Recombinant Human IL-6/IL-6R alpha Protein Chimera

Name: Recombinant Human IL-6/IL-6R alpha Protein Chimera
Brand: R&D Systems
SKU: 8954-SR
Rating: 4 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 8954-SR

Citations (6)

(1)

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Setting the standard in quality research reagents

Key Product Details

Source:	HEK293
Accession #:	P08887 (IL-6 R alpha) & P05231 (IL-6)
Applications:	Bioactivity

View Full Technical Details for Recombinant Human IL-6/IL-6R alpha Protein Chimera

Product Specifications for Recombinant Human IL-6/IL-6R alpha Protein Chimera

Source

Human embryonic kidney cell, HEK293-derived human IL-6/IL-6R alpha Complex protein

Human IL-6R alpha (Leu20-Asp358) Accession # P08887	GGGSGGGSGGGS	Human IL-6 Val30-Met212 Accession # P05231
N-terminus		C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Leu20 (Human IL-6R alpha)

Predicted Molecular Mass

59 kDa

SDS-PAGE

78-93 kDa, reducing conditions

Activity

Measured by its ability to inhibit M1 mouse myeloid leukemia cell proliferation. The ED₅₀ for this effect is 2.5-12.5 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

8954-SR

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

8954-SR/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-6/IL-6R alpha Complex

Interleukin-6 (IL-6) is a pleiotropic, alpha -helical, 22-28 kDa phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression (1-5). Mature human IL-6 is 183 amino acids (aa) in length and shares 39% aa sequence identity with mouse and rat IL-6 (6). Alternative splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties (7-10). IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R alpha) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R alpha, triggering IL-6 R alpha association with gp130 and gp130 dimerization (11). gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (12). Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage (5). In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha (5). Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R alpha is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes (2, 5). Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R alpha but not from other cytokines that use gp130 as a co-receptor (5, 13). IL-6, along with TNF-alpha and IL-1, drives the acute inflammatory response and the transition from acute inflammation to either acquired immunity or chronic inflammatory disease (1-5). When dysregulated, it contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis (1, 2, 5). IL-6 can also function as an anti-inflammatory molecule, as in skeletal muscle where it is secreted in response to exercise (2). In addition, it enhances hematopoietic stem cell proliferation and the differentiation of Th17 cells, memory B cells, and plasma cells (1, 14).

Item	Value
References	Mansell, A. and B.J. Jenkins (2013) Cytokine Growth Factor Rev. 24:249. Schuett, H. et al. (2009) Thromb. Haemost. 102:215. Erta, M. et al. (2012) Int. J. Biol. Sci. 8:1254. Garbers, C. et al. (2012) Cytokine Growth Factor Rev. 23:85. Mihara, M. et al. (2012) Clin. Sci. (Lond.) 122:143. Hirano, T. et al. (1986) Nature 324:73. Kestler, D.P. et al. (1995) Blood 86:4559. Kestler, D.P. et al. (1999) Am. J. Hematol. 61:169. Bihl, M.P. et al. (2002) Am. J. Respir. Cell Mol. Biol. 27:48. Alberti, L. et al. (2005) Cancer Res. 65:2. Murakami, M. et al. (1993) Science 260:1808. Muller-Newen, G. (2003) Sci. STKE 2003:PE40. Mitsuyama, K. et al. (2006) Clin. Exp. Immunol. 143:125. Cerutti, A. et al. (1998) J. Immunol. 160:2145.
Long Name	Interleukin 6/Interleukin 6 Receptor alpha Complex
Entrez Gene IDs	3570 (Human); 16194 (Mouse); 24499 (Rat)
Alternate Names	IL-6/IL-6R alpha Complex

Citations for Recombinant Human IL-6/IL-6R alpha Protein Chimera (6)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human IL-6/IL-6R alpha Protein Chimera include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Z Cai et al. (2022), Kynurenine metabolites predict survival in pulmonary arterial hypertension: A role for IL-6/IL-6Ralpha Scientific Reports, 12(1):12326.
PMID: 35853948
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

K Leng et al. (2022), CRISPRi screens in human iPSC-derived astrocytes elucidate regulators of distinct inflammatory reactive states Nature Neuroscience, 25(11):1528-1542.
PMID: 36303069
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

J Dong et al. (2021), Hepatocyte-specific IL11 cis-signaling drives lipotoxicity and underlies the transition from NAFLD to NASH Nature Communications, 12(1):66.
PMID: 33397952
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

G Carbotti et al. (2021), IL-27 Mediates PD-L1 Expression and Release by Human Mesothelioma Cells Cancers, 13(16).
PMID: 34439164
Species: Human
Sample Types: cell culture
Applications: Bioassay

T Kretschmer et al. (2020), Effect of Maternal Obesity in Mice on IL-6 Levels and Placental Endothelial Cell Homeostasis Nutrients, 12(2).
PMID: 31979004
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Ohta R et al. (0), Hemogenic Endothelium Differentiation from Human Pluripotent Stem Cells in A Feeder- and Xeno-free Defined Condition. J Vis Ex0(148).
PMID: 31259914

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Recombinant Human IL-6/IL-6 R alpha Protein Chimera

Name: Anna Wang

Application: Apoptosis assay

Verified Customer | Posted 12/01/2017

Recombinant Human IL-6/IL-6R alpha Protein Chimera

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Human IL-6/IL-6R alpha Protein Chimera

Formulation, Preparation and Storage

Carrier Free

8954-SR

Carrier

8954-SR/CF

Carrier Free

Reconstitution Calculator

Background: IL-6/IL-6R alpha Complex

Citations for Recombinant Human IL-6/IL-6R alpha Protein Chimera (6)

Customer Reviews for Recombinant Human IL-6/IL-6R alpha Protein Chimera (1)

Recombinant Human IL-6/IL-6 R alpha Protein Chimera

Product Documents for Recombinant Human IL-6/IL-6R alpha Protein Chimera

Product Datasheets

COA

Certificate of Analysis

SDS

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