Recombinant Human IL-37/IL-1F7 Protein

Name: Recombinant Human IL-37/IL-1F7 Protein
Brand: R&D Systems
SKU: 9225-IL

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 9225-IL

Citations (1)

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Key Product Details

Source:	E. coli
Accession #:	Q9NZH6-2
Applications:	Bioactivity

View Full Technical Details for Recombinant Human IL-37/IL-1F7 Protein

Product Specifications for Recombinant Human IL-37/IL-1F7 Protein

Source	E. coli-derived human IL-37/IL-1F7 protein Ser2-Asp192
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ser2
Predicted Molecular Mass	21 kDa
SDS-PAGE	22 kDa, reducing conditions
Activity	Measured by its ability to inhibit LPS-stimulated IL-8 secretion by HK-2 human kidney epithelial cells. The ED₅₀ for this effect is 0.25-1.25 μg/mL.

Scientific Data Examples for Recombinant Human IL-37/IL-1F7 Protein

	Recombinant Human IL-37/IL-1F7 Protein Bioactivity Recombinant Human IL-37/IL-1F7 (Catalog # 9225-IL) inhibits LPS-stimulated IL-8 secretion by HK-2 human kidney epithelial cells. The ED₅₀ for this effect is 0.25-1.25 μg/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

9225-IL

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS and DTT with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

9225-IL/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS and DTT.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-37/IL-1F7

Human Interleukin-1 family member 7 (IL-1F7), also named FIL-1 zeta, IL-37, IL-1H4, IL-1HL and IL-1RP1, is an anti-inflammatory member of the IL-1 cytokine family (1-3). Alternative splicing generates multiple isoforms (IL-37a through e) with deletions in the N-terminal region of the molecule (4-7). IL-37b shares approximately 21%, 24%, and 30% aa sequence identity with mature IL-1 alpha, IL-1 beta, and IL-1ra, respectively. Mouse IL-37 has not been reported, but human IL-37b is active on mouse cells. Like IL-1 alpha, IL-1 beta and IL-18, all of the IL-37 variants lack a typical signal peptide. IL-37b is up-regulated by inflammatory stimuli in peripheral blood mononuclear cells (8). Experimental over-expression of IL-37b in vivo limits the inflammatory response and protects mice from colitis and LPS-induced shock (8, 9). Both unprocessed and mature IL-37b can form homodimers in solution (8, 10). Although IL-37b will bind to IL-18 R alpha with low affinity, this has no effect on IL‑18 receptor activity (7, 10, 11). Alternatively, IL-37b will also bind to IL-18 BP, generating a complex that interacts with IL-18 R beta. This has the effect of attenuating IL‑18 activity via the IL-18 receptor (11). IL-37b can also function intracellularly. Following LPS-induced cleavage of its propeptide, IL-37b associates with Smad3 and translocates to the nucleus. This results in a reduction of pro-inflammatory cytokine secretion (12).

Item	Value
References	Dinarello, C.A. and P. Bufler (2013) Sem. Immunol. 25:466. Boraschi, D. et al. (2011) Eur. Cytokine Netw. 22:127. Ding, V.A. et al. (2016) Med. Oncol. 33:68. Smith, D.E. et al. (2000) J. Biol Chem 275:1169. Kumar S. et al. (2000) J. Biol Chem 275:10308. Busfield S.J. et al. (2000) Genomics 66:213. Pan, G. et al. (2000) Cytokine 13:1. Nold, M.F. et al. (2010) Nat. Immunol. 11:1014. McNamee, E.N. et al. (2011) Proc. Natl. Acad. Sci. USA 108:16711. Kumar S. et al. (2002) Cytokine 18:61. Bufler, P. et al. (2002) Proc. Natl. Acad. Sci. USA 99:13723. Sharma, S. et al. (2008) J. Immunol. 180:5477.
Long Name	Interleukin 37
Entrez Gene IDs	27178 (Human)
Alternate Names	FIL1, FIL1 zeta, FIL1(ZETA), FIL1ZFIL1 zeta, IL-1F7, IL-1F7IL-1 zeta, IL-1H4IL-1F7b (IL-1H4, IL-1H, IL-1RP1), IL-1RP1IL-1X protein, IL-1X, IL-37, IL1H4IL1F7 (canonical product IL-1F7b), IL1RP1interleukin 1 family member 7, IL37, Interleukin-1 homolog 4, Interleukin-1 zeta, Interleukin-1-related protein, interleukin 1 family, member 7 (zeta), interleukin 1, zeta, interleukin-1 family member 7, interleukin-1 superfamily z

Citations for Recombinant Human IL-37/IL-1F7 Protein (1)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human IL-37/IL-1F7 Protein include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

P Zhao et al. (2021), Single-cell RNA-seq reveals a critical role of novel pro-inflammatory EndMT in mediating adverse remodeling in coronary artery-on-a-chip Science Advances, 7(34).
PMID: 34417174

Recombinant Human IL-37/IL-1F7 Protein

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Human IL-37/IL-1F7 Protein

Scientific Data Examples for Recombinant Human IL-37/IL-1F7 Protein

Formulation, Preparation and Storage

Carrier Free

9225-IL

Carrier

9225-IL/CF

Carrier Free

Reconstitution Calculator

Background: IL-37/IL-1F7

Citations for Recombinant Human IL-37/IL-1F7 Protein (1)

Product Documents for Recombinant Human IL-37/IL-1F7 Protein

Product Datasheets

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