Recombinant Human Ly6H His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10373-L6

R&D Systems, part of Bio-Techne
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10373-L6-050
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Key Product Details

Source

CHO

Accession #

O94772.1

Conjugate

Unconjugated

Applications

Bioactivity

View all Ly6H Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Ly6H protein
Leu26-Gly115, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu26

Predicted Molecular Mass

10.7 kDa

SDS-PAGE

13-25 kDa, under reducing conditions

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells.
The ED50 for this effect is 0.75-7.5 μg/mL

Scientific Data Images for Recombinant Human Ly6H His-tag Protein, CF

Recombinant Human Ly6H His-tag Protein SDS-PAGE

Recombinant Human Ly6H His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human Ly6H His-tag Protein (Catalog # 10373-L6) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 13-25 kDa.
Recombinant Human Ly6H His-tag Protein Bioactivity

Recombinant Human Ly6H His-tag Protein Bioactivity

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion by human T cells. The ED50 for this effect is 0.75-7.5 μg/mL.

Formulation, Preparation and Storage

10373-L6
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ly6H

Lymphocyte Antigen 6 Family Member H (Ly6H), also known as NMLy6, is a member of the Ly6/urokinase-type plasminogen activator receptor (uPAR) superfamily of proteins. LY6 proteins are primarily glycosylphosphatidylinositol (GPI)-anchored cell surface glycoproteins characterized by the presence of an LU domain. The LU domain is an ~80 amino acid (aa) region with a conserved disulfide bridge arrangement resulting in a three-fingered structural motif (1). The mature extracellular domain of human Ly6H shares 92% aa sequence identity with mouse Ly6H. Ly6H is predominantly expressed in normal brain tissue and can modulate alpha7 nicotinic acetylcholine receptor ( alpha7 nAChR) signaling (2, 3). The alpha7 nAChR signaling is associated with several conditions, such as nicotine addiction, lung and liver cancer, preeclampsia, schizophrenia, and Alzheimer's disease (2, 3). The alpha7-subunits are controlled by prototoxins in the Ly6 family. Specifically, Ly6H reduces cell-surface expression and calcium signaling by alpha7 nAChRs, thus suppressing alpha7 nAChR function (2, 3). Ly6H expression is increased in tumors associated with several cancer types (4). The increase in expression is associated with poor outcome on patient survival in breast, lung, ovarian, gastric, and colorectal cancer (4).

References

  1. Upadhyay, G. et al. (2019) Front Immunol. 10:819.
  2. Puddifoot, C. A. et al. (2015) J Neurosci. 35:3420.
  3. Govind, A. et al. (2012) J Neurosci. 32:2227.
  4. Luo, L. et al. (2016) Oncotarget. 7:11165.

Long Name

Lymphocyte Antigen 6 Family Member H

Alternate Names

Ly-6H, NMLy6

Entrez Gene IDs

4062 (Human); 23934 (Mouse)

Gene Symbol

LY6H

UniProt

O94772.1

Additional Ly6H Products

Product Documents for Recombinant Human Ly6H His-tag Protein, CF

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Product Specific Notices for Recombinant Human Ly6H His-tag Protein, CF

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