Product Specifications for Recombinant Human IL1RAPL2 His-tag Protein, CF
Chinese Hamster Ovary cell line, CHO-derived human IL1RAPL2 protein Thr17-Glu356, with a C-terminal 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Thr17 and Lys20
Predicted Molecular Mass
40-60 kDa, under reducing conditions.
Measured by its binding ability in a functional ELISA.
When Recombinant Human IL1RAPL2 His-tag (Catalog # 11207-MR) is immobilized at 2 µg/mL (100 µL/well), Recombinant Human PTPRD Fc Chimera (Catalog # 9995-PR) binds with an ED50 of 45.0-450 ng/mL.
Scientific Data Examples for Recombinant Human IL1RAPL2 His-tag Protein, CF
Recombinant Human IL1RAPL2 His-tag Protein Binding Activity.
When Recombinant Human IL1RAPL2 His-tag Protein (Catalog # 11207-MR) is immobilized at 2 µg/mL (100 µL/well), Recombinant Human PTPRD Fc Chimera Protein (9995-PR) binds with an ED50 of 45.0-450 ng/mL.
Recombinant Human IL1RAPL2 His-tag Protein SDS-PAGE.
2 μg/lane of Recombinant Human IL1RAPL2 His-tag Protein (Catalog # 11207-MR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 40-60 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitute at 500 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
The Interleukin 1 receptor family (IL-1R) comprises at least eleven members including IL-1 RI (IL-1R1), IL-1 RII (IL-1 R2), IL-1 RAcP (IL-1 R3), ST2 (T1/IL‑1 R4), IL‑18 R alpha (IL-1 Rrp/IL-1 R5), IL-1 Rrp2 (IL-1 RL2/IL-1 R6), IL-18 R beta (AcPL/IL-1 R7), IL1RAPL1 (TIGIRR-2/IL-1 R8), and IL1RAPL2 (TIGIRR-1/IL-1 R9) (1). All family members possess three immunoglobulin (Ig)-like domains in their extracellular region. Most members also have an intracellular TIR (Toll-like receptor/IL-1 receptor signaling) domain that is also conserved in the Toll-like receptor family. Related proteins, SIGIRR (single Ig domain-containing IL-1 R-related molecule) and IL-18BP, differ from the other members by having only one Ig domain (1). IL-1 receptor accessory protein-like 2 (IL1RAPL2) is alternately known as IL-1 R9 and three immunoglobulin domain containing IL-1 receptor-related molecule 1 (TIGIRR-1) and is expressed in the brain (2). Its sequence predicts an 686 amino acid (aa) residue type I transmembrane glycoprotein with a 17 aa signal peptide, a 339 aa extracellular region containing three Ig-like domains, an 18 aa transmembrane domain and a 312 aa cytoplasmic tail (3). By comparison to other IL-1 receptor family proteins, IL1RAPL2 has a C-terminal cytoplasmic extension beyond the TIR domain that is found in IL-1RAPL1 and SIGIRR but not other family members (3). Human and mouse IL1RAPL2 share approximately 95% aa sequence identity. Human IL1RAPL2 is most homologous (63%) to IL-1RAPL1, a receptor protein that is highly expressed in hippocampus and is involved in X-linked mental retardation (4, 5). Genes for both have been localized to human chromosome Xq22. A ligand for IL1RAPL2 has not been identified (1).
Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
Andre, R. et al. (2005) J. Neurochem. 95:324.
Born, T.L. et al. (2000) J. Biol. Chem. 275:29946.
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