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Recombinant Human IL-17F (NS0-expressed) Protein

Catalog # 1335-INS | R&D Systems, Inc. a Bio-Techne Brand
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Carrier Free
1335-INS-010/CF
1335-INS-025/CF

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Availability
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With Carrier
1335-INS-025

Key Product Details

Source

NS0

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human IL-17F protein
Arg31-Gln163, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg31

Predicted Molecular Mass

15.7 kDa (monomer)

SDS-PAGE

21 kDa, reducing conditions

Activity

Measured by its ability to induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells.
The ED50 for this effect is 2-10 ng/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 1335-INS
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 1335-INS/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17F

Interleukin-17F (also known as ML-1) is a of the IL-17 family of cytokines. Members of this family are involved in tissue homeostasis and demonstrate a structural motif termed a cysteine-knot that characterizes a large superfamily of growth factors. Although most cysteine-knot superfamily members use three intrachain disulfide bonds to create a knot, IL-17 family molecules generate the same structural form with only two disulfide links (1 - 3). Human IL-17F is secreted as a glycosylated, 45 kDa, disulfide-linked homodimer. It is synthesized as a 153 amino acid (aa) precursor that contains a 20 aa signal sequence and a 133 aa mature region. As noted, the molecule contains a cysteine-knot that is formed from two sets of paired beta-strands that are connected by disulfide linkages (4 - 6). There is potentially one additional isoform that utilizes an alternate start site. This adds an additional 10 aa at the N-terminus (1, 7). 

When induced, IL-17F is preferentially translated as the 153 aa precursor (1). Initially, IL-17F was also reported as IL-24 (7). Since that time, the IL-24 designation has been reassigned to MDA-7, a member of the IL-10 family of molecules (note: IL-17E is synonymous with IL-25). Mature human IL-17F is 59%, 56%, 44% and 52% aa identical to mature rat, mouse, canine and bovine IL-17F, respectively. Within the IL-17 family, IL-17F is most closely related to IL-17/IL-17A at 50% aa identity (6). Human IL-17F is active on mouse and porcine cells (6). IL-17F is produced by activated CD4+ T cells, mast cells, basophils and monocytes (1, 4), and is inducible by IL-23 (4, 8). Targets for IL-17F include respiratory epithelium and endothelial cells which produce proinflammatory cytokines that promote neutrophil activation and influx (4, 5, 9, 10).

References

  1. Kawaguchi, M. et al. (2004) J. Allergy Clin. Immunol. 114:1265.
  2. Kolls, J.K. and A. Linden (2004) Immunity 21:467.
  3. Moseley, T.A. et al. (2003) Cytokine Growth Factor Rev. 14:155.
  4. Kawaguchi, M. et al. (2001) J. Immunol. 167:4430.
  5. Starnes, T. et al. (2001) J. Immunol. 167:4137.
  6. Hymowitz, S.G. et al. (2001) EMBO J. 20:5332.
  7. Hromes, R.A. and T.T. Starnes (2001) GenBank
    Accession # AAK83350.
  8. Langrish, C.L. et al. (2005) J. Exp. Med. 201:233.
  9. Mcallister, F. et al. (2005) J. Immunol. 175:404.
  10. Numasaki, M. et al. (2004) Immunol. Lett. 95:175.

Long Name

Interleukin 17F

Alternate Names

IL17F

Entrez Gene IDs

112744 (Human); 257630 (Mouse); 301291 (Rat); 708220 (Rhesus Macaque)

Gene Symbol

IL17F

UniProt

Additional IL-17F Products

Product Documents for Recombinant Human IL-17F (NS0-expressed) Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human IL-17F (NS0-expressed) Protein

For research use only

Reconstitution Calculator

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