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Recombinant Human Kallikrein 11 Protein, CF

Bio-Techne includes R&D Systems | Catalog # 1595-SE

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1595-SE-010

Key Product Details

Source

NS0

Accession #

Structure / Form

Pro form

Conjugate

Unconjugated

Applications

Enzyme Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Kallikrein 11 protein
Glu19-Asn250, with an N-terminal signal peptide and a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu19

Predicted Molecular Mass

27 kDa

SDS-PAGE

40 kDa, reducing conditions

Activity

Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-Lys-SBzl), in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB). Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468.
The specific activity is >200 pmol/min/µg, as measured under the described conditions.

Formulation, Preparation and Storage

1595-SE
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and CaCl2.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Kallikrein 11

As a member of human tissue kallikrein family, Kallikrein 11, also known as hippostasin, trypsin-like serine protease and PRSS20, is encoded by the KLK11 gene (1). Two alternatively spliced forms exist, resulting in 250 (isoform 1) and 282 (isoform 2) amino acid sequences,respectively (2-5). Isoform 1 consists of a signal peptide (residues 1-18), a short pro peptide (residues 19-21) and the mature chain (residues 22-250). Isoform 2 is identical to isoform 1, except that a 32 amino acid segment is inserted in isoform 2 before residue 1 in isoform 1. Isoform 1 is predominantly expressed in brain whereas isoform 2 is preferentially expressed in prostate. KLK11 is a novel marker for ovarian and prostate cancer carcinomas (6-8). Recombinant human KLK11, after being activated by thermolysin, is active against a thioester substrate. This activity can be inhibited by AEBSF (Catalog # http://www.rndsystems.com/product_results.aspx?k=EI001">EI001), dichloroisocoumarin, and aprotinin. Recombinant human KLK11 produced by R&D Systems corresponds to isoform 1.

References

  1. Yousef, G.M. and E.P. Diamandis (2001) Endocrine Rev. 22:184.
  2. Yoshida, S. et al. (1998) Biochim. Biophys. Acta 1399:225. 
  3. Yousef, G.M. et al. (2000) Genomics 63:88.
  4. Mitsui, S. et al. (2000) Biochem. Biophys. Res. Commun. 272:205.
  5. Gan, L. et al. (2000) Gene 257:119.
  6. Diamandis, E.P. et al. (2002) Cancer Res. 62:295.
  7. Nakamura, T. et al. (2003) Urology 61:1042.
  8. Borgono, C.A. et al. (2003) Int. J. Cancer 106:605.

Alternate Names

KLK11

Entrez Gene IDs

11012 (Human)

Gene Symbol

KLK11

UniProt

Additional Kallikrein 11 Products

Product Documents for Recombinant Human Kallikrein 11 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Kallikrein 11 Protein, CF

For research use only

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