Recombinant Human Kallikrein 14 Protein, CF

Name: Recombinant Human Kallikrein 14 Protein, CF
Brand: R&D Systems
SKU: 2626-SE
Rating: 5 (1 reviews)

Catalog # 2626-SE | R&D Systems, Inc. a Bio-Techne Brand

(1)

Citations (6)

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Key Product Details

Source:	NS0
Accession #:	AAD50773
Structure / Form:	Pro form
Applications:	Enzyme Activity

View Full Technical Details for Recombinant Human Kallikrein 14 Protein, CF

Product Specifications for Recombinant Human Kallikrein 14 Protein, CF

Source	Mouse myeloma cell line, NS0-derived human Kallikrein 14 protein Gln19-Met248, with a C-terminal 10-His tag
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin level	<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	No results obtained: Gln19 predicted
Predicted Molecular Mass	26 kDa
SDS-PAGE	28 kDa, reducing conditions
Activity	Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC (Catalog # ES011). The specific activity is >3,000 pmol/min/µg, as measured under the described conditions.

Scientific Data Examples for Recombinant Human Kallikrein 14 Protein, CF

	Recombinant Human Kallikrein 14 Protein Enzyme Activity Recombinant Human Kallikrein 14 (Catalog # 2626-SE) is measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC (Catalog # ES011).

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2626-SE

Formulation	Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

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Background: Kallikrein 14

Human tissue kallikreins refer to a group of secreted serine proteases that are encoded by homologous genes clustering on chromosome 19q13.3-4. As a member of this family, human tissue Kallikrein 14 (hKLK14) is present in many tissues, with high levels in breast, skin, prostate, and brain. The 251 amino acid hKLK14 precursor consists of a signal peptide (residues 1 to 18), a pro peptide (residues 19 to 24) and an active protein (residues 25 to 251) (1). Its enzymatic activity has been shown to be mainly trypsin-like (2). However, its physiological substrates and functions are still unclear. Several studies have suggested that hKLK14 may have clinical utility as a biomarker for cancer of the breast, ovary, and prostate (3, 4). In addition, it may be the initiator of a kallikrein proteolytic cascade responsible for the degradation of the adhesion structures in the stratum corneum (2). The purified, secreted rhKLK14 corresponds to the pro form (residues 19 to 248) with a replacement of the last three residues with a His tag at the C-terminus. When activated by thermolysin, it displays enzymatic activity towards a fluorogenic peptide described above. This activity can be inhibited by recombinant human Serpin A4, E1, and F2 (Catalog # 1669-PI, 1786-PI, and 1470-PI, respectively).

Item	Value
References	Yousef, G. M. et al. (2001) Cancer Res. 61:3425. Brattsand, M. et al. (2004) J. Invest. Dermatol. 124:198. Borgono, C. et al. (2003) Cancer Res. 63:9032. Yousef, G. M. et al. (2003) Prostate. 56:287.
Entrez Gene IDs	43847 (Human)
Alternate Names	EC 3.4.21, EC 3.4.21.-, KLK-L6kallikrein-14, KLK14, KLKL6, Kallikrein 14, Kallikrein-like protein 6, hK14, kallikrein-related peptidase 14

Citations for Recombinant Human Kallikrein 14 Protein, CF (6)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Kallikrein 14 Protein, CF include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Fischer J et al. (2013-01-10), Cross-linking of SPINK6 by transglutaminases protects from epidermal proteases. J Invest Dermatol, 2013-0133(5):1170-7.
PMID: 23303447
Species: Human
Sample Types: Substrate
Applications: Enzyme Assay

Fortugno P et al. (2011-06-23), Proteolytic activation cascade of the Netherton syndrome-defective protein, LEKTI, in the epidermis: implications for skin homeostasis. J Invest Dermatol, 2011-0131(11):2223-32.
PMID: 21697885
Sample Types: Protein
Applications: Bioassay

Kantyka T et al. (2011-03-23), Inhibition of kallikrein-related peptidases by the serine protease inhibitor of Kazal-type 6. Peptides, 2011-032(6):1187-92.
PMID: 21439340
Species: Human
Sample Types: Peptide
Applications: Enzyme Assay

Meyer-Hoffert U et al. (2010-07-28), Isolation of SPINK6 in human skin: selective inhibitor of kallikrein-related peptidases. J. Biol. Chem., 2010-0285(42):32174-81.
PMID: 20667819
Species: Human
Sample Types: Antibody
Applications: Surface Plasmon Resonance

Sexton DJ et al. (2009-08-13), Specific inhibition of tissue kallikrein 1 with a human monoclonal antibody reveals a potential role in airway diseases. Biochem. J., 2009-0422(2):383-92.
PMID: 19527222
Sample Types: Peptide
Applications: Enzyme Assay

Yoon H et al. (2007-09-06), Activation profiles and regulatory cascades of the human kallikrein-related peptidases. J. Biol. Chem., 2007-0282(44):31852-64.
PMID: 17823117

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Recombinant Human Kallikrein 14 Protein, CF

Name: Anonymous

Application: Enzymatic activity in vitro

Verified Customer | Posted 04/11/2018

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Product Documents for Recombinant Human Kallikrein 14 Protein, CF

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Recombinant Human Kallikrein 14 Protein, CF

Catalog # 2626-SE | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Product Specifications for Recombinant Human Kallikrein 14 Protein, CF

Scientific Data Examples for Recombinant Human Kallikrein 14 Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: Kallikrein 14

Citations for Recombinant Human Kallikrein 14 Protein, CF (6)

Customer Reviews for Recombinant Human Kallikrein 14 Protein, CF (1)

Recombinant Human Kallikrein 14 Protein, CF

Product Documents for Recombinant Human Kallikrein 14 Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

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