Recombinant Human KGF/FGF-7 Protein, CF New

Name: Recombinant Human KGF/FGF-7 Protein, CF
Brand: R&D Systems
SKU: 11340-KG
Price: 253 USD

Catalog # 11340-KG | R&D Systems, Inc. a Bio-Techne Brand

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Key Product Details

Source:	HEK293
Accession #:	P21781.1
Applications:	Bioactivity

View Full Technical Details for Recombinant Human KGF/FGF-7 Protein, CF

Product Specifications for Recombinant Human KGF/FGF-7 Protein, CF

Source	Human embryonic kidney cell, HEK293-derived human KGF/FGF-7 protein Cys32-Thr194
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Cys32
Predicted Molecular Mass	19 kDa
SDS-PAGE	22-28 kDa, under reducing conditions.
Activity	Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED₅₀ for this effect is 25.0-250 ng/mL.

Scientific Data Examples for Recombinant Human KGF/FGF-7 Protein, CF

	Recombinant Human KGF/FGF‑7 Protein Bioactivity. Recombinant Human KGF/FGF-7 Protein (Catalog # 11340-KG) stimulates cell proliferation of MCF-7 human breast cancer cells, The ED₅₀ for this effect is 25.0-250 ng/mL.
	Recombinant Human KGF/FGF‑7 Protein SDS-PAGE. 2 μg/lane of Recombinant Human KGF/FGF‑7 Protein (Catalog # 11340-KG) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 22-28 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11340-KG

Formulation	Lyophilized from a 0.2 μm filtered solution in MOPS, Na₂SO₄ and EDTA with Trehalose.
Reconstitution	Reconstitute 10 μg size at 100 μg/mL and all the other sizes at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: KGF/FGF-7

KGF (keratinocyte growth factor), also known as FGF-7 (fibroblast growth factor-7), is one of 22 known members of the mouse FGF family of secreted proteins that plays a key role in development, morphogenesis, angiogenesis, wound healing, and tumorigenesis (1-4,13). KGF expression is restricted to cells of mesenchymal origin. When secreted, it acts as a paracrine growth factor for nearby epithelial cells (1). KGF speeds wound healing by being dramatically upregulated in response to damage to skin or internal structures that results in high local concentrations of inflammatory mediators such as IL-1 and TNF-alpha. (2, 5). KGF promotes cell migration and invasion, and mediates melanocyte transfer to keratinocytes upon UVB radiation (6, 7). It has been used ectopically to avoid chemotherapy-induced oral mucositis in patients with hematological malignancies (1). Deletion of KGF affects kidney development, producing abnormally small ureteric buds and fewer nephrons (8). It also impedes hair follicle differentiation (9). The 194 amino acid (aa) KGF precursor contains a 31 aa signal sequence and, like all other FGFs, an ~120 aa beta -trefoil scaffold that includes receptor- and heparin-binding sites. KGF signals only through the IIIb splice form of the tyrosine kinase receptor, FGF R2 (FGF R2-IIIb/KGF R) (10). Receptor dimerization requires an octameric or larger heparin or heparin sulfate proteoglycan (11). FGF-10, also called KGF2, shares 51% aa identity and similar function to KGF, but shows more limited expression than KGF and uses an additional receptor, FGF R2-IIIc (12). Following receptor engagement, KGF is typically degraded, while FGF-10 is recycled (12). Mature human KGF, which is active across species, shares 98% aa sequence identity with bovine, equine, ovine and canine, 96% with mouse and porcine, and 92% with rat KGF, respectively.

Item	Value
References	Finch, P.W. and J.S. Rubin (2006) J. Natl. Cancer Inst. 98:812. Werner, S. et al. (2007) J. Invest. Dermatol. 127:998. Werner, S. (1998) Cytokine Growth Factor Rev. 9:153. Mason, I.J. et al. (1994) Mech. Dev. 45:15. Geer, D.J. et al. (2005) Am. J. Pathol. 167:1575. Niu, J. et al. (2007) J. Biol. Chem. 282:6001. Cardinali, G. et al. (2005) J. Invest. Dermatol. 125:1190. Qiao, J. et al. (1999) Development 126:547. Guo, L. et al. (1996) Genes Dev. 10:165. de Georgi, V. et al. (2007) Dermatol. Clin. 25:477. Hsu, Y-R. et al. (1999) Biochemistry 38:2523. Belleudi, F. et al. (2007) Traffic 8:1854. Vadija R. et al. (2016) J Chem Biol. 9:69.
Long Name	Keratinocyte Growth Factor
Entrez Gene IDs	2252 (Human); 403915 (Canine)
Alternate Names	FGF-7, FGF7, HBGF-7, HBGF7, Heparin-binding growth factor 7, KGF, KGFfibroblast growth factor 7 (keratinocyte growth factor), fibroblast growth factor 7HBGF-7, keratinocyte growth factor

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Recombinant Human KGF/FGF-7 Protein, CF New

Catalog # 11340-KG | R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Human KGF/FGF-7 Protein, CF

Scientific Data Examples for Recombinant Human KGF/FGF-7 Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: KGF/FGF-7

Citations for Recombinant Human KGF/FGF-7 Protein, CF

Product Documents for Recombinant Human KGF/FGF-7 Protein, CF

Product Datasheets

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