Chinese Hamster Ovary cell line, CHO-derived human LRRN2 protein Ala19-Gly630, with a C-terminal 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Ala19 & Val20
Predicted Molecular Mass
73-81 kDa, under reducing conditions
Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Recombinant Human LRRN2, immobilized at 2.5 μg/mL on a 96 well plate, is able to significantly enhance neurite outgrowth.
Recombinant Human LRRN2 His-tag, CF Scientific Data Examples
Recombinant Human LRRN2 His-tag SDS-PAGE
2 μg/lane of Recombinant Human LRRN2 His-tag was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 73-81 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 500 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Leucine-rich repeat neuronal protein 2 (LRRN2) is a type I transmembrane protein containing leucine-rich repeats (1). Mature human LRRN-2 consists of a 612 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 62 aa cytoplasmic domain. Within the ECD, human LRRN-2 shares 92% with mouse and rat LRRN-2. LRRN-2 is expressed in neuronal tissue postsynaptic membranes (1, 2). It is involved in the development and maintenance of excitatory synapse in the vertebrate nervous system. LRRN-2 regulates surface expression of AMPA receptors and instructs the development of functional glutamate release sites (1, 2). LRRN-2 is up-regulated after NGF-induced differentiation and down-regulated after NGF depletion-induced apoptosis in mouse superior cervical ganglion cells (2).
Lauren, J. et al. (2003) Genomics 81:411.
Hamano, S. et al. (2004) Int. J. Oncol. 24:1457.
Leucine-rich Repeat Neuronal 2
Entrez Gene IDs
10446 (Human); 16980 (Mouse); 289020 (Rat)
FIGLER7, GAC1, GAC1LRRN5, Glioma amplified on chromosome 1 protein, LRANK1, LRRN2, LRRN5, Leucine-rich repeat neuronal protein 5, immunoglobulin and leucine rich repeat domain 7, leucine rich and ankyrin repeats 1, leucine rich repeat neuronal 2, leucine rich repeat neuronal 5, leucine-rich repeat neuronal protein 2
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