Source	Chinese Hamster Ovary cell line, CHO-derived human Ly6H protein Leu26-Gly115, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Leu26
Predicted Molecular Mass	10.7 kDa
SDS-PAGE	13-25 kDa, under reducing conditions
Activity	Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells. The ED₅₀ for this effect is 0.75-7.5 μg/mL

Recombinant Human Ly6H His-tag Protein, CF Scientific Data Examples

	Recombinant Human Ly6H His-tag Protein SDS-PAGE 2 μg/lane of Recombinant Human Ly6H His-tag Protein (Catalog # 10373-L6) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 13-25 kDa.
	Recombinant Human Ly6H His-tag Protein Bioactivity Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion by human T cells. The ED₅₀ for this effect is 0.75-7.5 μg/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10373-L6

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ly6H

Lymphocyte Antigen 6 Family Member H (Ly6H), also known as NMLy6, is a member of the Ly6/urokinase-type plasminogen activator receptor (uPAR) superfamily of proteins. LY6 proteins are primarily glycosylphosphatidylinositol (GPI)-anchored cell surface glycoproteins characterized by the presence of an LU domain. The LU domain is an ~80 amino acid (aa) region with a conserved disulfide bridge arrangement resulting in a three-fingered structural motif (1). The mature extracellular domain of human Ly6H shares 92% aa sequence identity with mouse Ly6H. Ly6H is predominantly expressed in normal brain tissue and can modulate alpha 7 nicotinic acetylcholine receptor ( alpha 7 nAChR) signaling (2, 3). The alpha 7 nAChR signaling is associated with several conditions, such as nicotine addiction, lung and liver cancer, preeclampsia, schizophrenia, and Alzheimer's disease (2, 3). The alpha 7-subunits are controlled by prototoxins in the Ly6 family. Specifically, Ly6H reduces cell-surface expression and calcium signaling by alpha 7 nAChRs, thus suppressing alpha 7 nAChR function (2, 3). Ly6H expression is increased in tumors associated with several cancer types (4). The increase in expression is associated with poor outcome on patient survival in breast, lung, ovarian, gastric, and colorectal cancer (4).

Item	Value
References	Upadhyay, G. et al. (2019) Front Immunol. 10:819. Puddifoot, C. A. et al. (2015) J Neurosci. 35:3420. Govind, A. et al. (2012) J Neurosci. 32:2227. Luo, L. et al. (2016) Oncotarget. 7:11165.
Long Name	Lymphocyte Antigen 6 Family Member H
Entrez Gene IDs	4062 (Human); 23934 (Mouse)
Alternate Names	Ly-6H, Ly6H, NMLy6

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Recombinant Human Ly6H His-tag Protein, CF

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