that shares 73% amino acid sequence identity with mouse and rat LT-alpha /TNF-beta (1-3). Secreted LT-alpha forms homotrimers that bind and activate TNF RI/TNFRSF1A, TNF RII/TNFRSF1B, and HVEM/TNFRSF14 (4). LT-alpha /TNF-beta also forms heterotrimers with plasma membrane-localized LT-beta to bind and activate the LT-beta R/TNFRSF3 (4). In addition to its cytotoxic action on tumor cells, LT-alpha /TNF-beta mediates lymph node development, inflammation, and immune function (5-8). LT-alpha /TNF-beta is expressed in activated T- and B lymphocytes and contributes to autoimmune disease (9, 10).

Item	Value
References	Nedwin, G.E. et al. (1985) J. Cell. Biochem. 29:171. Gray, P.W. et al. (1984) Nature 312:721. Kobayashi, Y. et al. (1986) J. Biochem. 100:727. Warzocha, K. et al. (1995) Eur. Cytokine Netw. 6:83. McCarthy, D.D. et al.(2006) Immunol. Res. 35:41. Drutskaya, M.S. et al. (2010) IUBMB Life 62:283. De Togni, P. et al. (1994) Science 264:703. Seleznik, G.M. et al. (2014) Cytokine Growth Factor Rev. 25:125. Mapara, M.Y. et al. (1994) Int. J. Cancer 58:248. Chiang, E.Y. et al. (2009) Nat. Med. 15:766.
Long Name	Tumor Necrosis Factor beta
Entrez Gene IDs	4049 (Human); 16992 (Mouse)
Alternate Names	LT, LT-alpha, LTA, Lymphotoxin alpha, Lymphotoxin-alpha, Lymphotoxinalpha, TNF-beta, TNFBlymphotoxin-alpha, TNFSF1, TNFSF1B, TNFSF1TNF-beta, Tumor necrosis factor ligand superfamily member 1, lymphotoxin alpha (TNF superfamily, member 1), tnfb, tumor necrosis factor beta

Citations for Recombinant Human Lymphotoxin-alpha/TNF-beta Protein (8)

Showing 1 - 8 of 8 citations Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Lymphotoxin-alpha/TNF-beta Protein include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

M Fellermann et al. (2020-09-01), Clostridial C3 Toxins Enter and Intoxicate Human Dendritic Cells Toxins (Basel), 2020-012(9).
PMID: 32883045
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

TK Darling et al. (2020-01-30), EphA2 contributes to disruption of the blood-brain barrier in cerebral malaria PLoS Pathog., 2020-016(1):e1008261.
PMID: 31999807
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

JG Chen et al. (2018-03-01), BTKCys481Serdrives ibrutinib resistance via ERK1/2, and protects BTKWild-TypeMYD88 mutated cells by a paracrine mechanism Blood, 2018-00(0).
PMID: 29496671
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Sarhan D et al. (2015-04-17), Dendritic cell regulation of NK-cell responses involves lymphotoxin-alpha, IL-12, and TGF-beta. Eur J Immunol, 2015-045(6):1783-93.
PMID: 25773885
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Steeland S et al. (2014-12-23), Generation and characterization of small single domain antibodies inhibiting human tumor necrosis factor receptor 1. J Biol Chem, 2014-1290(7):4022-37.
PMID: 25538244
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Yaden B et al. (2014-04-01), Inhibition of activin A ameliorates skeletal muscle injury and rescues contractile properties by inducing efficient remodeling in female mice. Am J Pathol, 2014-0184(4):1152-66.
PMID: 24655377
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Vang et al. (2013-12-15), Regulatory T-cells and cAMP suppress effector T-cells independently of PKA-CREM/ICER: a potential role for Epac. Biochem J, 2013-1456(3):463-73.
PMID: 24007532
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Johnson LA et al. (2006-11-20), An inflammation-induced mechanism for leukocyte transmigration across lymphatic vessel endothelium. J. Exp. Med., 2006-1203(12):2763-77.
PMID: 17116732

There are no citations that match your criteria.

Showing 1 - 8 of 8 citations Showing All

Customer Reviews for Recombinant Human Lymphotoxin-alpha/TNF-beta Protein (1)

5 out of 5

1 customer rating

5 stars

100%

4 stars

3 stars

2 stars

1 star

Customer Images

Showing 1 - 1 of 1 review Showing All

Filter By:

Recombinant Human Lymphotoxin-alpha/TNF-beta Protein

Name: Anonymous

Application: Immunoassay Standard

Verified Customer | Posted 11/06/2018

The recombinant TNFb was used in an ELISA with a sensitivity of 6pg/ml.

Recombinant Human Lymphotoxin-alpha/TNF-beta Protein

Catalog # 211-TBB | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Customers also Viewed

Product Specifications for Recombinant Human Lymphotoxin-alpha/TNF-beta Protein

Scientific Data Examples for Recombinant Human Lymphotoxin-alpha/TNF-beta Protein

Formulation, Preparation and Storage

Carrier Free

211-TBB

Carrier

211-TBB/CF

Carrier Free

Reconstitution Calculator

Background: Lymphotoxin-alpha/TNF-beta

Citations for Recombinant Human Lymphotoxin-alpha/TNF-beta Protein (8)

Customer Reviews for Recombinant Human Lymphotoxin-alpha/TNF-beta Protein (1)

Customer Images

Recombinant Human Lymphotoxin-alpha/TNF-beta Protein

Product Documents for Recombinant Human Lymphotoxin-alpha/TNF-beta Protein

Product Datasheets

COA

Certificate of Analysis

SDS

Bio-Techne Brands

Stay Connected