Product Specifications for Recombinant Human IL1RAPL1 His-tag Protein, CF
Human embryonic kidney cell, HEK293-derived human IL1RAPL1 protein Leu19-Thr357, with a C-terminal 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
42-59 kDa, under reducing conditions.
Measured by its binding ability in a functional ELISA. When Recombinant Human IL1RAPL1 His-tag (Catalog # 11080-MR) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Human PTPRD Fc Chimera (Catalog # 9995-PR) binds with an ED50 of 6.00-60.0 ng/mL.
Scientific Data Examples for Recombinant Human IL1RAPL1 His-tag Protein, CF
Recombinant Human IL1RAPL1 His-tag Protein Binding Activity.
When Recombinant Human IL1RAPL1 His-tag Protein (Catalog # 11080-MR) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Human PTPRD Fc Chimera (9995-PR) binds with an ED50 of 6.00-60.0 ng/mL.
Recombinant Human IL1RAPL1 His-tag Protein SDS-PAGE.
2 μg/lane of Recombinant Human IL1RAPL1 His-tag Protein (Catalog # 11080-MR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 42-59 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitute at 500 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Interleukin 1 receptor accessory protein-like 1 (IL1RAPL1), also known as Oligophenin-4 (OPHN4) and three immunoglobulin domain containing IL-1 receptor-related 2 (TIGIRR-2) (1), is a member of the IL-1 receptor superfamily. IL1RAPL1 is a single pass type I membrane protein which contains an N-terminal signal peptide (aa 1-18 ), three extracellular immunoglobulin-like domains (aa 19-350), a transmembrane domain (aa 358-378 ), an intracellular Toll/IL-1R domain (aa 403-562), and a long C-terminal tail which interacts with multiple signaling molecules (aa 549-644 ) (2). High expression levels of IL1RAPL1 was found in post-natal hippocampus, and its expression is upregulated by neuronal activity (3). The extracellular domain of IL1RAPL1 can mediate synapse formation through trans-synaptic interaction with PTPRD (4, 5). In neurons, IL1RAPL1 interacts with PSD-95, a major scaffolding protein of excitatory synapses, and modulates its synaptic localization by regulating JNK activity and PSD-95 phosphorylation (3). Mutation or deletion of IL1RAPL1 gene is associated with non-syndromic intellectual disability and autism spectrum disorder (5). Human IL1RAPL1 shares 98% and 99% aa sequence identity with mouse and rat IL1RAPL1, respectively.
Born, T.L. et al. (2000) J. Biol. Chem. 275:29946.
Bahi, N. et al. (2003) Hum. Mol. Gen. 12:1415.
Pavlowsky, A. et al. (2010) Curr. Biol. 20:103.
Yoshida, T. et al. (2011) J. Neurosci. 31:13485.
Ramos-Brossier, M. et al. (2015) Hum Mol Genet. 24:1106.
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