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Recombinant Human Livin alpha Protein, CF

Catalog # 1161-LV | R&D Systems, Inc. a Bio-Techne Brand
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1161-LV-100

Key Product Details

Source

E. coli

Accession #

Structure / Form

Monomer

Conjugate

Unconjugated

Applications

Inhibition Activity

Product Specifications

Source

E. coli-derived human Livin protein
Met1-Ser298, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.5 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met1

Predicted Molecular Mass

34 kDa

SDS-PAGE

38 kDa, reducing conditions

Activity

Measured by its ability to reverse the inhibition of DEVD-AFC cleavage activity in cell extracts activated by addition of cytochrome c and dATP.
The ED50 for this effect is 0.5-2.0 µM.
Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

1161-LV
Formulation Supplied as a 0.2 μm filtered solution in HEPES, NaCl, DTT and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Livin

Livin alpha is a member of the inhibitor of apoptosis (IAP) protein family and contains a single BIR and RING finger motif. The anti-apoptotic protein appears to inhibit the activation of Caspase-9 in cell extracts activated by cytochrome c and dATP. Livin  alpha is the longer of two splice variants, encoding an additional 18 amino acid residues in the BIR-RING linking region.

References

  1. Kasof, G.M. and B.C. Gomes (2001) J. Biol. Chem. 276(5):3238.
  2. Vucic, D. et al. (2000) Curr. Biol. 10:1359.
  3. Ashhab, Y. et al. (2001) FEBS 495:56.
  4. Lin, J-H. et al. (2000) Biochem. Biophys. Res. Comm. 279:820.

Alternate Names

BIRC7, KIAP, ML-IAP

Entrez Gene IDs

79444 (Human)

Gene Symbol

BIRC7

UniProt

Product Documents for Recombinant Human Livin alpha Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Livin alpha Protein, CF

For research use only

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