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Recombinant Human LRRC24 His-tag Protein, CF

Catalog # 10512-LR | R&D Systems, Inc. a Bio-Techne Brand
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10512-LR-050

Key Product Details

Source

CHO

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human LRRC24 protein
Cys23-Ser365, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Cys23

Predicted Molecular Mass

38 kDa

SDS-PAGE

45-55 kDa, under reducing conditions

Activity

Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons.
Recombinant Human LRRC24 His-tag, immobilized at 2.5 μg/mL on a 96 well plate, is able to significantly enhance neurite outgrowth.

Scientific Data Images for Recombinant Human LRRC24 His-tag Protein, CF

Recombinant Human LRRC24 His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human LRRC24 His-tag (Catalog # 10512-LR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 45-55 kDa.

Formulation, Preparation and Storage

10512-LR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LRRC24

Leucine-rich repeat-containing protein 24 (LRRC24) is type I transmembrane protein found within the large LRR superfamily. The LRR family is known to have diverse functions, including roles in innate immunity and nervous system development (1). The extracellular domain (ECD) of LRRC24 contains several LRR repeats along with an Ig-like C2-type region. The mature ECD of human LRRC24 shares 86% and 80% amino acid sequence identity with mouse and rat LRRC24, respectively. LRRC24 has been shown to interact with Robo2, which is important for axon guidance and cell migration (2). LRRC24 has been suggested to be a novel negative regulator of the ErbB family and suppress ErbB-driven tumor cell proliferation and motility (3).

References

  1. Ng, A.C.Y. et al. (2011) PNAS 108:4631.
  2. Söllner, C. and Wright, G.J. (2009) Genome Biol. 10:R99.
  3. Andrews, A. et al. (2017) DOI:10.21767/2254-6081-C1-003.

Long Name

Leucine-rich Repeat Containing 24

Alternate Names

LRRC14OS

Entrez Gene IDs

441381 (Human); 378937 (Mouse); 362945 (Rat)

Gene Symbol

LRRC24

UniProt

Product Documents for Recombinant Human LRRC24 His-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human LRRC24 His-tag Protein, CF

For research use only

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