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Recombinant Human IL-9R (NS0-expressed) Protein

Catalog # 290-RNS | R&D Systems, Inc. a Bio-Techne Brand
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Carrier Free
290-RNS-025/CF

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With Carrier
290-RNS-025

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human IL-9 R protein
Val40-Pro270, with a C-terminal 10-His tag

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val40

Predicted Molecular Mass

27.4 kDa

SDS-PAGE

38 kDa, reducing conditions

Activity

Measured by its ability to inhibit the IL-9-dependent proliferation of MO7e human megakaryocytic leukemic cells.
The ED50 for this effect is 1-4 µg/mL in the presence of 2 ng/mL of recombinant human IL-9.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 290-RNS
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with BSA as a carrier protein.
Reconstitution Reconstitute at 250 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 290-RNS/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
Reconstitution Reconstitute at 250 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-9R

The specific high affinity functional IL-9 receptor complex consists of a ligand-specific IL-9 R( alpha) subunit and the common gamma chain that is shared with the receptors for IL-2, IL-4, IL-7 and IL-15. Both the IL-9 R subunit and the common gamma chain are members of the hematopoietin receptor superfamily. The cDNAs encoding mouse and human IL-9 receptors have been isolated. The deduced mouse and human transmembrane proteins, sharing 53% amino acid sequence identity, contain 468 and 533 amino acid residues, respectively. As a result of alternative splicing, cDNA clones encoding isoforms of IL-9 R, including a putative soluble form, have also been identified. Cells known to express IL-9 R include T cells, neutrophils, mast cells and macrophages.

References

  1. Renauld, J. et al. (1992) Proc. Natl. Acad. Sci. USA, 89:5690.
  2. Kimura, Y. et al. (1995) Int. Immunol. 7:115.

Long Name

Interleukin 9 Receptor

Alternate Names

CD129, IL-9 R, IL9R

Entrez Gene IDs

3581 (Human); 16199 (Mouse)

Gene Symbol

IL9R

UniProt

Product Documents for Recombinant Human IL-9R (NS0-expressed) Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human IL-9R (NS0-expressed) Protein

For research use only

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