Recombinant Human IL-37b/IL-1F7b Protein

Name: Recombinant Human IL-37b/IL-1F7b Protein
Brand: R&D Systems
SKU: 7585-IL

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 7585-IL

Citations (9)

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rh IL-37 Isoform a (Catalog # 1975-IL) is also available!

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Key Product Details

Source:	E. coli
Accession #:	Q9NZH6
Applications:	Bioactivity

View Full Technical Details for Recombinant Human IL-37b/IL-1F7b Protein

Product Specifications for Recombinant Human IL-37b/IL-1F7b Protein

Source	E. coli-derived human IL-37b/IL-1F7b protein Val46-Asp218
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Val46
Predicted Molecular Mass	19.4 kDa
SDS-PAGE	25 kDa, reducing conditions
Activity	Measured by its ability to enhance IL-18 BP inhibition of IL-18-induced IFN-gamma release by KG‑1 human acute myelogenous leukemia cells. Bufler, P. et al. (2002) PNAS 99:13723. The ED₅₀ for this effect is 2.5-12.5 μg/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

7585-IL

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS and DTT with BSA as a carrier protein.
Reconstitution	Reconstitute at 200 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

7585-IL/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS and DTT.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: IL-37b/IL-1F7b

Human Interleukin 1 family member 7 (IL-1F7), also named FIL-1 zeta, IL-37, IL-1H4, IL-1HL and IL-1RP1, is an anti-inflammatory member of the IL-1 cytokine family. IL-1F7 shares approximately 21%, 24%, and 30% aa sequence identity with mature IL-1 alpha, IL-1 beta and IL-1ra, respectively. Mouse IL-1F7 has not been reported, but human IL-1F7 is active on mouse cells. Five isoforms (IL-1F7a through e) are generated through alternative exon usage and have distinct expression profiles (1‑5). Like IL-1 alpha, IL-1 beta and IL-18, all of the IL-1F7 variants lack a typical signal peptide. The longest IL-1F7 transcript is the basis for the product offered here. This product, also referred to as IL-1F7b, or IL-1F7 isoform 1, is normally generated from a 218 amino acid (aa) proprotein that consists of a 45 aa propeptide plus a 173 aa mature region that is cleaved by caspase-1 to generate the 32 kDa mature form (aa 46-218) (6). IL-1F7 is up-regulated by inflammatory stimuli in peripheral blood mononuclear cells (7). Experimental overexpression of IL-1F7b in vivo limits the inflammatory response and protects mice from colitis and LPS-induced shock (7, 8). Both unprocessed and mature IL-1F7b can form homodimers in solution (6, 7). Although IL-1F7b will bind to IL-18 R alpha with low affinity, this has no effect on IL‑18 receptor activity (4, 6, 9). Alternatively, IL-1F7b will also bind to IL-18 BP, generating a complex that interacts with IL-18R beta. This has the effect of attenuating IL‑18 activity via the IL-18 receptor (9). IL-1F7b can also function intracellularly. Following LPS-induced cleavage of its propeptide, IL-1F7b associates with Smad3 and translocates to the nucleus. This results in a reduction of pro-inflammatory cytokine secretion (10).

Item	Value
References	Smith, D.E. et al. (2000) J. Biol Chem 275:1169. Kumar S. et al. (2000) J. Biol Chem 275:10308. Busfield S.J. et al. (2000) Genomics 66:213. Pan, G. et al. (2000) Cytokine 13:1. Boraschi, D. et al. (2011) Eur. Cytokine Netw. 22:127. Kumar S. et al. (2002) Cytokine 18:61. Nold, M.F. et al. (2010) Nat. Immunol. 11:1014. McNamee, E.N. et al. (2011) Proc. Natl. Acad. Sci. USA 108:16711. Bufler, P. et al. (2002) Proc. Natl. Acad. Sci. USA 99:13723. Sharma, S. et al. (2008) J. Immunol. 180:5477.
Long Name	Interleukin 37 Isoform B
Entrez Gene IDs	27178 (Human)
Alternate Names	IL-37b, IL37b

Citations for Recombinant Human IL-37b/IL-1F7b Protein (9)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human IL-37b/IL-1F7b Protein include:

Species: Human

Applications: Bioassay

I Kolotilin (2022), Plant-produced recombinant cytokines IL-37b and IL-38 modulate inflammatory response from stimulated human PBMCs Scientific Reports, 12(1):19450.
PMID: 36376518
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

K Ueno-Shuto et al. (2022), A Splice Switch in SIGIRR Causes a Defect of IL-37-Dependent Anti-Inflammatory Activity in Cystic Fibrosis Airway Epithelial Cells International Journal of Molecular Sciences, 23(14).
PMID: 35887095
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

C Jia et al. (2021), IL-37b alleviates endothelial cell apoptosis and inflammation in Kawasaki disease through IL-1R8 pathway Cell Death Disease, 2021;12(6):575.
PMID: 34083516
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

I Tsilioni et al. (2020), IL-38 inhibits microglial inflammatory mediators and is decreased in amygdala of children with autism spectrum disorder Proc. Natl. Acad. Sci. U.S.A., 0(0).
PMID: 32601180
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

I Tsilioni et al. (2019), IL-37 is increased in brains of children with autism spectrum disorder and inhibits human microglia stimulated by neurotensin Proc. Natl. Acad. Sci. U.S.A., 0(0).
PMID: 31591201
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2016), Interleukin-37 expression and its potential role in oral leukoplakia and oral squamous cell carcinoma Sci Rep, 6(0):26757.
PMID: 27225603
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

(2016), Interleukin-37 Enhances the Suppressive Activity of Naturally Occurring CD4(+)CD25(+) Regulatory T Cells Sci Rep, 6(0):38955.
PMID: 27941849
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Wang W et al. (2015), Transfer of the IL-37b gene elicits anti-tumor responses in mice bearing 4T1 breast cancer. Acta Pharmacol Sin, 36(4):528-34.
PMID: 25832432
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Cavalli G et al. (0), Treating experimental arthritis with the innate immune inhibitor interleukin-37 reduces joint and systemic inflammation. Rheumatology (Oxford55(12):2220-2229.
PMID: 27567100

Recombinant Human IL-37b/IL-1F7b Protein

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Product Specifications for Recombinant Human IL-37b/IL-1F7b Protein

Formulation, Preparation and Storage

Carrier Free

7585-IL

Carrier

7585-IL/CF

Carrier Free

Reconstitution Calculator

Background: IL-37b/IL-1F7b

Citations for Recombinant Human IL-37b/IL-1F7b Protein (9)

Product Documents for Recombinant Human IL-37b/IL-1F7b Protein

Product Datasheets

COA

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