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Recombinant Human KIR3DL3/CD158z His-tag Protein, CF

Bio-Techne includes R&D Systems | Catalog # 10454-KR

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10454-KR-050

Key Product Details

Source

CHO

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human KIR3DL3/CD158z protein
Gln26-Leu322, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln26, deduced from Asp27 upon deblocking

Predicted Molecular Mass

33 kDa

SDS-PAGE

42-47 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Biotinylated Recombinant Human B7-H7/HHLA2 Fc Chimera is present at 0.5 μg/mL, the concentration of Recombinant Human KIR3D3L/CD158z His-tag that produces 50% of the optimal binding response is found to be approximately 7.5-45 ng/mL.

Scientific Data Images for Recombinant Human KIR3DL3/CD158z His-tag Protein, CF

Recombinant Human KIR3DL3/CD158z His-tag Protein Binding Activity

Recombinant Human KIR3DL3/CD158z His-tag Protein Binding Activity

When Biotinylated Recombinant Human B7-H7/HHLA2 Fc Chimera is present at 0.5 μg/mL, the concentration of Recombinant Human KIR3D3L/CD158z His-tag that produces 50% of the optimal binding response is found to be approximately 7.5-45 ng/mL.
Recombinant Human KIR3DL3/CD158z His-tag Protein SDS-PAGE

Recombinant Human KIR3DL3/CD158z His-tag Protein SDS-PAGE

2 µg/lane of Recombinant Human KIR3DL3/CD158z (Catalog # 10454-KR ) was resolved by SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 42-47 kDa.

Formulation, Preparation and Storage

10454-KR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: KIR3DL3/CD158z

KIR3DL3 (also known as CD158z, KIR3DL7, KIR44, or KIRC1) is a type I transmembrane glycoprotein that belongs to the killer cell Ig-like receptor (KIR) family. KIRs are expressed on CD56dim NK cells and T cell subsets where they regulate effector functions in the innate immune system (1 - 4). KIRs are named for the number of Ig-like domains (2D or 3D) in the extracellular domain (ECD), and whether they have long or short (L, S) cytoplasmic tails. Human KIR3DL3 cDNA encodes a 410 amino acid (aa) polypeptide precursor with a 25 aa signal peptide, a 297 aa extracellular domain (ECD) with 3 Ig-like domains (3D), a 21 aa transmembrane domain, and a 67 aa cytoplasmic domain (long). Within ECD human KIR3DL3 shares 47% and 44% aa sequence identity with mouse and rat KIR3DL3, respectively. KIR3DL3 is ubiquitously present in every individual across diverse populations, however little is known about specific functions (5). The limited knowledge of KIR3DL3 expression does suggest involvement in reproduction, likely during placentation (4). KIR3DL3 likely encodes an NK cell inhibitory receptor (6). Recent studies have shown that KIR3DL3 binding and function require both receptor aggregation and inhibitory signal attenuation (7).

References

  1. Colonna, M. and J. Samaridis (1995) Science 268:405.
  2. Lanier, L. L. (2005) Annu. Rev. Immunol. 23:225.
  3. Uhrberg, M. et al. (1997) Immunity 7:753.
  4. Trundley, A. E. et al. (2006) Immunogenetics 57:904.
  5. Hollenbach, J. A. et al. (2012) Immunogenetics 64:719.
  6. Torkar, M. et al. (1998) Eur. J. Immunol. 28:3959.
  7. Leaton, L. A. et al. (2019) Front. Immunol. 10:24.

Long Name

Killer Cell Immunoglobulin-like Receptor, Three Domain Long Cytoplasmic Tail, 3

Alternate Names

CD158z, KIR3DL7, KIR44, KIRC1

Entrez Gene IDs

115653 (Human)

Gene Symbol

KIR3DL3

UniProt

Additional KIR3DL3/CD158z Products

Product Documents for Recombinant Human KIR3DL3/CD158z His-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human KIR3DL3/CD158z His-tag Protein, CF

For research use only

Reconstitution Calculator

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