Recombinant Human KIR2DL1/CD158a Fc Chimera Protein, CF

Name: Recombinant Human KIR2DL1/CD158a Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 1844-KR
Price: 417 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 1844-KR

Citations (5)

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Key Product Details

Source:	NS0
Accession #:	P43626
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

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Product Specifications for Recombinant Human KIR2DL1/CD158a Fc Chimera Protein, CF

Source

Mouse myeloma cell line, NS0-derived human KIR2DL1/CD158a protein

Human KIR2DL1/CD158a (His22-Arg242) Accession # P43626	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE with silver staining

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

His22

Predicted Molecular Mass

51 kDa (monomer)

SDS-PAGE

72-80 kDa, reducing conditions

Activity

Measured by its ability to bind KG‑1A human acute myelogenous leukemia cells in a flow cytometry assay. When 250 ng of Recombinant Human KIR2DL1/CD158a Fc Chimera is added to 1 x 10⁶ KG-1A cells, >55% of the cells will bind to the protein.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1844-KR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: KIR2DL1/CD158a

KIR2DL1 (2DL1, formerly NKAT1, designated CD158a) is a 348 amino acid (aa) type I transmembrane glycoprotein that belongs to the human killer cell Ig-like receptor (KIR) family (1, 2). KIRs are expressed on human CD56dim NK cells and T cell subsets, and regulate effector functions in the innate immune system (1-3). KIRs are named for the number of Ig-like domains (2D or 3D) in the extracellular domain (ECD), and whether they have long or short (L, S) cytoplasmic tails (1-3). Individuals will express varying subsets of inhibiting and activating KIRs with varying polymorphisms (1, 4). Like other inhibiting KIRs, KIR2DL1 has two ITIM domains within its long tail that block activating receptor clustering (2, 5). Within the ECD, KIR2DL1 shares high aa sequence identity (92%) with KIR2DL2. KIR2DL1 targets Lys80-containing HLA-C2 allotypes while KIR2DL2 rather recognizes Ans80 containing HLA-C1 (1, 6-8). Three KIRs proteins together (2DL1-3) recognize and inhibit NK cytotoxicity against cells expressing any HLA-C allotype, allowing self-recognition, but also conferring susceptibility to leukemia (1-3). In primary human NK cell clones, KIR2DL1 regulates the level of MHC I proteins expression required for NK cell inhibition (9).

Item	Value
References	Purdy, A.K. and Campbell, K.S. (2009) Cancer Biol. Ther. 8:13. Lanier, L. L. (2005) Annu. Rev. Immunol. 23:225. Kulkarni, S. et al. (2008) Sem. Immunol. 20:343. Middleton, D. and F. Gonzelez (2009) Immunology 129:8. Abeyweera, T.P. et al. (2011) J. Cell Biol. 192:675. Moesta, A.K. et al. (2008) J. Immunol. 180:3969. Boyington, J.C. et al. (2000) Nature 405:537. Snyder, G.A. et al. (1999) Proc. Natl. Acad. Sci. USA 96:3864. Almeida, C.R. et al. (2011) PLoS ONE. 6:e24927.
Long Name	Killer Cell Immunoglobulin-like Receptor, Two Domain Long Cytoplasmic Tail, 1
Entrez Gene IDs	3802 (Human)
Alternate Names	CD158 antigen-like family member A, CD158Ankat1, CD158a, CD158a antigen, KIR221, KIR2DL1, MHC class I NK cell receptor, NKAT, NKAT-1, NKAT1KIR-K64, Natural killer-associated transcript 1, cl-42, killer Ig receptor, killer cell immunoglobulin-like receptor 2DL1, killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 1, killer inhibitory receptor 2-2-1, p58 NK cell inhibitory receptor NKR-K6,47.11, p58 NK receptor CL-42/47.11, p58 killer cell inhibitory receptor KIR-K64, p58 natural killer cell receptor clones CL-42/47.11, p58.1, p58.1 MHC class-I-specific NK receptor

Citations for Recombinant Human KIR2DL1/CD158a Fc Chimera Protein, CF (5)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human KIR2DL1/CD158a Fc Chimera Protein, CF include:

Species: Human
Sample Types: Recombinant Proteins
Applications: Bioassay

P Fittje et al. (2022), HIV-1 Nef-mediated downregulation of CD155 results in viral restriction by KIR2DL5+ NK cells Oncogene, 18(6):e1010572.
PMID: 35749424
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

N Wauquier et al. (2019), HLA-C-restricted viral epitopes are associated with an escape mechanism from KIR2DL2+ NK cells in Lassa virus infection EBioMedicine, 0(0).
PMID: 30711514
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

MJW Sim et al. (2019), Human NK cell receptor KIR2DS4 detects a conserved bacterial epitope presented by HLA-C Proc. Natl. Acad. Sci. U.S.A., 0(0).
PMID: 31138701
Species: N/A
Sample Types: Protein
Applications: Bioassay

A Chapel et al. (2017), Peptide-specific engagement of the activating NK cell receptor KIR2DS1 Sci Rep, 7(1):2414.
PMID: 28546555
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Cifaldi L et al. (2015), ERAP1 regulates natural killer cell function by controlling the engagement of inhibitory receptors. Cancer Res, 75(5):824-34.
PMID: 25592150

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Showing 1 - 5 of 5 citations Showing All

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Recombinant Human KIR2DL1/CD158a Fc Chimera Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

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