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Recombinant Human Kallikrein 5 Protein, CF

Bio-Techne includes R&D Systems | Catalog # 1108-SE

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1108-SE-010

Key Product Details

Source

NS0

Accession #

Structure / Form

Mature form

Conjugate

Unconjugated

Applications

Enzyme Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Kallikrein 5 protein
Ile67-Ser293, with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ile67

Predicted Molecular Mass

27 kDa

SDS-PAGE

37-42 kDa, reducing conditions

Activity

Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC (Catalog # ES011).
The specific activity is >200 pmol/min/µg, as measured under the described conditions. 

Scientific Data Images for Recombinant Human Kallikrein 5 Protein, CF

Recombinant Human Kallikrein 5 Protein Enzyme Activity

Recombinant Human Kallikrein 5 Protein Enzyme Activity

Recombinant Human Kallikrein 5 (Catalog # 1108-SE) is measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC (Catalog # ES011).

Formulation, Preparation and Storage

1108-SE
Formulation Supplied as a 0.2 μm filtered solution in MES, NaCl and Glycerol.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Kallikrein 5

Human tissue Kallikrein 5 (hK5, also known as stratum corneum tryptic enzyme) is a serine protease of the human tissue Kallikrein gene family (2). The deduced amino acid sequence of human KLK5 consists of a signal peptide (Met1 to Gly22), a pro region (Val23 to Arg66) and a mature/active enzyme (Ile67 to Ser293). KLK5 is expressed mainly in skin, breast, brain and testis and to a lesser extent in many other tissues (3). KLK5 overexpression is an indicator of poor prognosis in ovarian cancer (4). hK5 is a candidate physiological activator of hK7 in the stratum corneum (5). Indeed, rhK5 can be used to activate rhK7 (R&D Systems, Catalog # 2624‑SE) by incubating rhK5 with recombinant human (rh) K7 (both at 0.1 mg/mL) in 25 mM MES, 0.15 M NaCl, pH 5.5, at 37 °C overnight.

rhK5 enzymatic activity can be inhibited by rhSerpinA1, A5, F2, and G1 (R&D Systems, Catalog # 1268‑PI, 1266‑PI, 1470-PI, 2488‑PI).

References

  1. Brattsand, M. and T. Egelrud (1999) J. Biol. Chem. 274:30033.
  2. Yousef, G.M. et al. (1999) Anticancer Res. 19:2843.
  3. Yousef, G.M. and E.P. Diamandis (2001) Endocrine Rev. 22:184.
  4. Yousef, G.M. and E.P. Diamandis (1999) J. Biol. Chem. 274:37511.
  5. Kim, H. et al. (2001) Br. J. Cancer 84:643.

Alternate Names

KLK-L2, KLK5, SCTE

Entrez Gene IDs

25818 (Human); 68668 (Mouse); 690603 (Rat)

Gene Symbol

KLK5

UniProt

Additional Kallikrein 5 Products

Product Documents for Recombinant Human Kallikrein 5 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Kallikrein 5 Protein, CF

For research use only

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