How is the non covalent complex between the prodomain and mature domain of Recombinant Human Latent BMP-9 Protein (Catalog # 9624-BP) produced?

Name: Recombinant Human Latent BMP-9 Protein
Brand: R&D Systems
SKU: 9624-BP
Price: 380 EUR

The prodomain fragment and mature fragment of Recombinant Human Latent BMP-9 Protein (Catalog # 9624-BP) are expressed from the same clone. The two different fragments are purified separately to isolate each fragment. Once each fragment has been isolated, they are mixed together to form the non covalent complex.

Recombinant Human Latent BMP-9 Protein

Catalog # 9624-BP | R&D Systems, Inc. a Bio-Techne Brand

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Key Product Details

Source:	CHO
Accession #:	Q9UK05
Structure / Form:	Non covalent complex between the prodomain and mature domain.
Applications:	Bioactivity

View Full Technical Details for Recombinant Human Latent BMP-9 Protein

Product Specifications for Recombinant Human Latent BMP-9 Protein

Source	Chinese Hamster Ovary cell line, CHO-derived human BMP-9 protein Lys23-Arg319 (pro) & Ser320-Arg429 (mature)
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Lys23 (pro) & Ser320 (mature)
Predicted Molecular Mass	33 kDa (pro) & 12 kDa (mature)
SDS-PAGE	39-44 kDa (pro) & 11 kDa (mature)
Activity	Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED₅₀ for this effect is 1-6 ng/mL.

Scientific Data Examples for Recombinant Human Latent BMP-9 Protein

	Recombinant Human Latent BMP-9 Protein Bioactivity Recombinant Human Latent BMP-9 (Catalog # 9624-BP) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The ED₅₀ for this effect is 1-6 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

9624-BP

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

9624-BP/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BMP-9

Human BMP-9, also known as growth and differentiation factor 2 (GDF2), is a member of the BMP subgroup of the TGF beta superfamily proteins that signal through heterodimeric complexes composed of type I and type II BMP receptors. BMP-9 regulates the development and function of a variety of embryonal and adult tissues (1, 2). The human BMP-9 cDNA encodes a 429 amino acid (aa) precursor that includes a 22 aa signal sequence, a 298 aa propeptide, and a 111 aa mature protein (3). Unlike with other BMP family proteins, the propeptide does not interfere with the biological activity of BMP-9 and remains associated with the mature peptide after proteolytic cleavage (4). Human and mouse BMP-9 share 96% aa sequence identity. Within the mature protein, human BMP-9 shares 64% aa sequence identity with human BMP-10 and less than 50% aa sequence identity with other BMPs. BMP-9 is expressed by nonparenchymal cells in the liver, (5, 6) where it promotes lipid metabolism and inhibits glucose production (7). BMP-9 exerts a prolonged hypoglycemic effect which may be due to an enhancement of insulin release (7). BMP-9 interacts with a high affinity specific heteromeric receptor expressed on liver endothelial cells that has been identified as ALK1 (4, 6). In the embryonal CNS, BMP-9 functions in the development and maintenance of the cholinergic neuronal phenotype (8, 10). BMP-9 also induces the differentiation of mesenchymal stem cells into the chondrogenic lineage (11, 12). At low concentrations, BMP-9 is a proliferative factor for hematopoietic progenitor cells, but at higher concentrations, it enhances TGF-beta 1 production and inhibits hematopoietic progenitor colony formation (13).

Item	Value
References	Chen, D. et al. (2004) Growth Factors 22:233. Miyazono, K. et al. (2005) Cytokine Growth Factor Rev. 16:251. Celeste, A.J. et al. (1994) J. Bone Miner. Res. 9:S136. Brown, M.A. et al. (2005) J. Biol. Chem. 280:25111. Song, J.J. et al. (1995) Endocrinology 136:4293. Miller, A.F. et al. (2000) J. Biol. Chem. 275:17937. Chen, C. et al. (2003) Nat. Biotechnol. 21:294. LopezCoviella,I. et al. (2000) Science 289:313. LopezCoviella,I. et al. (2005) Proc. Natl. Acad. Sci. 102:6984. LopezCoviella,I. et al. (2002) J. Physiol. Paris 96:53. Majumdar, M.K. et al. (2001) J. Cell. Physiol. 189:275. Hills, R.L. et al. (2005) J. Orthoped. Res. 23:611. Ploemacher, R.E. et al. (1999) Leukemia 13:428.
Long Name	Bone Morphogenetic Protein 9
Entrez Gene IDs	2658 (Human); 12165 (Mouse)
Alternate Names	BMP-9, BMP9, BMP9BMP-9Bone morphogenetic protein 9, GDF-2, GDF2, growth differentiation factor 2, growth/differentiation factor 2

Citations for Recombinant Human Latent BMP-9 Protein

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FAQs for Recombinant Human Latent BMP-9 Protein

How is the non covalent complex between the prodomain and mature domain of Recombinant Human Latent BMP-9 Protein (Catalog # 9624-BP) produced?

The prodomain fragment and mature fragment of Recombinant Human Latent BMP-9 Protein (Catalog # 9624-BP) are expressed from the same clone. The two different fragments are purified separately to isolate each fragment. Once each fragment has been isolated, they are mixed together to form the non covalent complex.

Recombinant Human Latent BMP-9 Protein

Catalog # 9624-BP | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Product Specifications for Recombinant Human Latent BMP-9 Protein

Scientific Data Examples for Recombinant Human Latent BMP-9 Protein

Formulation, Preparation and Storage

Carrier Free

9624-BP

Carrier

9624-BP/CF

Carrier Free

Reconstitution Calculator

Background: BMP-9

Citations for Recombinant Human Latent BMP-9 Protein

FAQs for Recombinant Human Latent BMP-9 Protein

Product Documents for Recombinant Human Latent BMP-9 Protein

Product Datasheets

COA

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SDS

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