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Recombinant Human IL-36 beta/IL-1F8 Protein

Bio-Techne includes R&D Systems | Catalog # 1099-IL

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Carrier Free
1099-IL-025/CF

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With Carrier
1099-IL-025

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

Binding Activity

Product Specifications

Source

E. coli-derived human IL-36 beta/IL-1F8 protein
Met1-Glu157

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met1

Predicted Molecular Mass

17.7 kDa

Activity

Measured by its binding ability in a functional ELISA.
Immobilized recombinant human IL-36 beta at 1 µg/mL (100 µL/well) can bind recombinant human IL-1 Rrp2 Fc Chimera (Catalog # 872-RP) with a linear range of 0.15-5 µg/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 1099-IL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 1099-IL/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-36 beta/IL-1F8

Human interleukin‑36 beta [IL‑36 beta; previously IL‑1F8 and also named FIL‑1 eta (eta) and IL‑1H2] is a member of the IL‑1 family of proteins (1 ‑ 3, 6). IL‑1 family members include IL‑1 beta, IL‑1 alpha, IL‑1ra, IL‑18, IL‑36Ra/IL‑1F5, IL‑36 alpha/IL‑1F6, IL‑37/IL‑1F7, IL‑36 gamma/IL‑1F9 and IL‑1F10 (4, 6). All family members show a 12 beta‑stranded beta‑trefoil configuration, and are believed to have arisen from a common ancestral gene that has undergone multiple duplications (4). Two alternatively spliced transcript variants encode distinct (164 or 157 residues) protein isoforms that differ in their C‑terminal 70 amino acid (aa) residues have been reported (3). IL‑36 beta/IL‑1F8 isoform 2 is synthesized as a 157 aa protein that contains no signal sequence and no prosegment (1 ‑ 2). Unlike IL‑36 beta/IL‑1F8 isoform 1 which lacks potential N‑linked glycosylation sites, isoform 2 contains one potential N‑linked glycosylation site in its unique C‑terminus. IL‑36 beta/IL‑1F8 is reported to be actively secreted (1). Human IL‑36 beta/IL‑1F8 isoform 2 shares 61% aa identity with mouse IL‑1 ra, a 183 aa form of IL‑36 beta/IL‑1F8. Within the IL‑1 family, IL‑36 beta/IL‑1F8 shares 30%, 32%, 37%, 46%, 34%, 45% and 28% aa sequence identity with IL‑1 ra, IL‑1 beta, IL‑36Ra/IL‑1F5, IL‑36 alpha/IL‑1F6, IL‑37/IL‑1F7, IL‑36 gamma/IL‑1F9 and IL‑1F10, respectively. Cells reported to express IL‑36 beta/IL‑1F8 include resting and activated monocytes and B cells (1, 4). The receptor for IL‑36 beta/IL‑1F8 is reported to be a combination of IL‑1 Rrp2 and IL‑1 RAcP (5). Recombinant IL‑36 beta/IL‑1F8, along with IL‑36 alpha/IL‑1F6 and IL‑36 gamma/IL‑1F9, has been shown to activate the pathway involving NF‑ kappaB and MAPK in an IL‑1 Rrp2 dependent manner.

References

  1. Smith, D.E. et al. (2000) J. Biol. Chem. 275:1169.
  2. Kumar, S. et al. (2000) J. Biol. Chem. 275:10308.
  3. Nicklin, M.J.H. et al. (2002) Genomics 79:718.
  4. Dunn, E. et al. (2001) Trends Immunol. 22:533.
  5. Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.
  6. Dinarello, C. et al. (2010) Nat. Immunol. 11:973.

Long Name

Interleukin 36 beta/Interleukin 1 Family 8

Alternate Names

FIL1 eta, IL-1 eta, IL-1H2, IL1F8, IL36 beta, IL36B

Entrez Gene IDs

27177 (Human); 69677 (Mouse)

Gene Symbol

IL36B

UniProt

Additional IL-36 beta/IL-1F8 Products

Product Documents for Recombinant Human IL-36 beta/IL-1F8 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human IL-36 beta/IL-1F8 Protein

For research use only

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