Skip to main content

Novus Biologicals products are now on

Better as one

Recombinant Human ITIH1 His-tag Protein, CF

Bio-Techne includes R&D Systems | Catalog # 10268-IT

Catalog #
Size / Price

Key Product Details



Accession #





Product Specifications


Chinese Hamster Ovary cell line, CHO-derived human ITIH1 protein
Ser30-Asp672, with a C-terminal 6-His tag


>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis


Predicted Molecular Mass

73 kDa


79-82 kDa, under reducing conditions


Measured by its binding ability in a functional ELISA.
When Recombinant Human ITIH1 is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Human TSG‑6 (Catalog # 2104-TS) that produces 50% of the optimal binding response is 1-6 μg/mL

Scientific Data Images for Recombinant Human ITIH1 His-tag Protein, CF

Recombinant Human ITIH1 His-tag Protein Binding Activity

Recombinant Human ITIH1 His-tag Protein Binding Activity

When Recombinant Human ITIH1 (Catalog # 10268-IT) is coated at 2 µg/mL, 100 µL/well, Recombinant Human TSG-6 (Catalog # 2104-TS) binds with an ED50 of 1-6 µg/mL.
Recombinant Human ITIH1 His-tag Protein SDS-PAGE

Recombinant Human ITIH1 His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human ITIH1 His-tag was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing a band at 80 kDa under reducing conditions.

Formulation, Preparation and Storage

Formulation Lyophilized from a 0.2 μm filtered solution in Tris and NaCl.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ITIH1

Inter-alpha-trypsin inhibitor heavy chain 1 (ITIH1) is a heavy chain (HC) member of the ITIH family that is synthesized in the liver and circulates in the plasma (1). ITIH1 contains a signal sequence, propeptide, and a conserved von-Willebrand type A domain as in other HCs in the family. ITIH1 also includes a c-terminal extension multicopper oxidase domain, present in ITIH3 as well, that is trimmed to reveal a c-terminal aspartic acid residue that enables crosslinking of the HC to chondroitin sulfate (CS) (1,2). ITIH1 represents the HC1 component of Inter-alpha -inhibitor.  Inter-alpha -inhibitor is a protease inhibitor composed of three subunits (HC1, HC2, and bikunin), which are linked together via a CS moiety (1,3). ITIH1 can alternatively be associated with hyaluronan (HA) to form the Serum-derived hyaluronan associated protein (SHAP)-hyaluronan (HA) complex known to assist in stabilizing HA-rich extracellular matrices in the context of inflammatory processes and ovulation (3,4).  Tumor necrosis factor-stimulated gene-6 (TSG-6) has high affinity for heavy chains and plays an important role in the transfer of HC to HA matrix by forming a TSG-6:HC complex intermediate required for the transfer (4,5). Inter-alpha -inhibitor heavy chains potentiate CD-44-mediated leukocytes adhesion to hyaluronan substratum and recruit immune cells to sites of inflammation (6).  The SHAP-HA complex was found to be upregulated in patients at various clinical stages of chronic hepatitis (CH), liver cirrhosis (LC), and hepatocellular carcinoma (HCC) caused by infection with the hepatitis C or hepatitis B virus (7).  ITIH1 has been shown to increase cell attachment and reduce the number of metastases in an antitumoral role (8).


  1. Salier, J.P. et al. (1996) Biochem. J. 315:1.
  2. Jean, L. et al. (1997) Genomics 41:139.
  3. Zhuo, L. et al. (2004) J. Biol. Chem. 279:38079.
  4. Rugg, M.S. et al. (2005) J. Biol. Chem. 280:25674.
  5. Baranova, N.S et al. (2013) J. Biol. Chem. 288: 29642.
  6. Zhuo, L. et al. (2006) J. Biol. Chem. 281:20303.
  7. Shen, L. et al.  (2006) Hepatol. Res. 34:178.
  8. Paris, S. et al. (2002) Int. J. Cancer 97:615.

Long Name

Inter-Alpha-Trypsin Inhibitor Heavy Chain 1

Alternate Names


Entrez Gene IDs

3697 (Human); 16424 (Mouse)

Gene Symbol



Additional ITIH1 Products

Product Documents for Recombinant Human ITIH1 His-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human ITIH1 His-tag Protein, CF

For research use only

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.