Recombinant Human IL-16 Protein

Name: Recombinant Human IL-16 Protein
Brand: R&D Systems
SKU: 316-IL
Rating: 5 (2 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 316-IL

Citations (5)

(2)

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Key Product Details

Source:	E. coli
Accession #:	AAC12732
Applications:	Bioactivity

View Full Technical Details for Recombinant Human IL-16 Protein

Product Specifications for Recombinant Human IL-16 Protein

Source	E. coli-derived human IL-16 protein Pro2-Ser130
Purity	>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Pro2
Predicted Molecular Mass	13.3 kDa
SDS-PAGE	18.5 kDa, reducing conditions
Activity	Bioassay data are not available.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

316-IL

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

316-IL/CF

Carrier Free

Formulation	Supplied as a 0.2 μm filtered solution in PBS.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after opening.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-16

Interleukin 16, also named lymphocyte chemoattractant factor (LCF), was originally identified as a CD8⁺ T-cell-derived chemoattractant for CD4⁺ cells. The biologically active form of IL-16 was originally proposed to be a homotetramer of 14 kDa chains containing 130 amino acid residue subunits. The complete pro-IL-16 cDNA was subsequently cloned and shown to encode a 631 amino acid residue hydrophilic protein that lacked a signal peptide. The original 130 amino acid residue polypeptide is now believed to have been derived from the C terminus of the precursor. IL-16 precursor protein has been detected in the lysates of various cells including mitogen stimulated PBMCs. The biologically active and secreted natural IL-16 is assumed to be a proteolytic cleavage product of pro-IL-16 generated by proteases present in or on activated CD8⁺ cells. A likely cleavage site was proposed to be at aspartate residue 510. This would yield a 121 amino acid residue protein, smaller than the 130 aa residue protein first described. The expression of IL-16 precursor mRNA has been detected in various tissues including spleen, thymus, lymph nodes, peripheral leukocytes, bone marrow and cerebellum. The gene for IL-16 precursor has been localized to chromosome 15. The biological activities ascribed to IL-16 are reported to be dependent on the cell surface expression of CD4, suggesting that IL-16 is a CD4 ligand. Besides its chemotactic properties, IL-16 has also been shown to suppress HIV-1 replication in vitro. Recombinant E. coli-derived IL-16 produced at R&D Systems is present mostly as a monomer, exhibits chemotactic activity for lymphocytes at high concentrations, lacks chemotactic activites for monocytes, and binds the extracellular domain of CD4 with low affinity.

Item	Value
References	Cruikshank, W.W. et al. (1994) Proc. Natl. Acad. Sci. USA 91:5109. Baier, M. et al. (1997) Proc. Natl. Acad. Sci. USA 94:5273. Zhou, A. et al. (1997) Nature Medicine 3:659. Bazan, J.F. and T.J. Schall (1996) Nature 381:29.
Long Name	Interleukin 16
Entrez Gene IDs	3603 (Human); 16170 (Mouse)
Alternate Names	FLJ16806, FLJ42735, FLJ44234, HsT19289, IL-16, IL16, LCF, LCFprIL-16, NIL16, PRIL16, interleukin 16 (lymphocyte chemoattractant factor), lymphocyte chemoattractant factor, neuronal interleukin 16, pro-interleukin-16, prointerleukin 16

Citations for Recombinant Human IL-16 Protein (5)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human IL-16 Protein include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Ghigo E et al. (2010), IL-16 promotes T. whipplei replication by inhibiting phagosome conversion and modulating macrophage activation. PLoS ONE, 5(10):e13561.
PMID: 21042409
Species: N/A
Sample Types: N/A
Applications: ELISA (Standard)

Biancotto A et al. (2008), Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo. AIDS Res. Hum. Retroviruses, 24(3):453-62.
PMID: 18327985
Species: N/A
Sample Types: N/A
Applications: ELISA (Standard)

Biancotto A et al. (2007), Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1. Blood, 109(10):4272-9.
PMID: 17289812
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Kao CY et al. (2004), IL-17 markedly up-regulates beta-defensin-2 expression in human airway epithelium via JAK and NF-kappaB signaling pathways. J. Immunol., 173(5):3482-91.
PMID: 15322213
Species: N/A
Sample Types: N/A
Applications: ELISA (Standard)

Bottcher MF et al. (2000), Chemoattractant factors in breast milk from allergic and nonallergic mothers. Pediatr. Res., 47(5):592-7.
PMID: 10813582

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Customer Reviews for Recombinant Human IL-16 Protein (2)

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Recombinant Human IL-16 Protein

Name: Anonymous

Application: Immunoassay Standard

Verified Customer | Posted 08/20/2021
Recombinant Human IL-16 Protein

Name: Anonymous

Application: Immunoassay Standard

Verified Customer | Posted 11/16/2017

A sandwich ELISA was made with MAB316 as the capture and biotinylated AF-316 as the detection using 316-IL as the immunoassay calibrator.

Recombinant Human IL-16 Protein

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Product Specifications for Recombinant Human IL-16 Protein

Formulation, Preparation and Storage

Carrier Free

316-IL

Carrier

316-IL/CF

Carrier Free

Reconstitution Calculator

Background: IL-16

Citations for Recombinant Human IL-16 Protein (5)

Customer Reviews for Recombinant Human IL-16 Protein (2)

Customer Images

Recombinant Human IL-16 Protein

Recombinant Human IL-16 Protein

Product Documents for Recombinant Human IL-16 Protein

Product Datasheets

COA

Certificate of Analysis

SDS

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