Recombinant Human LRP-4 Protein, CF

Name: Recombinant Human LRP-4 Protein, CF
Brand: R&D Systems
SKU: 5948-LR

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 5948-LR

Citations (2)

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Key Product Details

Source:	CHO
Accession #:	AAI36669
Applications:	Bioactivity

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Product Specifications for Recombinant Human LRP-4 Protein, CF

Source

Chinese Hamster Ovary cell line, CHO-derived human LRP-4 protein

Human LRP-4 (Ser21-Ser1725) Accession # AAI36669	GGGSGGGSGGGS	6-His
N-terminus		C-terminus

Purity

>80%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Ser21 & Gly212

Predicted Molecular Mass

191 kDa

SDS-PAGE

180-225 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human USAG1 (Catalog # 5370-SD) is coated at 2 μg/mL, Recombinant Human LRP-4 binds with an apparent K_d <1 nM.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5948-LR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 300 μg/mL in PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LRP-4

LDL receptor-related protein 4 (LRP-4), also known as Megf7, is an approximately 220 kDa type I membrane protein in the LDL receptor superfamily. LRP-4 plays an important role in development of the neuromuscular junction (NMJ), bones, teeth, mammary placodes, and hair follicles (1, 2). Mature human LRP-4 consists of a 1705 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 159 aa cytoplasmic domain. The ECD contains 3 EGF-like domains, 8 LDLR-A domains, and 20 LDLR-B repeats that fold into 4 beta-propeller domains (3, 4). Within the ECD, human LRP-4 shares 97% aa sequence identity with mouse and rat LRP-4. The ECD of LRP-4 can be shed by ADAM10 mediated cleavage (5). LRP-4 is expressed on myotubes (6, 7), neurons (8, 9), osteocytes and osteoblasts (10, 11), developing tooth epithelium (12), and male and female germ cells (13). It is localized to NMJ on developing myotubes, and it associates in cis with the muscle-specific kinase MuSK (6, 7). Its binding to neuron-derived Agrin promotes additional LRP-4 association with MuSK, MuSK activation, clustering of AChR, and post-synaptic development of the NMJ (6, 7, 14). LRP-4 also enhances presynaptic differentiation of the NMJ, although this does not require Agrin (14). Neuronal LRP-4 additionally binds ApoE and F-spondin (8, 9). LRP-4 regulates both BMP and Wnt signaling through binding to Wise, Sclerostin, and Dkk1 (10-12). These interactions are important for the control of bone growth and mineralization, tooth morphogenesis, and the development of mammary placodes, hair follicles, and whiskers (10-12, 15).

Item	Value
References	Zong, Y. and R. Jin (2012) Cell. Mol. Life Sci. Nov 22 Epub. Ohazama, A. et al. (2010) Am. J. Med. Genet. A 152A:2974. Nakayama, M. et al. (1998) Genomics 51:27. Tomita, Y. et al. (1998) J. Biochem. 124:784. Dietrich, M.F. et al. (2010) PLoS ONE 5:e9960. Kim, N. et al. (2008) Cell 135:334. Zhang, B. et al. (2008) Neuron 60:285. Lu, Y. et al. (2007) Brain Res. 1177:19. Zisman, S. et al. (2007) J. Cell Biol. 178:1237. Leupin, O. et al. (2011) J. Biol. Chem. 286:19489. Choi, H.Y. et al. (2009) PLoS ONE 4:e7930. Ohazama, A. et al. (2008) PLoS ONE 3:e4092. Yamaguchi, Y.L. et al. (2006) Gene Expr. Patterns 6:607. Yumoto, N. et al. (2012) Nature 489:438. Ahn, Y. et al. (2013) Development 140:583.
Long Name	LDL Receptor-related Protein 4
Entrez Gene IDs	4038 (Human); 228357 (Mouse); 83469 (Rat)
Alternate Names	CLSS, KIAA0816, LRP-4, LRP10, LRP4, MEGF7LRP10, Multiple epidermal growth factor-like domains 7, low density lipoprotein receptor-related protein 4, low-density lipoprotein receptor-related protein 4

Citations for Recombinant Human LRP-4 Protein, CF (2)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human LRP-4 Protein, CF include:

Species: Xenopus
Sample Types: Whole Cells
Applications: Bioassay

MJ Oentaryo et al. (2020), Neuronal MT1-MMP mediates ECM clearance and Lrp4 cleavage for agrin deposition and signaling in presynaptic development J. Cell. Sci., 0(0).
PMID: 32591486
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

K Njah et al. (2019), A Role of Agrin in Maintaining the Stability of Vascular Endothelial Growth Factor Receptor-2 during Tumor Angiogenesis Cell Rep, 28(4):949-965.e7.
PMID: 31340156

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Recombinant Human LRP-4 Protein, CF

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