Recombinant Human IGSF8/CD316 Fc Chimera Protein, CF

Catalog # 1241-S8 | R&D Systems, Inc. a Bio-Techne Brand
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Key Product Details

Accession #

Q969P0-1

Source

HEK293

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human IGSF8/CD316 protein
Human IGSF8
(Arg28-Thr579)
Accession # Q969P0-1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg28

Predicted Molecular Mass

85 kDa

SDS-PAGE

86-113 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human IGSF8/CD316 Fc Chimera is immobilized at 1 µg/mL (100 µL/well), Biotinylated Recombinant CD81 Fc Chimera binds with an ED50 of 1.2-7.2 μg/mL

Scientific Data Images for Recombinant Human IGSF8/CD316 Fc Chimera Protein, CF

Recombinant Human IGSF8/CD316 Fc Chimera Protein Binding Activity

Recombinant Human IGSF8/CD316 Fc Chimera Protein Binding Activity

When Recombinant Human IGSF8/CD316 Fc Chimera (Catalog # 1241-S8) is immobilized at 1 µg/mL (100 µL/well), Biotinylated Recombinant CD81 Fc Chimera binds with an ED50 of 1.2-7.2 μg/mL.
Recombinant Human IGSF8/CD316 Fc Chimera Protein SDS-PAGE

Recombinant Human IGSF8/CD316 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Human IGSF8/CD316 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 86-113 kDa and 170-230 kDa, respectively.

Formulation, Preparation and Storage

1241-S8
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: IGSF8/CD316

IGSF8 (Immunoglobulin superfamily member 8), also known as EWI-2, KCT-4, LIR-D1, and PGRL, is a 75-kDa cell surface protein belonging to the immunoglobulin superfamily (1). IGSF8 is widely expressed, with pronounced mRNA expression in the brain and protein expression on peripheral blood lymphocytes and hepatocytes where it colocalizes with CD81 (1-3). It strongly associates with tetraspanins CD9 and CD81 which may act as physical linkers to form a complex with alpha3 beta1 integrin that may regulate cell aggregation and motility on laminin-5 (4). Human IGSF8 is synthesized as a 613 aa protein that includes a 27 aa signal peptide, a 552 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 13 aa cytoplasmic tail. Within the ECD, human IGSF8 shares 91% and 90% aa sequence identity with mouse and rat IGSF8, respectively. IGSF8 is an inducible receptor for Heat Shock Protein A8 (HSPA8) on activated dendritic cells (5). IGSF8 can interact with alpha-Actinin to regulate T cell immune synapses and HIV viral infection (6). In human glioma patients, low IGSF8 expression correlates with shorter survival time. Studies have shown that re-expression of IGSF8 in malignant glioblastoma cell lines inhibited glioblastoma colony formation in soft agar and caused diminished cell motility and invasion (7).

References

  1. Clark, K.L. et al. (2001) J. Immunol. 167:5115.
  2. Stipp, C.S. et al. (2001) J. Biol. Chem. 276:40545.
  3. Charrin, S. et al. (2003) Biochem. J. 373:409.
  4. Stipp, C.S. et al. (2003) J. Cell Biol. 163:1167.
  5. Kettner, S. et al. (2007) Mol Cell Biol. 27:7718.
  6. Gordón-Alonso, M. et al. (2012) J Immunol 189:689.
  7. Kolesnikova, T. et al. (2009) Neoplasia.11:77.

Long Name

Immunoglobulin Superfamily, Member 8

Alternate Names

CD316, CD81P3, EWI2, KCT4, PGRL

Entrez Gene IDs

93185 (Human); 140559 (Mouse)

Gene Symbol

IGSF8

UniProt

Q969P0-1

Product Documents for Recombinant Human IGSF8/CD316 Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Human IGSF8/CD316 Fc Chimera Protein, CF

For research use only

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