Skip to main content

Recombinant Human LAIR2 Protein, CF

Catalog # 2665-LR | R&D Systems, Inc. a Bio-Techne Brand
Catalog #
Availability
Size / Price
Qty
Loading...
2665-LR-050

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human LAIR2 protein
Gln22-Pro152, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained: Gln22 predicted & Thr37

Predicted Molecular Mass

14.9 kDa

SDS-PAGE

20 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of HT‑29 human colon adenocarcinoma cells.
When 5 x 104 cells/well are added to rhLAIR-2 coated plates (50 µg/mL with
100 µL/well), approximately 40%-60% will adhere after 10 minutes at 37° C.
Optimal concentration depends on cell type as well as the application or research objectives.

Formulation, Preparation and Storage

2665-LR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Loading...
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LAIR2

LAIR-2 (leukocyte-associated Ig-like receptor-2; CD306) is a secreted, 131 amino acid (aa) protein that contains one Ig-like C2 type domain, making it a member of the Ig superfamily. When compared to LAIR-1, its transmembrane counterpart, it shares 83% aa identity across the signal sequence and extracellular domains; (1-3) although one is secreted and one is membrane-bound, the two LAIR proteins are thought to have arisen from a common gene ancestor and appear to share similar adhesion profiles. This suggests that LAIR-2 may compete with LAIR-1 for ligand binding (3, 4). A 114 aa alternate splice form of LAIR-2 is truncated at the C terminus, but retains the entire Ig domain (1-3). The expression profile of these splice forms, and the presence of orthologs in other species, have not been reported.

References

  1. Meyaard, L. (2003) J. Biol. Regul. Homeost. Agents 17:330.
  2. Meyaard, L. et al. (1999) J. Immunol. 162:5800.
  3. Meyaard, L. et al. (1997)  Immunity 7:283.
  4. Xu, X.G. et al. (2005) Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 21:553.

Long Name

Leukocyte-associated Ig-like Receptor 2

Alternate Names

CD306

Entrez Gene IDs

3904 (Human); 102141809 (Cynomolgus Monkey)

Gene Symbol

LAIR2

UniProt

Product Documents for Recombinant Human LAIR2 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human LAIR2 Protein, CF

For research use only

Citations for Recombinant Human LAIR2 Protein, CF (1)

Showing  1 - 1 of 1 publication Showing All
Filter By:
Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human LAIR2 Protein, CF include:

There are no citations that match your criteria.

Showing  1 - 1 of 1 publication Showing All

Customer Reviews for Recombinant Human LAIR2 Protein, CF (1)

5 out of 5
1 customer rating
5 stars
100%
4 stars
0%
3 stars
0%
2 stars
0%
1 star
0%

Customer Images


Showing  1 - 1 of 1 review Showing All
Filter By:
  • Recombinant Human LAIR2 Protein, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 10/19/2020
    Recombinant Human LAIR2 Protein, CF 2665-LR

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

÷
=
Loading...
Setting the standard in quality research reagents