Recombinant Human IL-36 beta/IL-1F8 Protein

Name: Recombinant Human IL-36 beta/IL-1F8 Protein
Brand: R&D Systems
SKU: 1099-IL

Catalog # 1099-IL

Citations (3)

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New Version Available - 6834-ILB

A New rh IL-36b is Now Available! The new truncated protein is 40-fold more active!

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Key Product Details

Source:	E. coli
Accession #:	Q3MIH0
Applications:	Binding Activity

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Product Specifications

Source	E. coli-derived human IL-36 beta/IL-1F8 protein Met1-Glu157
Purity	>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Met1
Predicted Molecular Mass	17.7 kDa
Activity	Measured by its binding ability in a functional ELISA. Immobilized recombinant human IL-36 beta at 1 µg/mL (100 µL/well) can bind recombinant human IL-1 Rrp2 Fc Chimera (Catalog # 872-RP) with a linear range of 0.15-5 µg/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

1099-IL

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1099-IL/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-36 beta/IL-1F8

Human interleukin‑36 beta [IL‑36 beta ; previously IL‑1F8 and also named FIL‑1 eta (eta) and IL‑1H2] is a member of the IL‑1 family of proteins (1 ‑ 3, 6). IL‑1 family members include IL‑1 beta, IL‑1 alpha, IL‑1ra, IL‑18, IL‑36Ra/IL‑1F5, IL‑36 alpha /IL‑1F6, IL‑37/IL‑1F7, IL‑36 gamma /IL‑1F9 and IL‑1F10 (4, 6). All family members show a 12 beta ‑stranded beta ‑trefoil configuration, and are believed to have arisen from a common ancestral gene that has undergone multiple duplications (4). Two alternatively spliced transcript variants encode distinct (164 or 157 residues) protein isoforms that differ in their C‑terminal 70 amino acid (aa) residues have been reported (3). IL‑36 beta /IL‑1F8 isoform 2 is synthesized as a 157 aa protein that contains no signal sequence and no prosegment (1 ‑ 2). Unlike IL‑36 beta /IL‑1F8 isoform 1 which lacks potential N‑linked glycosylation sites, isoform 2 contains one potential N‑linked glycosylation site in its unique C‑terminus. IL‑36 beta /IL‑1F8 is reported to be actively secreted (1). Human IL‑36 beta /IL‑1F8 isoform 2 shares 61% aa identity with mouse IL‑1 ra, a 183 aa form of IL‑36 beta /IL‑1F8. Within the IL‑1 family, IL‑36 beta /IL‑1F8 shares 30%, 32%, 37%, 46%, 34%, 45% and 28% aa sequence identity with IL‑1 ra, IL‑1 beta, IL‑36Ra/IL‑1F5, IL‑36 alpha /IL‑1F6, IL‑37/IL‑1F7, IL‑36 gamma /IL‑1F9 and IL‑1F10, respectively. Cells reported to express IL‑36 beta /IL‑1F8 include resting and activated monocytes and B cells (1, 4). The receptor for IL‑36 beta /IL‑1F8 is reported to be a combination of IL‑1 Rrp2 and IL‑1 RAcP (5). Recombinant IL‑36 beta /IL‑1F8, along with IL‑36 alpha /IL‑1F6 and IL‑36 gamma /IL‑1F9, has been shown to activate the pathway involving NF‑ kappa B and MAPK in an IL‑1 Rrp2 dependent manner.

Item	Value
References	Smith, D.E. et al. (2000) J. Biol. Chem. 275:1169. Kumar, S. et al. (2000) J. Biol. Chem. 275:10308. Nicklin, M.J.H. et al. (2002) Genomics 79:718. Dunn, E. et al. (2001) Trends Immunol. 22:533. Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677. Dinarello, C. et al. (2010) Nat. Immunol. 11:973.
Long Name	Interleukin 36 beta/Interleukin 1 Family 8
Entrez Gene IDs	27177 (Human); 69677 (Mouse)
Alternate Names	FIL1, FIL1 eta, FIL1-(ETA), FIL1H, FILI-(ETA), IL-1 eta, IL-1F8, IL-1F8 (FIL1-eta), IL-1H2, IL-36 beta, IL1-ETA, IL1F8, IL1F8 (Canonical product IL-1F8a), IL1H2, IL36 beta, IL36B, Interleukin 36, Beta, Interleukin-1 Superfamily e, Interleukin-1 eta, Interleukin-1 homolog 2, Interleukin-36 Beta, family of interleukin 1-eta, interleukin 1 family, member 8 (eta), interleukin 1, eta, interleukin-1 family member 8

Citations for Recombinant Human IL-36 beta/IL-1F8 Protein (3)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human IL-36 beta/IL-1F8 Protein include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Wilson TR et al. (2012), Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 487(7408):505-9.
PMID: 22763448
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Carrier Y et al. (2011), Inter-regulation of Th17 cytokines and the IL-36 cytokines in vitro and in vivo: implications in psoriasis pathogenesis. J. Invest. Dermatol., 131(12):2428-37.
PMID: 21881584
Magne D et al. (2006), The new IL-1 family member IL-1F8 stimulates production of inflammatory mediators by synovial fibroblasts and articular chondrocytes. Arthritis Res. Ther., 8(3):R80.
PMID: 16646978

Recombinant Human IL-36 beta/IL-1F8 Protein

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Product Specifications

Formulation, Preparation and Storage

Carrier Free

1099-IL

Carrier

1099-IL/CF

Carrier Free

Reconstitution Calculator

Background: IL-36 beta/IL-1F8

Citations for Recombinant Human IL-36 beta/IL-1F8 Protein (3)

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