Recombinant Human IL-7R alpha/CD127 Fc Chimera Protein, CF

Name: Recombinant Human IL-7R alpha/CD127 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 10531-IR

Catalog # 10531-IR

Product Datasheet / COA / SDS

Extracellular domain

Order Information

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Key Product Details

Source:	CHO
Accession #:	P16871.2
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human IL-7R alpha/CD127 Fc Chimera Protein, CF

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human IL-7R alpha/CD127 protein

Human IL-7R alpha/CD127 (Gln21-Asp239) Accession # P16871.2	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Glu21

Predicted Molecular Mass

54 kDa

SDS-PAGE

78-100 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA. In the presence of 100 ng/mL of Recombinant Human IL-7 (Catalog # 207-IL), Recombinant Human IL-7R alpha/CD127 (Extracellular Domain) Fc Chimera (Catalog # 10531-IR) binds with an ED₅₀ of 5-30 ng/mL.

Recombinant Human IL-7R alpha/CD127 Fc Chimera Protein, CF Scientific Data Examples

	Recombinant Human IL-7R alpha/CD127 Fc Chimera Protein Binding Activity In the presence of 100 ng/mL of Recombinant Human IL-7 (207-IL), Recombinant Human IL-7R alpha/CD127 (Extracellular Domain) Fc Chimera Protein (Catalog # 10531-IR) binds with an ED₅₀ of 5-30 ng/mL.
	Recombinant Human IL-7R alpha/CD127 Fc Chimera Protein SDS-PAGE 2 μg/lane of Recombinant Human IL-7R alpha/CD127 Fc Chimera Protein (10531-IR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 78-100 kDa and 156-200 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10531-IR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Desired Reconstitution Concentration

Background: IL-7R alpha/CD127

Interleukin 7 Receptor alpha (IL-7 R alpha ), also known as CD127, is a 75 kDa hematopoietin receptor superfamily member that plays an important role in lymphocyte differentiation, proliferation, and survival (1, 2). Mature human IL-7 R alpha consists of a 219 amino acid (aa) extracellular domain (ECD) with one fibronectin type-III domain and a WSXWS motif, a 25 aa transmembrane segment, and a 195 aa cytoplasmic domain (3). Alternate splicing of human IL-7 R alpha generates a secreted soluble form of the receptor (3). Within the ECD, human IL‑7 R alpha shares 67% aa sequence identity with mouse and rat IL-7 R alpha. IL-7 R alpha associates with the common gamma chain ( gamma _c) to form the functional high affinity IL-7 receptor complex (4). The gamma _c is also a subunit of the receptors for IL-2, -4, -9, -15, and -21. Human and mouse IL‑7 show cross-species activity through the IL-7 receptor (3, 5). IL-7 R alpha is expressed on double negative (CD4^-/CD8^-) and CD4⁺ or CD8⁺ single positive T cells as well as on CD8⁺ memory T cells and their precursors (6, 7). It is expressed early in B cell development, prior to the appearance of surface IgM (6). In mouse, IL-7 activation of IL-7 R alpha is critical for both T cell and B cell lineage development (8). In human, by contrast, it is required for T cell but not for B cell development (9). IL-7 induces the downregulation and shedding of cell surface IL‑7 R alpha (10). IL-7 R alpha additionally associates with TSLP R to form the functional receptor for thymic stromal lymphopoietin (11, 12). TSLP indirectly regulates T cell development by modulating dendritic cell activation (2, 13). Knockout of TSLP R in mice provokes minor changes in B and T cell development compared to those seen with IL-7 R alpha deletion (8, 14). The complexity of IL-7 R alpha biology is suggested by the competition between IL-7 and TSLP for receptor binding and by the ability of IL-7 R alpha to form functional complexes with SCF R and HGF R (11, 12, 15, 16).

Item	Value
References	Mazzucchelli, R. and S.K. Durum (2007) Nat. Rev. Immunol. 7:144. Liu, Y.-J. et al. (2007) Annu. Rev. Immunol. 25:193. Goodwin, R.G. et al. (1990) Cell 60:941. Noguchi, M. et al. (1993) Science 262:1877. Barata, J.T. et al. (2006) Exp. Hematol. 34:1133. Sudo, T. et al. (1993) Proc. Natl. Acad. Sci. 90:9125. Kaech, S.M. et al. (2003) Nat. Immunol. 4:1191. Peschon, J.J. et al. (1994) J. Exp. Med. 180:1955. Prieyl, J.A. and T.W. LeBien (1996) Proc. Natl. Acad. Sci. 93:10348. Vranjkovic, A. et al. (2007) Int. Immunol. 19:1329. Park, L.S. et al. (2000) J. Exp. Med. 192:659. Pandey, A. et al. (2000) Nat. Immunol. 1:59. Reche, P.A. et al. (2001) J. Immunol. 167:336. Al-Shami, A. et al. (2004) J. Exp. Med. 200:159. Jahn, T. et al. (2007) Blood 110:1840. Lai, L. et al. (2006) Blood 107:1776.
Long Name	Interleukin 7 Receptor alpha
Entrez Gene IDs	3575 (Human); 16197 (Mouse); 294797 (Rat); 102143679 (Cynomolgus Monkey)
Alternate Names	CD127, CD127 antigen, CD127ILRA, CDW127, IL-7 R alpha, IL-7 receptor subunit alpha, IL-7R alpha, IL-7R subunit alpha, IL-7R-alpha, IL-7Ra, IL7R, IL7R alpha, IL7RA, interleukin 7 receptor, interleukin 7 receptor alpha chain, interleukin 7 receptor isoform H5-6, interleukin-7 receptor subunit alpha

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