Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera Protein, CF

Name: Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 2078-T4

Catalog # 2078-T4 | R&D Systems, Inc. a Bio-Techne Brand

Citations (1)

Product Datasheet / COA / SDS

Setting the standard in quality research reagents

Key Product Details

Source:	NS0
Accession #:	AAH36827.1
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera Protein, CF

Technical Data
Citations (1) / Reviews

Product Specifications for Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera Protein, CF

Source

Mouse myeloma cell line, NS0-derived human LILRB2/CD85d/ILT4 protein

Human LILRB2/CD85d/ILT4 (Gly24-His458) Accession # AAH36827.1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Gly24

Predicted Molecular Mass

73.9 kDa (monomer)

SDS-PAGE

73.9 kDa (monomer)

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human Angiopoietin-like Protein 5/ANGPTL5 (Catalog # 6675-AN) is coated at 4 μg/mL, Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera binds with a typical ED₅₀ of 0.15-0.9 μg/mL. Measured by its ability to support the adhesion of HSB2 human peripheral blood acute lymphoblastic leukemia cells. Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera immobilized at 35 µg/mL, 100 µL/well, will mediate >40% HSB-2 cell adhesion (10⁵ cells/well).

Scientific Data Examples for Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera Protein, CF

	Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera Protein Binding Activity Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera (Catalog # 2078-T4) supports the adhesion of HSB2 human peripheral blood acute lymphoblastic leukemia cells. Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera immobilized at 35 µg/mL, 100 µL/well, will mediate >40% HSB-2 cell adhesion (10⁵cells/well).

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2078-T4

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: LILRB2/CD85d/ILT4

The immunoglobulin-like transcript (ILT) comprise a family of activating and inhibitory type immunoreceptors whose genes are located in the same locus that encodes killer cell Ig-like receptors (KIR) (1-3). ILT4, also known as LIR-2 and LILRB2, is a type I transmembrane protein expressed primarily on monocytes and dendritic cells (DC) (4). Human ILT4 is produced as a 598 amino acid (aa) precursor including a 21 aa signal sequence, a 440 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 116 aa cytoplasmic domain. The ECD contains four Ig-like domains, and the cytoplasmic domain contains three immunoreceptor tyrosine-based inhibitory motifs (ITIM) (5). The ECD of human ILT4 shares 76% aa identity with chimpanzee ILT4 and 74%, 81%, 33%, 52%, 77%, 61%, and 64 % aa identity with human ILT1, 2, 3, 5, 6, 7, and 8, respectively. ILT4 binds to classical MHC I proteins as well as the non-classical HLA-G1 and HLA-F molecules (5-9). It competes with CD8 alpha for MHC I binding but does not compete with KIR2DL1 (7). Ligation of ILT4 induces Tyr phosphorylation within its cytoplasmic ITIMs, a requirement for association with SHP-1 (4, 6). Activation of ILT4 inhibits signaling through Fc gamma RI (4) and Fc epsilon RI (6) and causes DC to become tolerogenic by down-regulation of co‑stimulatory molecules (10, 11). ILT4 mediates tolerogenic DC‑induced CD4⁺ T cell energy in vitro and in vivo (10-12).

Item	Value
References	Suciu-Foca, N. et al. (2005) Int. Immunopharmacol. 5:7. Hofmeister, V. and E.H. Weiss (2003) Semin. Canc. Biol. 13:317. Hunt, J.S. et al. (2005) FASEB J. 19:681. Finger, N.A. et al. (1998) Eur. J. Immunol. 28:3423. Borges, L. et al. (1997) J. Immunol. 159:5192. Colonna, M. et al. (1998) J. Immunol. 160:3096. Shiroishi, M. et al. (2003) Proc. Natl. Acad. Sci. 100:8856. Lepin, E.J.M. et al. (2000) Eur. J. Immunol. 30:3552. Allen, R.L. et al. (2001) J. Immunol. 167:5543. Chang, C.C. et al. (2002) Nat. Immunol. 3:237. Ristich, V. et al. (2005) Eur. J. Immunol. 35:1133. Manavalan, J.S. et al. (2003) Transpl. Immunol. 11:245.
Long Name	Leukocyte Immunoglobulin-like Receptor, Subfamily B (with TM and ITIM Domains), Member 5
Entrez Gene IDs	10288 (Human)
Alternate Names	CD85 antigen-like family member D, CD85d, CD85d antigen, ILT-4, ILT4, ILT4CD85d, Ig-like transcript 4, Immunoglobulin-like transcript 4, LILRB2, LIR-2subfamily A (with TM domain), member 6, LIR2, LIR2CD85D, Leukocyte immunoglobulin-like receptor 2, MIR10, MIR10LILRA6, Monocyte/macrophage immunoglobulin-like receptor 10, leukocyte immunoglobulin-like receptor subfamily B member 2, leukocyte immunoglobulin-like receptor subfamily B member 2 soluble isoform, leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 2

Citations for Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera Protein, CF (1)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera Protein, CF include:

Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance

Lichterfeld M et al. (2007-11-19), A viral CTL escape mutation leading to immunoglobulin-like transcript 4-mediated functional inhibition of myelomonocytic cells. J. Exp. Med., 2007-1204(12):2813-24.
PMID: 18025130

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Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera Protein, CF

Catalog # 2078-T4 | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Product Specifications for Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera Protein, CF

Scientific Data Examples for Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: LILRB2/CD85d/ILT4

Citations for Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera Protein, CF (1)

Product Documents for Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

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