Recombinant Human IL-21R Fc Chimera Protein, CF

Name: Recombinant Human IL-21R Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 991-R2

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 991-R2

Citations (15)

Product Datasheet / COA / SDS

New Version Available - 9249-R2

A New rh IL-21 R is Available! It is HEK293 expressed, exhibits enhanced activity, and has a His-tag.

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Setting the standard in quality research reagents

Key Product Details

Source:	NS0
Accession #:	Q9HBE5
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

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Product Specifications for Recombinant Human IL-21R Fc Chimera Protein, CF

Source

Mouse myeloma cell line, NS0-derived human IL-21R protein

Human IL-21 R Subunit (Cys20-Pro236) Accession # Q9HBE5	DIEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Cys20

Predicted Molecular Mass

51.7 kDa (monomer)

SDS-PAGE

80-85 kDa, reducing conditions

Activity

Measured by its ability to inhibit IL-21-dependent enhancement of IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The ED₅₀ for this effect is 40-400 ng/mL.

Scientific Data Examples for Recombinant Human IL-21R Fc Chimera Protein, CF

	Measured by its ability to inhibit IL-21-dependent enhancement of IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The ED₅₀ for this effect is 40-400 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

991-R2

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-21R

Interleukin-21 Receptor (IL-21 R) is a type I transmembrane glycoprotein within the class I cytokine receptor family (1). IL-21 R associates with the common gamma chain ( gamma _c) which is also a component of the receptors for IL-2, IL-4, IL-7, IL-9, IL-13, and IL-15 (2, 3). Mature human IL-21 R consists of a 213 amino acid (aa) extracellular domain (ECD) with 4 conserved cysteine residues, a fibronectin type III domain, and a WSxWS motif, followed by a 21 aa transmembrane domain and a 253 aa cytoplasmic domain with a Box 1 motif, a kinase domain, and several sites for tyrosine phosphorylation (4, 5). Within the ECD, human IL-21 R shares 69% aa identity with mouse and rat IL-21 R, respectively. IL-21 R is expressed mainly on B cells (highest on mature, activated, follicular and germinal center B cells), NK cells, and activated T cells, but is also found on dendritic cells, alternatively activated macrophages, intestinal lamina propria fibroblasts and epithelial cells, and keratinocytes (1, 4, 5). Both IL-21 and IL-4 are necessary for efficient B cell IgG1 production and normal germinal center architecture (6). B cell IL-21 R engagement induces Blimp-1 (which mediates plasma cell differentiation) and is important for memory responses (7, 8). IL-21 R engagement enhances NK cell mediated cytotoxic activity and IFN-gamma production (4, 9), control of viral infection and tumor growth by CD8⁺ T cells (10), development of regulatory T cells (11), IL-23 responsiveness of encephalitogenic Th17 cells (12), but suppresses the accumulation of IL-17 secreting gamma δ T cells in the airway (13). IL-21 R expression is often upregulated in allergic skin inflammation, systemic lupus erythematosus and diffuse large B cell lymphoma (DLBCL) (14, 15).

Item	Value
References	Tangye, S.G. (2015) Curr. Opin. Immunol. 34:107. Asao, H. et al. (2001) J. Immunol. 167:1. Habib, T. et al. (2002) Biochemistry 41:8725. Parrish-Novak, et al. (2000) Nature 408:57. Ozaki, K. et al. (2000) Proc. Natl. Acad. Sci. USA 97:11439. Ozaki, K. et al. (2002) Science 298:1630. Rankin, A.L. et al. (2011) J. Immunol. 186:667. King, I.L. et al. (2010) J. Immunol. 185:6138. Kasaian, M.T. et al. (2002) Immunity 16:559. Frohlich, A. et al. (2009 Science 324:1576. Tortola, L. et al. (2010) Blood 116:5200. Lee, Y. et al. (2015) J. Clin. Invest. 125:4011. Moser, E.K. et al. (2015) PLoS One 10:e0120169. Jin, H. et al. (2009) J. Clin. Invest. 119:47. Sarosiek, K.A. et al. (2010) Blood 115:570.
Long Name	Interleukin 21 Receptor
Entrez Gene IDs	50615 (Human); 60504 (Mouse); 102135042 (Cynomolgus Monkey); 705759 (Rhesus Macaque)
Alternate Names	CD360, CD360 antigen, IL-21 R, IL-21 receptor, IL-21R, IL21R, MGC10967, NILR, Novel interleukin receptor, interleukin 21 receptor, interleukin-21 receptor

