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Recombinant Human IL-31RA Fc Chimera Protein, CF

Catalog # 11028-IL | R&D Systems, Inc. a Bio-Techne Brand
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11028-IL-050

Key Product Details

Source

HEK293

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human IL-31RA protein
Human IL-31RA
(Ala20-Ser516)
Accession # Q8NI17.1
DIEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala20

Predicted Molecular Mass

83 kDa

SDS-PAGE

114-131 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human IL-31 (Catalog # 2824-IL/CF) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human IL-31RA Fc Chimera (Catalog # 11028-IL) binds with an ED50 of 8.00-64.0 ng/mL.

Scientific Data Images for Recombinant Human IL-31RA Fc Chimera Protein, CF

Recombinant Human IL-31RA Fc Chimera Protein Binding Activity.

When Recombinant Human IL-31 Protein (2824-IL/CF) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human IL-31RA Fc Chimera Protein (Catalog # 11028-IL) binds with an ED50 of 8.00-64.0 ng/mL.

Recombinant Human IL-31RA Fc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Human IL-31RA Fc Chimera Protein (Catalog # 11028-IL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 114-131 kDa and 130-160 kDa, respectively.

Formulation, Preparation and Storage

11028-IL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-31RA

The interleukin-31 receptor A subunit (IL-31 RA), also known as gp130-Like Monocyte Receptor (GLM-R or GPL), is a ~100 kDa type I transmembrane glycoprotein that is classified as being a type I cytokine receptor (1, 2). A heterodimeric complex of IL-31 RA and the oncostatin M receptor (OSM-R) functions as the signaling receptor for IL-31 (3). Both subunits are inducibly expressed throughout the myelomonocytic lineage and are upregulated by interferon-gamma and bacterial lipopolysaccharides (1-3). IL-31 RA is also expressed on keratinocytes, dorsal root ganglia neurons, and variably on lung epithelial cells (3-6). The 732 amino acid (aa) IL-31 RA contains a 19 aa signal sequence, a 500 aa extracellular domain (ECD), a 21 aa transmembrane domain and a 192 aa cytoplasmic domain. The ECD shares 60%, 58%, 73% and 70% aa identity with mouse, rat, canine and bovine IL-31 RA ECD, respectively. Human IL-31 receptors do not respond to mouse IL-31 (7). The ECD contains five fibronectin type III domains; the first two contain four conserved cysteine residues and a WSXWS motif common to type I cytokine receptors (2). Twelve alternately spliced human IL-31 RA isoforms are known and range in size from 356-745 amino acids. A long (745 aa) and a short (560 aa) transmembrane form are the predominant forms, and many cell lines express both forms (8). The long form, like the 732 aa form, signals by recruiting STAT3, 5 or 1, while the short form does not recruit STATs and inhibits IL-31 signaling. The ratio of these forms and their co-expression with OSM-R determines a cell's response to IL-31 (8). In both humans and transgenic mice, IL-31 from skin-homing Th2 cells may contribute to the pruritis (itching) associated with nonatopic dermatitis, especially in infected skin (3, 9, 10). 

References

  1. Ghilardi, N. et al. (2002) J. Biol. Chem. 277:16831.
  2. Diveu, C. et al. (2003) J. Biol. Chem. 278:49850.
  3. Dillon, S. R. et al. (2004) Nat. Immunol. 5:752.
  4. Chattopadhyay, S. et al. (2007) J. Biol. Chem. 282:3014.
  5. Perrigoue, J. G. et al. (2007) J. Exp. Med. 204:481.
  6. Bando, T. et al. (2006) Neuroscience 142:1263.
  7. Broxmeyer, H. E. et al. (2007) Exp. Hematol. 35:78.
  8. Diveu, C. et al. (2004) Eur. Cytokine. Netw. 15:291.
  9. Bilsborough, J. et al. (2006) J. Allergy Clin. Immunol. 117:418.
  10. Sonkoly, E. et al. (2006) J. Allergy Clin. Immunol. 117:411.

Long Name

Interleukin 31 Receptor A

Alternate Names

CRL3, GLM-R, IL-31 RA, IL31RA

Entrez Gene IDs

133396 (Human); 218624 (Mouse)

Gene Symbol

IL31RA

UniProt

Additional IL-31RA Products

Product Documents for Recombinant Human IL-31RA Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human IL-31RA Fc Chimera Protein, CF

For research use only

Reconstitution Calculator

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