Recombinant Human IL-7R alpha/CD127 His-tag Protein, CF

Name: Recombinant Human IL-7R alpha/CD127 His-tag Protein, CF
Brand: R&D Systems
SKU: 10758-IR

Catalog # 10758-IR

Product Datasheet / COA / SDS

Extracellular Domain. HEK293 Expressed

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Key Product Details

Source:	HEK293
Accession #:	AAC83204.1
Applications:	Bioactivity

View Full Technical Details for Recombinant Human IL-7R alpha/CD127 His-tag Protein, CF

Product Specifications

Source	Human embryonic kidney cell, HEK293-derived human IL-7R alpha/CD127 protein Glu21-Asp239, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Glu21
Predicted Molecular Mass	26 kDa
SDS-PAGE	44-51 kDa, under reducing conditions
Activity	Measured by its binding ability in a functional ELISA. In a His Tag Antibody (Catalog # MAB050R) coated plate, when Recombinant Human IL-7R alpha/CD127 His-tag is present at 500 ng/mL, Recombinant Human IL-7 (Catalog # 207-IL) binds with an ED₅₀ of 0.75-4.5 ng/mL.

Recombinant Human IL-7R alpha/CD127 His-tag Protein, CF Scientific Data Examples

	Recombinant Human IL-7R alpha/CD127 His-tag Protein Binding Activity. In a His Tag Antibody (MAB050R) coated plate, when Recombinant Human IL-7R alpha/CD127 His-tag (Catalog # 10758-IR) is present at 500 ng/mL, Recombinant Human IL-7 (207-IL) binds with an ED₅₀ of 0.75-4.5 ng/mL.
	Recombinant Human IL-1R alpha/CD127 His-tag Protein SDS-PAGE. 2 μg/lane of Recombinant Human IL-7R alpha/CD127 His-tag (Catalog # 10758-IR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 44-51 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10758-IR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-7R alpha/CD127

Interleukin 7 Receptor alpha (IL-7 R alpha ), also known as CD127, is a 75 kDa hematopoietin receptor superfamily member that plays an important role in lymphocyte differentiation, proliferation, and survival (1, 2). Mature human IL-7 R alpha consists of a 219 amino acid (aa) extracellular domain (ECD) with one fibronectin type-III domain and a WSXWS motif, a 25 aa transmembrane segment, and a 195 aa cytoplasmic domain (3). Alternate splicing of human IL-7 R alpha generates a secreted soluble form of the receptor (3). Within the ECD, human IL‑7 R alpha shares 67% aa sequence identity with mouse and rat IL-7 R alpha. IL-7 R alpha associates with the common gamma chain ( gamma c) to form the functional high affinity IL-7 receptor complex (4). The gamma _c is also a subunit of the receptors for IL-2, -4, -9, -15, and -21. Human and mouse IL‑7 show cross-species activity through the IL-7 receptor (3, 5). IL-7 R alpha is expressed on double negative (CD44-/CD8+) and CD4+ or CD8+ single positive T cells as well as on CD8+ memory T cells and their precursors (6, 7). It is expressed early in B cell development, prior to the appearance of surface IgM (6). In mouse, IL-7 activation of IL-7 R alpha is critical for both T cell and B cell lineage development (8). In human, by contrast, it is required for T cell but not for B cell development (9). IL-7 induces the downregulation and shedding of cell surface IL‑7 R alpha (10). IL-7 R alpha additionally associates with TSLP R to form the functional receptor for thymic stromal lymphopoietin (11, 12). TSLP indirectly regulates T cell development by modulating dendritic cell activation (2, 13). Knockout of TSLP R in mice provokes minor changes in B and T cell development compared to those seen with IL-7 R alpha deletion (8, 14). The complexity of IL-7 R alpha biology is suggested by the competition between IL-7 and TSLP for receptor binding and by the ability of IL-7 R alpha to form functional complexes with SCF R and HGF R (11, 12, 15, 16).

Item	Value
References	Mazzucchelli, R. and S.K. Durum (2007) Nat. Rev. Immunol. 7:144. Liu, Y.-J. et al. (2007) Annu. Rev. Immunol. 25:193. Goodwin, R.G. et al. (1990) Cell 60:941. Noguchi, M. et al. (1993) Science 262:1877. Barata, J.T. et al. (2006) Exp. Hematol. 34:1133. Sudo, T. et al. (1993) Proc. Natl. Acad. Sci. 90:9125. Kaech, S.M. et al. (2003) Nat. Immunol. 4:1191. Peschon, J.J. et al. (1994) J. Exp. Med. 180:1955. Prieyl, J.A. and T.W. LeBien (1996) Proc. Natl. Acad. Sci. 93:10348. Vranjkovic, A. et al. (2007) Int. Immunol. 19:1329. Park, L.S. et al. (2000) J. Exp. Med. 192:659. Pandey, A. et al. (2000) Nat. Immunol. 1:59. Reche, P.A. et al. (2001) J. Immunol. 167:336. Al-Shami, A. et al. (2004) J. Exp. Med. 200:159. Jahn, T. et al. (2007) Blood 110:1840. Lai, L. et al. (2006) Blood 107:1776.
Long Name	Interleukin 7 Receptor alpha
Entrez Gene IDs	3575 (Human); 16197 (Mouse); 294797 (Rat); 102143679 (Cynomolgus Monkey)
Alternate Names	CD127, CD127 antigen, CD127ILRA, CDW127, IL-7 R alpha, IL-7 receptor subunit alpha, IL-7R alpha, IL-7R subunit alpha, IL-7R-alpha, IL-7Ra, IL7R, IL7R alpha, IL7RA, interleukin 7 receptor, interleukin 7 receptor alpha chain, interleukin 7 receptor isoform H5-6, interleukin-7 receptor subunit alpha

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