Citations for Recombinant Human IL-21R Fc Chimera Protein, CF (15)

Showing 1 - 10 of 15 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human IL-21R Fc Chimera Protein, CF include:

Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

K Szabó et al. (2022), Altered Circulating Follicular T Helper Cell Subsets and Follicular T Regulatory Cells Are Indicators of a Derailed B Cell Response in Lupus, Which Could Be Modified by Targeting IL-21R International Journal of Molecular Sciences, 23(20).
PMID: 36293075
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

E Janssen et al. (2020), DOCK8 is essential for LFA-1 dependent positioning of T follicular helper cells in germinal centers JCI Insight, 0(0).
PMID: 32573493
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

O Désy et al. (2018), Allogeneic dendritic cells stimulated with antibodies against HLA class II polarize naive T cells in a follicular helper phenotype Sci Rep, 8(1):4025.
PMID: 29507364
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

A Aljurayyan et al. (2018), Activation and induction of antigen-specific T follicular helper cells (TFH) play a critical role in LAIV-induced human mucosal anti-influenza antibody response J. Virol., 0(0).
PMID: 29563292
Species: N/A
Sample Types: Recombinant Protein
Applications: Bioassay

YM Kooy-Winke et al. (2017), CD4 T-cell cytokines synergize to induce proliferation of malignant and nonmalignant innate intraepithelial lymphocytes Proc. Natl. Acad. Sci. U.S.A, 0(0).
PMID: 28049849
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2016), Augmentation of Circulating Follicular Helper T Cells and Their Impact on Autoreactive B Cells in Myasthenia Gravis J Immunol, 0(0).
PMID: 27543617
Species: Mouse
Sample Types: Whole Cells
Applications: IHC

Harker J et al. (2016), Cell-Intrinsic gp130 Signaling on CD4+ T Cells Shapes Long-Lasting Antiviral Immunity. J Immunol, 195(3):1071-81.
PMID: 26085685
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Kaltenmeier C et al. (2015), CD4+ T cell-derived IL-21 and deprivation of CD40 signaling favor the in vivo development of granzyme B-expressing regulatory B cells in HIV patients. J Immunol, 194(8):3768-77.
PMID: 25780036
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Xing R et al. (2015), Interleukin-21 Induces Proliferation and Proinflammatory Cytokine Profile of Fibroblast-like Synoviocytes of Patients with Rheumatoid Arthritis. Scand J Immunol, 83(1):64-71.
PMID: 26482544
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Parmigiani A et al. (2013), Impaired antibody response to influenza vaccine in HIV-infected and uninfected aging women is associated with immune activation and inflammation. PLoS ONE, 8(11):e79816.
PMID: 24236161
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Mazerolles F et al. (2013), Blood CD4+CD45RO+CXCR5+ T cells are decreased but partially functional in signal transducer and activator of transcription 3 deficiency. J Allergy Clin Immunol, 131(4):1146-56, 1156.
PMID: 23403044
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Kasagi S et al. (2010), Anti-programmed cell death 1 antibody reduces CD4+PD-1+ T cells and relieves the lupus-like nephritis of NZB/W F1 mice. J. Immunol., 184(5):2337-47.
PMID: 20139271
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Cerboni C et al. (2009), Detuning CD8+ T lymphocytes by down-regulation of the activating receptor NKG2D: role of NKG2D ligands released by activated T cells. Blood, 113(13):2955-64.
PMID: 19124832
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Eid RE et al. (2009), Interleukin-17 and interferon-gamma are produced concomitantly by human coronary artery-infiltrating T cells and act synergistically on vascular smooth muscle cells. Circulation, 119(10):1424-32.
PMID: 19255340
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Monteleone G et al. (2006), Control of matrix metalloproteinase production in human intestinal fibroblasts by interleukin 21. Gut, 55(12):1774-80.
PMID: 16682426

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