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Recombinant Human KGF/FGF-7 GMP Protein, CFGMP


Order Information
Key Product Details
Source: | E. coli |
Accession #: | P21781 |
Applications: | Bioactivity |
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Product Specifications
Source |
E. coli-derived human KGF/FGF-7 protein Cys32-Thr194, with an N-terminal Met Manufactured and tested under cGMP guidelines. |
Purity | >97%, by SDS-PAGE with silver staining, under reducing conditions. |
Endotoxin level | <0.10 EU per 1 μg of the protein by the LAL method. |
N-terminal sequence Analysis |
Met-(Cys)32-Asn-Asp-Met-Thr-Pro-Glu-Gln-Met (Cys)32-Asn-Asp-Met-Thr-Pro-Glu-Gln-Met-Ala |
Predicted Molecular Mass | 19 kDa |
SDS-PAGE | 20 kDa, reducing conditions |
Activity |
Measured in a cell proliferation assay using 4MBr‑5 rhesus monkey epithelial cells. Rubin, J.S. et al. (1989) Proc. Natl. Acad. Sci. USA 86:802. The ED50 for this effect is 6-60 ng/mL. The specific activity of recombinant human KGF/FGF-7 is >8.16 x 105 units/mg, which is calibrated against the human KGF/FGF-7 WHO Standard (NIBSC code: 03/150). |
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Recombinant Human KGF/FGF-7 GMP Protein Bioactivity GMP-grade Recombinant Human KGF/FGF-7 (Catalog # 251-GMP) stimulates pro-liferation of the 4MBr‑5 rhesus monkey epithelial cell line. The ED50for this effect is 6-60 ng/mL. |
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Recombinant Human KGF/FGF-7 GMP Protein SDS-PAGE 1 μg/lane of GMP-grade Recombinant Human KGF/FGF-7 (Catalog # 251-GMP) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 20 kDa. |
Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution | Reconstitute at 100 μg/mL in PBS. |
Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: KGF/FGF-7
KGF (keratinocyte growth factor), also known as FGF-7 (fibroblast growth factor-7), is one of 22 known members of the mouse FGF family of secreted proteins that plays a key role in development, morphogenesis, angiogenesis, wound healing, and tumorigenesis (1-4). KGF expression is restricted to cells of mesenchymal origin. When secreted, it acts as a paracrine growth factor for nearby epithelial cells (1). KGF speeds wound healing by being dramatically upregulated in response to damage to skin or internal structures that results in high local concentrations of inflammatory mediators such as IL-1 and TNF-alpha. (2, 5). KGF promotes cell migration and invasion, and mediates melanocyte transfer to keratinocytes upon UVB radiation (6, 7). It has been used ectopically to avoid chemotherapy-induced oral mucositis in patients with hematological malignancies (1). Deletion of KGF affects kidney development, producing abnormally small ureteric buds and fewer nephrons (8). It also impedes hair follicle differentiation (9). The 194 amino acid (aa) KGF precursor contains a 31 aa signal sequence and, like all other FGFs, an ~120 aa beta -trefoil scaffold that includes receptor- and heparin-binding sites. KGF signals only through the IIIb splice form of the tyrosine kinase receptor, FGF R2 (FGF R2-IIIb/KGF R) (10). Receptor dimerization requires an octameric or larger heparin or heparin sulfate proteoglycan (11). FGF-10, also called KGF2, shares 51% aa identity and similar function to KGF, but shows more limited expression than KGF and uses an additional receptor, FGF R2-IIIc (12). Following receptor engagement, KGF is typically degraded, while FGF-10 is recycled (12). Mature human KGF, which is active across species, shares 98% aa sequence identity with bovine, equine, ovine and canine, 96% with mouse and porcine, and 92% with rat KGF, respectively.
Item | Value |
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References |
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Long Name | Keratinocyte Growth Factor |
Entrez Gene IDs | 2252 (Human); 403915 (Canine) |
Alternate Names | FGF-7, FGF7, HBGF-7, HBGF7, Heparin-binding growth factor 7, KGF, KGFfibroblast growth factor 7 (keratinocyte growth factor), fibroblast growth factor 7HBGF-7, keratinocyte growth factor |
Citations
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