Recombinant Human Integrin alpha 6 (X1) beta 4 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 5497-A6

R&D Systems, part of Bio-Techne
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5497-A6-050
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Key Product Details

Source

CHO

Accession #

NP_000201 (Integrin alpha6) & P16144 (Integrin beta4)

Conjugate

Unconjugated

Applications

Bioactivity

View all Integrin alpha 6 beta 4 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Integrin alpha 6 beta 4 protein
Human Integrin alpha6
(Phe24-Lys878)
Accession # NP_000201		 Acidic Tail HHHHHH
Human Integrin beta4
(Asn28-Ser710)
Accession # P16144		 Basic Tail
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe24 ( alpha6) & Asn28 ( beta4)

Predicted Molecular Mass

104 kDa ( alpha6) & 84.8 kDa ( beta4)

SDS-PAGE

125-135 kDa ( alpha6) & 100-110 kDa ( beta4), reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Mouse Laminin I (Catalog # 3400-010-02) is coated at 10 μg/mL, Recombinant Human Integrin alpha6 beta4 binds with an apparent KD <5 nM.

Reviewed Applications

Read 3 reviews rated 4.3 using 5497-A6 in the following applications:

Formulation, Preparation and Storage

5497-A6
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 200 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 6 beta 4

Integrin alpha6 beta4 is an epithelial and Schwann cell laminin-binding integrin. While alpha6 can also pair with beta1, beta4 pairs only with alpha6 (1-3). Expression of the non-covalent heterodimer composed of a ~150 kDa alpha6/CD49f and 150-200 kDa beta4/CD104 type I transmembrane glycoprotein subunit is required for hemidesmosome formation (2, 4). The alpha6 subunit contains an I (inhibitory) domain and a cleavage site that creates extracellular heavy and transmembrane light chains. Alternative splicing in the alpha6 extracellular domain (ECD) at amino acid (aa) 216 creates X1 (ubiquitous), X2 and X1X2 isoforms, while splicing at a cytoplasmic site creates A and B isoforms (5, 6). The cytoplasmic domain of beta4 is unusually long (~1000 aa) and contains four type III fibronectin repeats that bind intracellular hemidesmosomal components (2, 4, 7). Alternative splicing between repeats # 2 and 3 creates A, B, C and (truncated) D isoforms (7). All ECD variants share similar ligand binding characteristics, while cytoplasmic variants differ in tissue distribution and signaling pathways (5-7). The 683 aa human alpha6X1 heavy chain shares 94-95% aa identity with mouse, rat, bovine, and canine a6, and the 683 aa human beta4 ECD shares 87-92% aa identity with mouse, rat, bovine, and equine beta4. Mutation of alpha6 beta4 can cause EB-PA, or epidermolysis bullosa (detachment of epidermis from basement membrane) with pyloric atresia, that is neonatally lethal if severe (8). On Schwann cells, alpha6 beta4 cooperates with dystroglycan to stabilize the myelin sheath, and mediates attachment to the basal lamina (9, 10). High alpha6 beta4 expression correlates with invasiveness of carcinomas (2). EGF R-induced phosphorylation of beta4 disrupts hemidesmosomes and allows tumor cell migration (11, 12). alpha6 beta4 signaling can also amplify tumor production of VEGF and ErbB proteins (13, 14).

References

  1. Takada, Y. et al. (2007) Genome Biol. 8:215.
  2. Wilhelmsen, K. et al. (2006) Mol. Cell. Biol. 26:2877.
  3. Nishiuchi, R. et al. (2006) Matrix Biol. 25:189.
  4. Schaapveld, R. Q. J. et al. (1998) J. Cell Biol. 142:271.
  5. Delwel, G. O. et al. (1995) Cell Adhes. Commun. 3:143.
  6. Hogervorst, F. et al. (1993) J. Cell Biol. 121:179.
  7. van Leusden, M. R. et al. (1997) Biochem. Biophys. Res. Commun. 235:826.
  8. Pulkkinen, L. et al. (1998) Am. J. Hum. Genet. 63:1376.
  9. Nodari, A. et al. (2008) J. Neurosci. 28:6714.
  10. Amici, S. A. et al. (2006) J. Neurosci. 26:1179.
  11. Wilhelmsen, K. et al. (2007) Mol. Biol. Cell 18:3512.
  12. Yang, X. H. et al. (2008) Cancer Res. 68:3204.
  13. Lipscomb, E. A. et al. (2005) Cancer Res. 65:10970.
  14. Folgiero, V. et al. (2007) Cancer Res. 67:1645.

Entrez Gene IDs

3655 (Human); 16407 (Mouse)

Gene Symbol

ITGA6

UniProt

NP_000201 (Integrin alpha6) & P16144 (Integrin beta4)

Additional Integrin alpha 6 beta 4 Products

Product Documents for Recombinant Human Integrin alpha 6 (X1) beta 4 Protein, CF

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Product Specific Notices for Recombinant Human Integrin alpha 6 (X1) beta 4 Protein, CF

For research use only

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