Product Specifications for Recombinant Human KIR2DS4/CD158i Fc Chimera Protein, CF
Mouse myeloma cell line, NS0-derived human KIR2DS4/CD158i protein
Human KIR2DS4 (Gln22-His245) Accession # P43632
Human IgG1 (Pro100-Lys330)
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
No results obtained: Gln22 predicted
Predicted Molecular Mass
51.2 kDa (monomer)
70-85 kDa, reducing conditions
Bioassay data are not available.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 100 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
KIR2DS4 (2DS4), previously called NKAT-8 and designated CD158i, is a type I transmembrane glycoprotein that belongs to the killer cell Ig-like receptor (KIR) family (1). KIRs are expressed on CD56dim NK cells and T cell subsets where they differentiate normal from abnormal cells and regulate effector functions in the innate immune system (1-3). KIRs are named for the number of Ig-like domains (2D or 3D) in the extracellular domain (ECD), and whether they have long or short (L, S) cytoplasmic tails. KIR2DS4 cDNA encodes 304 amino acids (aa) including a 21 aa signal sequence, a 224 aa ECD, a 20 aa transmembrane sequence and a 39 aa cytoplasmic domain. KIR receptors have no structural orthologs in nonprimates, although mouse Ly-49 proteins perform similar functions (2). Like other activating KIRs, KIR2DS4 has a short tail and a positively charged amino acid (aa) within the transmembrane domain that interacts with the ITAM-bearing signaling adaptor, DAP12 (2, 3). Expression of activating KIR is balanced with inhibitory KIR to impart specificity to the immune response. KIR2DS4 recognizes six HLA-C allotypes and two HLA-A allotypes (A*1101 and A*1102) but no HLA-B allotypes (4, 5). It has also been suggested that KIR2DS4 recognizes a non-MHC ligand, based on specific binding to melanoma cells that lack MHC Class I (6). A common allele, KIR2DS4*003, is present in up to 89% of Caucasians and ~30% of Asians; it creates a prematurely terminated, soluble, inactive form (7, 8). Lack of functional KIR2DS4 can potentially create unbalanced inhibition of killer cell function (4, 8).
Bottino, C. et al. (1996) Eur. J. Immunol. 26:1816.
Lanier, L. L. (2005) Annu. Rev. Immunol. 23:225.
Purdy, A.K. and K.S. Campbell (2009) Cancer Biol. Ther. 8:13.
Graef, T. et al. (2009) J. Exp. Med. 206:2557.
Katz, G. et al. (2001) J. Immunol. 166:7260.
Katz, G. et al. (2004) J. Immunol. 173:1819.
Maxwell, L.D. et al. (2004) Hum. Immunol. 65:613.
Middleton, D. et al. (2007) Hum. Immunol. 68:128.
Killer Cell Immunoglobulin-like Receptor, Two Domains, Short Cytoplasmic Tail, 4
Entrez Gene IDs
CD158 antigen-like family member I, CD158i, CD158i antigen, KIR antigen 2DS4, KIR2DS4, KKA3KIR1D, MGC120019, MGC125315, MGC125317, MHC class I NK cell receptor, NKAT-8, NKAT8KIR412, Natural killer-associated transcript 8, P58 natural killer cell receptor clones CL-39/CL-17, cl-39, killer cell immunoglobulin-like receptor 2DS4, killer cell immunoglobulin-like receptor, two domains, short cytoplasmic tail4,nkat8, killer inhibitory receptor 4-1-2, natural killer cell inhibitory receptor, p58 NK receptor CL-39/CL-17
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