Recombinant Human IL-7R alpha/CD127 His-tag Protein, CF

Catalog # 10758-IR | R&D Systems, Inc. a Bio-Techne Brand
Extracellular Domain. HEK293 Expressed
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Key Product Details

Accession #

AAC83204.1

Source

HEK293

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human IL-7R alpha/CD127 protein
Glu21-Asp239, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu21

Predicted Molecular Mass

26 kDa

SDS-PAGE

44-51 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
In a His Tag Antibody (Catalog # MAB050R) coated plate, when Recombinant Human IL-7R alpha/CD127 His-tag is present at 500 ng/mL, Recombinant Human IL-7 (Catalog # 207-IL) binds with an ED50 of 0.75-4.5 ng/mL.

Scientific Data Images for Recombinant Human IL-7R alpha/CD127 His-tag Protein, CF

Recombinant Human IL-7R alpha/CD127 His-tag Protein Binding Activity.

In a His Tag Antibody (MAB050R) coated plate, when Recombinant Human IL-7R alpha/CD127 His-tag (Catalog # 10758-IR) is present at 500 ng/mL, Recombinant Human IL-7 (207-IL) binds with an ED50 of 0.75-4.5 ng/mL.

Recombinant Human IL-1R alpha/CD127 His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human IL-7R alpha/CD127 His-tag (Catalog # 10758-IR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 44-51 kDa.

Formulation, Preparation and Storage

10758-IR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-7R alpha/CD127

Interleukin 7 Receptor alpha (IL-7 R alpha), also known as CD127, is a 75 kDa hematopoietin receptor superfamily member that plays an important role in lymphocyte differentiation, proliferation, and survival (1, 2). Mature human IL-7 R alpha consists of a 219 amino acid (aa) extracellular domain (ECD) with one fibronectin type-III domain and a WSXWS motif, a 25 aa transmembrane segment, and a 195 aa cytoplasmic domain (3). Alternate splicing of human IL-7 R alpha generates a secreted soluble form of the receptor (3). Within the ECD, human IL‑7 R alpha shares 67% aa sequence identity with mouse and rat IL-7 R alpha. IL-7 R alpha associates with the common gamma chain ( gammac) to form the functional high affinity IL-7 receptor complex (4). The gammac is also a subunit of the receptors for IL-2, -4, -9, -15, and -21. Human and mouse IL‑7 show cross-species activity through the IL-7 receptor (3, 5). IL-7 R alpha is expressed on double negative (CD44-/CD8+) and CD4+ or CD8+ single positive T cells as well as on CD8+ memory T cells and their precursors (6, 7). It is expressed early in B cell development, prior to the appearance of surface IgM (6). In mouse, IL-7 activation of IL-7 R alpha is critical for both T cell and B cell lineage development (8). In human, by contrast, it is required for T cell but not for B cell development (9). IL-7 induces the downregulation and shedding of cell surface IL‑7 R alpha (10). IL-7 R alpha additionally associates with TSLP R to form the functional receptor for thymic stromal lymphopoietin (11, 12). TSLP indirectly regulates T cell development by modulating dendritic cell activation (2, 13). Knockout of TSLP R in mice provokes minor changes in B and T cell development compared to those seen with IL-7 R alpha deletion (8, 14). The complexity of IL-7 R alpha biology is suggested by the competition between IL-7 and TSLP for receptor binding and by the ability of IL-7 R alpha to form functional complexes with SCF R and HGF R (11, 12, 15, 16).

References

  1. Mazzucchelli, R. and S.K. Durum (2007) Nat. Rev. Immunol. 7:144.
  2. Liu, Y.-J. et al. (2007) Annu. Rev. Immunol. 25:193.
  3. Goodwin, R.G. et al. (1990) Cell 60:941.
  4. Noguchi, M. et al. (1993) Science 262:1877.
  5. Barata, J.T. et al. (2006) Exp. Hematol. 34:1133.
  6. Sudo, T. et al. (1993) Proc. Natl. Acad. Sci. 90:9125.
  7. Kaech, S.M. et al. (2003) Nat. Immunol. 4:1191.
  8. Peschon, J.J. et al. (1994) J. Exp. Med. 180:1955.
  9. Prieyl, J.A. and T.W. LeBien (1996) Proc. Natl. Acad. Sci. 93:10348.
  10. Vranjkovic, A. et al. (2007) Int. Immunol. 19:1329.
  11. Park, L.S. et al. (2000) J. Exp. Med. 192:659.
  12. Pandey, A. et al. (2000) Nat. Immunol. 1:59.
  13. Reche, P.A. et al. (2001) J. Immunol. 167:336.
  14. Al-Shami, A. et al. (2004) J. Exp. Med. 200:159.
  15. Jahn, T. et al. (2007) Blood 110:1840.
  16. Lai, L. et al. (2006) Blood 107:1776.

Long Name

Interleukin 7 Receptor alpha

Alternate Names

CD127, IL-7 R alpha, IL-7Ra, IL7R, IL7R alpha

Entrez Gene IDs

3575 (Human); 16197 (Mouse); 294797 (Rat); 102143679 (Cynomolgus Monkey)

Gene Symbol

IL7R

UniProt

AAC83204.1

Product Documents for Recombinant Human IL-7R alpha/CD127 His-tag Protein, CF

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Product Specific Notices for Recombinant Human IL-7R alpha/CD127 His-tag Protein, CF

For research use only

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