Recombinant Human Kallikrein 4/Prostase Protein, CF

Name: Recombinant Human Kallikrein 4/Prostase Protein, CF
Brand: R&D Systems
SKU: 1719-SE
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 1719-SE

Citations (5)

(1)

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Key Product Details

Source:	NS0
Accession #:	NP_004908
Structure / Form:	Pro form
Applications:	Enzyme Activity

View Full Technical Details for Recombinant Human Kallikrein 4/Prostase Protein, CF

Product Specifications for Recombinant Human Kallikrein 4/Prostase Protein, CF

Source	Mouse myeloma cell line, NS0-derived human Kallikrein 4/Prostase/EMSP1 protein Ser27-Ser254, with a C-terminal 10-His tag
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ser27 & Ser29
Predicted Molecular Mass	26 kDa
SDS-PAGE	33 kDa, reducing conditions
Activity	Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC (Catalog # ES011). The specific activity is >250 pmol/min/µg, as measured under the described conditions.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1719-SE

Formulation	Lyophilized from a 0.2 μm filtered solution in Tris and NaCl.
Reconstitution	Reconstitute at 200 μg/mL in sterile 50 mM Tris, 10 mM CaCl₂ and 150 mM NaCl, pH 7.5.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: Kallikrein 4/Prostase/EMSP1

Kallikrein 4 (KLK4), also known as prostase or enamel matrix serine protease 1 (EMSP1), is a serine protease of the human tissue kallikrein gene family (1). Among normal tissues, human KLK4 is specifically expressed in the prostate (2). It is over-expressed in prostate cancer and this expression is regulated by hormones including androgens, esterogen and progesterone (3). rhKLK4 readily activates pro-KLK3/PSA and pro-urokinase type plasminogen activator (uPA), indicating it may initiate events involving PSA and uPA in either normal or abnormal processes (4). KLK4 may have additional roles such as functioning as one of the two major enamel proteases identified that process enamel matrix proteins (5). In addition to being a secreted enzyme, it is also a nuclear protein (3, 6). The deduced amino acid sequence of human KLK4 consists of a signal peptide, a short pro region and a mature/active enzyme. rhKLK4 can be activated in vitro by thermolysin, a zinc protease. The peptidase activity can be inhibited by AEBSF (R&D Systems, Catalog # EI001), a general serine protease inhibitor.

Item	Value
References	Yousef, G.M. and E.P. Diamandis (2001) Endocrine Rev. 22:184. Nelson, P.S. et al. (1999) Proc. Natl. Acad. Sci. USA 96:3114. Xi, Z. et al. (2004) Cancer Res. 64:2365. Takayama, T.K. et al. (2001) Biochemistry 40:15341. Simmer, J.P. and J.C. Hu (2002) Connect Tissue Res. 43:441. Ryu, O.H. et al. (2002) Eur. J. Oral. Sci. 110:358.
Entrez Gene IDs	9622 (Human)
Alternate Names	AI2A1, ARM1, EC 3.4.21, EC 3.4.21.-, EMSP, EMSP1, EMSP1MGC116827, Enamel matrix serine proteinase 1, KLK-L1MGC116828, KLK4, Kallikrein 4, Kallikrein-like protein 1, PRSS17enamel matrix serine protease 1, PSTSandrogen-regulated message 1, Prostase, Serine protease 17, kallikrein 4 (prostase, enamel matrix, prostate), kallikrein-4, kallikrein-related peptidase 4

Citations for Recombinant Human Kallikrein 4/Prostase Protein, CF (5)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Kallikrein 4/Prostase Protein, CF include:

Species: Human
Sample Types: Protein
Applications: Bioassay

R Williams et al. (2020), Pathogenesis of Molar Hypomineralisation: Hypomineralised 6-Year Molars Contain Traces of Fetal Serum Albumin Front Physiol, 11(0):619.
PMID: 32595522
Species: Human
Sample Types: Antibody
Applications: Surface Plasmon Resonance

Sexton DJ et al. (2009), Specific inhibition of tissue kallikrein 1 with a human monoclonal antibody reveals a potential role in airway diseases. Biochem. J., 422(2):383-92.
PMID: 19527222
Species: Human
Sample Types: Protein
Applications: Enzyme Assay

Mukai S et al. (2008), Activation of hepatocyte growth factor activator zymogen (pro-HGFA) by human kallikrein 1-related peptidases. FEBS J., 275(5):1003-17.
PMID: 18221492
Species: N/A
Sample Types: Peptide
Applications: Enzyme Assay

Yoon H et al. (2007), Activation profiles and regulatory cascades of the human kallikrein-related peptidases. J. Biol. Chem., 282(44):31852-64.
PMID: 17823117
Species: Human
Sample Types: Enzyme Substrate

Kantyka T et al. (0), Inhibition of kallikrein-related peptidases by the serine protease inhibitor of Kazal-type 6. Peptide32(6):1187-92.
PMID: 21439340

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Customer Reviews for Recombinant Human Kallikrein 4/Prostase Protein, CF (1)

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Recombinant Human Kallikrein 4/Prostase Protein, CF

Name: Anonymous

Application: Enzymatic activity in vitro

Verified Customer | Posted 08/10/2017

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Product Documents for Recombinant Human Kallikrein 4/Prostase Protein, CF

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Recombinant Human Kallikrein 4/Prostase Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Human Kallikrein 4/Prostase Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: Kallikrein 4/Prostase/EMSP1

Citations for Recombinant Human Kallikrein 4/Prostase Protein, CF (5)

Customer Reviews for Recombinant Human Kallikrein 4/Prostase Protein, CF (1)

Recombinant Human Kallikrein 4/Prostase Protein, CF

Product Documents for Recombinant Human Kallikrein 4/Prostase Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

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