Chinese Hamster Ovary cell line, CHO-derived human LRRC24 protein Cys23-Ser365, with a C-terminal 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
45-55 kDa, under reducing conditions
Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Recombinant Human LRRC24 His-tag, immobilized at 2.5 μg/mL on a 96 well plate, is able to significantly enhance neurite outgrowth.
Recombinant Human LRRC24 His-tag Protein, CF Scientific Data Examples
Recombinant Human LRRC24 His-tag Protein SDS-PAGE.
2 μg/lane of Recombinant Human LRRC24 His-tag (Catalog # 10512-LR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 45-55 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 500 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Leucine-rich repeat-containing protein 24 (LRRC24) is type I transmembrane protein found within the large LRR superfamily. The LRR family is known to have diverse functions, including roles in innate immunity and nervous system development (1). The extracellular domain (ECD) of LRRC24 contains several LRR repeats along with an Ig-like C2-type region. The mature ECD of human LRRC24 shares 86% and 80% amino acid sequence identity with mouse and rat LRRC24, respectively. LRRC24 has been shown to interact with Robo2, which is important for axon guidance and cell migration (2). LRRC24 has been suggested to be a novel negative regulator of the ErbB family and suppress ErbB-driven tumor cell proliferation and motility (3).
Ng, A.C.Y. et al. (2011) PNAS 108:4631.
Söllner, C. and Wright, G.J. (2009) Genome Biol. 10:R99.
Andrews, A. et al. (2017) DOI:10.21767/2254-6081-C1-003.
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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