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Recombinant Human Keratocan His-tag Protein, CF

Catalog # 10838-KE | R&D Systems, Inc. a Bio-Techne Brand
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10838-KE-050

Key Product Details

Source

CHO

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Keratocan protein
Arg21-Ile352, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg21

Predicted Molecular Mass

40 kDa

SDS-PAGE

44-62 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Keratocan His-tag is immobilized at 1.00 μg/mL (100 µL/well), Recombinant Human BLMH/Bleomycin Hydrolase  (Catalog # 6200-CY) binds with an ED50 of 1.20-12.0 μg/mL.

Scientific Data Images for Recombinant Human Keratocan His-tag Protein, CF

Recombinant Human Keratocan His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human Keratocan His-tag (Catalog # 10838-KE) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 44-62 kDa.

Formulation, Preparation and Storage

10838-KE
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 1 mg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Keratocan

Keratocan is a keratan sulfate-containing proteoglycan that is highly expressed in human cornea (1, 2). It is an extracellular matrix protein that belongs to the class II small leucine-rich proteoglycan family (1). This class of protein contains 10-12 leucine-rich repeat motifs flanked by conserved cysteine residues in N-and C-terminal domains of the core proteins (1, 3). Human keratocan is expressed as a 352 amino acid (aa) protein that includes a 20 aa signal peptide and the 332 aa keratocan protein containing ten LRR motifs (2). Human keratocan shares 87% and 88% aa identity with mouse and rat keratocan, respectively. Keratocan is expressed in the cornea and tendon where it is likely to play a role in collagen fibrillogenesis and in maintaining corneal clarity (4, 5). Keratocan is also expressed in the bone and can modulate osteogenic differentiation (6).

References

  1. Kao, W. et al. (2003) Ocul Surf. 1:5.
  2. Pellegata, N.S. et al. (2000) Nat. Genet. 25:91.
  3. Ameye, L. et al. (2002) Glycobiology 12:107R.
  4. Liu, C. et al. (2003) J. Biol. Chem. 278:21672.
  5. Rees, S.G. et al. (2009) Osteoarthritis Cartilage 17:276.
  6. Igwe, J.C. et al. (2011) Connect. Tissue Res. 52:401.

Long Name

Keratocan

Alternate Names

KERA, SLRR2B

Entrez Gene IDs

11081 (Human); 16545 (Mouse)

Gene Symbol

KERA

UniProt

Product Documents for Recombinant Human Keratocan His-tag Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Keratocan His-tag Protein, CF

For research use only

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